BRENDA - Enzyme Database show
show all sequences of 1.1.1.306

The metabolism of nitrosothiols in the Mycobacteria: identification and characterization of S-nitrosomycothiol reductase

Vogt, R.N.; Steenkamp, D.J.; Zheng, R.; Blanchard, J.S.; Biochem. J. 374, 657-666 (2003)

Data extracted from this reference:

KM Value [mM]
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.0173
-
mycothiol
pH 8.7, 30°C
Mycolicibacterium smegmatis
0.354
-
NAD+
pH 8.7, 30°C
Mycolicibacterium smegmatis
Molecular Weight [Da]
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
38263
-
x * 38263, electrospray mass spectrometry
Mycolicibacterium smegmatis
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Mycolicibacterium smegmatis
-
-
-
Mycolicibacterium smegmatis mc2
-
-
-
Purification (Commentary)
Commentary
Organism
-
Mycolicibacterium smegmatis
Source Tissue
Source Tissue
Commentary
Organism
Textmining
cell culture
-
Mycolicibacterium smegmatis
-
Specific Activity [micromol/min/mg]
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
22.64
-
-
Mycolicibacterium smegmatis
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
S-hydroxymethylmycothiol + NAD+
rapid-equilibrium ordered mechanism
654527
Mycolicibacterium smegmatis
formic acid + mycothiol + NADH + ?
-
-
-
?
S-hydroxymethylmycothiol + NAD+
rapid-equilibrium ordered mechanism
654527
Mycolicibacterium smegmatis mc2
formic acid + mycothiol + NADH + ?
-
-
-
?
S-nitrosomycothiol + NADH
decomposition with a sequential mechanism, enzyme reduces the nitroso group to the oxidation level of nitroxyl
654527
Mycolicibacterium smegmatis
?
-
-
-
?
S-nitrosomycothiol + NADH
decomposition with a sequential mechanism, enzyme reduces the nitroso group to the oxidation level of nitroxyl
654527
Mycolicibacterium smegmatis mc2
?
-
-
-
?
Subunits
Subunits
Commentary
Organism
?
x * 38263, electrospray mass spectrometry
Mycolicibacterium smegmatis
Turnover Number [1/s]
Turnover Number Minimum [1/s]
Turnover Number Maximum [1/s]
Substrate
Commentary
Organism
Structure
additional information
-
additional information
-
Mycolicibacterium smegmatis
pH Optimum
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
8
9.5
optimal in Na-diphosphate buffer, buffers containing amino groups give poor activity, possibly because of Schiff base formation with formaldehyde
Mycolicibacterium smegmatis
Cofactor
Cofactor
Commentary
Organism
Structure
NAD+
-
Mycolicibacterium smegmatis
Cofactor (protein specific)
Cofactor
Commentary
Organism
Structure
NAD+
-
Mycolicibacterium smegmatis
KM Value [mM] (protein specific)
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.0173
-
mycothiol
pH 8.7, 30°C
Mycolicibacterium smegmatis
0.354
-
NAD+
pH 8.7, 30°C
Mycolicibacterium smegmatis
Molecular Weight [Da] (protein specific)
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
38263
-
x * 38263, electrospray mass spectrometry
Mycolicibacterium smegmatis
Purification (Commentary) (protein specific)
Commentary
Organism
-
Mycolicibacterium smegmatis
Source Tissue (protein specific)
Source Tissue
Commentary
Organism
Textmining
cell culture
-
Mycolicibacterium smegmatis
-
Specific Activity [micromol/min/mg] (protein specific)
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
22.64
-
-
Mycolicibacterium smegmatis
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
S-hydroxymethylmycothiol + NAD+
rapid-equilibrium ordered mechanism
654527
Mycolicibacterium smegmatis
formic acid + mycothiol + NADH + ?
-
-
-
?
S-hydroxymethylmycothiol + NAD+
rapid-equilibrium ordered mechanism
654527
Mycolicibacterium smegmatis mc2
formic acid + mycothiol + NADH + ?
-
-
-
?
S-nitrosomycothiol + NADH
decomposition with a sequential mechanism, enzyme reduces the nitroso group to the oxidation level of nitroxyl
654527
Mycolicibacterium smegmatis
?
-
-
-
?
S-nitrosomycothiol + NADH
decomposition with a sequential mechanism, enzyme reduces the nitroso group to the oxidation level of nitroxyl
654527
Mycolicibacterium smegmatis mc2
?
-
-
-
?
Subunits (protein specific)
Subunits
Commentary
Organism
?
x * 38263, electrospray mass spectrometry
Mycolicibacterium smegmatis
Turnover Number [1/s] (protein specific)
Turnover Number Minimum [1/s]
Turnover Number Maximum [1/s]
Substrate
Commentary
Organism
Structure
additional information
-
additional information
-
Mycolicibacterium smegmatis
pH Optimum (protein specific)
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
8
9.5
optimal in Na-diphosphate buffer, buffers containing amino groups give poor activity, possibly because of Schiff base formation with formaldehyde
Mycolicibacterium smegmatis
Other publictions for EC 1.1.1.306
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
724589
Yoshida
Gene expression analysis of me ...
Rhodococcus erythropolis, Rhodococcus erythropolis N9T-4
Biosci. Biotechnol. Biochem.
75
123-127
2011
-
-
1
-
-
-
-
-
-
-
-
2
-
3
-
-
-
-
-
-
-
-
2
-
1
-
-
-
1
-
-
1
-
-
-
-
-
1
1
-
-
-
-
-
-
-
-
-
-
2
-
-
-
-
-
-
-
-
2
-
1
-
-
-
1
-
-
-
2
1
1
2
-
-
690518
Lee
Thiols in nitric oxide synthas ...
Nocardia sp., Nocardia sp. NRRL 5646
Appl. Environ. Microbiol.
73
3095-3097
2007
1
1
-
-
-
-
-
-
-
-
-
-
-
6
-
-
-
-
-
-
-
-
2
-
-
-
-
-
-
-
-
1
-
-
-
1
1
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
654527
Vogt
The metabolism of nitrosothiol ...
Mycolicibacterium smegmatis, Mycolicibacterium smegmatis mc2
Biochem. J.
374
657-666
2003
-
-
-
-
-
-
-
2
-
-
1
-
-
5
-
-
1
-
-
1
1
-
4
1
-
-
-
1
1
-
-
1
-
-
-
-
-
-
1
-
-
-
-
-
-
2
-
-
1
-
-
-
-
1
-
1
1
-
4
1
-
-
-
1
1
-
-
-
-
-
-
-
-
-
288322
Misset-Smits
Mycothiol, 1-O-(2'-[N-acwtyl-L ...
Amycolatopsis methanolica, Rhodococcus erythropolis
FEBS Lett.
409
221-222
1997
-
-
-
-
-
-
1
-
-
-
-
2
-
5
-
-
-
-
-
2
1
-
2
-
-
-
-
-
-
-
-
4
-
-
-
-
-
-
4
-
-
-
-
1
-
-
-
-
-
2
-
-
-
-
-
2
1
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
288323
Norin
Mycothiol-dependent formaldehy ...
Amycolatopsis methanolica
Eur. J. Biochem.
248
282-289
1997
-
-
-
-
-
-
-
-
-
-
-
1
-
2
-
-
1
-
-
1
-
-
1
-
-
-
-
-
-
-
-
2
-
-
-
-
-
-
2
-
-
-
-
-
-
-
-
-
-
1
-
-
-
1
-
1
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
288324
Van Ophem
NAD-linked, factor-dependent f ...
Amycolatopsis methanolica
Eur. J. Biochem.
206
511-518
1992
2
-
-
-
-
-
9
8
-
1
2
1
-
7
-
-
1
-
-
1
4
-
3
1
-
-
-
-
2
-
-
1
-
-
-
2
-
-
1
-
-
-
-
9
-
8
-
1
2
1
-
-
-
1
-
1
4
-
3
1
-
-
-
-
2
-
-
-
-
-
-
-
-
-