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Gene expression analysis of methylotrophic oxidoreductases involved in the oligotrophic growth of Rhodococcus erythropolis N9T-4
Yoshida, N.; Hayasaki, T.; Takagi, H.; Biosci. Biotechnol. Biochem. 75, 123-127 (2011)
Data extracted from this reference:
Cloned(Commentary)
Commentary
Organism
mFADH genetic organization, overview
Rhodococcus erythropolis
Natural Substrates/ Products (Substrates)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
S-(hydroxymethyl)mycothiol + NAD+
Rhodococcus erythropolis
-
S-formylmycothiol + NADH + H+
-
-
?
S-(hydroxymethyl)mycothiol + NAD+
Rhodococcus erythropolis N9T-4
-
S-formylmycothiol + NADH + H+
-
-
?
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Rhodococcus erythropolis
-
-
-
Rhodococcus erythropolis N9T-4
-
-
-
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
S-(hydroxymethyl)mycothiol + NAD+
-
724589
Rhodococcus erythropolis
S-formylmycothiol + NADH + H+
-
-
-
?
S-(hydroxymethyl)mycothiol + NAD+
-
724589
Rhodococcus erythropolis N9T-4
S-formylmycothiol + NADH + H+
-
-
-
?
Temperature Optimum [°C]
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
30
-
assay at
Rhodococcus erythropolis
pH Optimum
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
7
-
assay at
Rhodococcus erythropolis
Cofactor
Cofactor
Commentary
Organism
Structure
NAD+
-
Rhodococcus erythropolis
Cloned(Commentary) (protein specific)
Commentary
Organism
mFADH genetic organization, overview
Rhodococcus erythropolis
Cofactor (protein specific)
Cofactor
Commentary
Organism
Structure
NAD+
-
Rhodococcus erythropolis
Natural Substrates/ Products (Substrates) (protein specific)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
S-(hydroxymethyl)mycothiol + NAD+
Rhodococcus erythropolis
-
S-formylmycothiol + NADH + H+
-
-
?
S-(hydroxymethyl)mycothiol + NAD+
Rhodococcus erythropolis N9T-4
-
S-formylmycothiol + NADH + H+
-
-
?
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
S-(hydroxymethyl)mycothiol + NAD+
-
724589
Rhodococcus erythropolis
S-formylmycothiol + NADH + H+
-
-
-
?
S-(hydroxymethyl)mycothiol + NAD+
-
724589
Rhodococcus erythropolis N9T-4
S-formylmycothiol + NADH + H+
-
-
-
?
Temperature Optimum [°C] (protein specific)
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
30
-
assay at
Rhodococcus erythropolis
pH Optimum (protein specific)
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
7
-
assay at
Rhodococcus erythropolis
Expression
Organism
Commentary
Expression
Rhodococcus erythropolis
the gene encoding the enzyme is not induced under oligotrophic conditions
additional information
Rhodococcus erythropolis
formaldehyde, added to the medium, induces the enzyme expression by 10fold
up
General Information
General Information
Commentary
Organism
metabolism
the organism has three distinct formaldehyde oxidation systems, the methlotrophhic oxidoreductase mycothiol-dependent formaldehyde dehydroganse is not a key enzyme invovled in oligotrophic growth
Rhodococcus erythropolis
General Information (protein specific)
General Information
Commentary
Organism
metabolism
the organism has three distinct formaldehyde oxidation systems, the methlotrophhic oxidoreductase mycothiol-dependent formaldehyde dehydroganse is not a key enzyme invovled in oligotrophic growth
Rhodococcus erythropolis
Expression (protein specific)
Organism
Commentary
Expression
Rhodococcus erythropolis
the gene encoding the enzyme is not induced under oligotrophic conditions
additional information
Rhodococcus erythropolis
formaldehyde, added to the medium, induces the enzyme expression by 10fold
up
Other publictions for EC 1.1.1.306
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
724589
Yoshida
Gene expression analysis of me ...
Rhodococcus erythropolis, Rhodococcus erythropolis N9T-4
Biosci. Biotechnol. Biochem.
75
123-127
2011
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1
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2
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3
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2
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1
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1
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1
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1
1
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2
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-
-
2
-
1
-
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-
1
-
-
-
2
1
1
2
-
-
690518
Lee
Thiols in nitric oxide synthas ...
Nocardia sp., Nocardia sp. NRRL 5646
Appl. Environ. Microbiol.
73
3095-3097
2007
1
1
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-
-
-
-
-
-
-
-
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-
6
-
-
-
-
-
-
-
-
2
-
-
-
-
-
-
-
-
1
-
-
-
1
1
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1
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-
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-
-
-
-
-
-
-
-
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-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
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654527
Vogt
The metabolism of nitrosothiol ...
Mycolicibacterium smegmatis, Mycolicibacterium smegmatis mc2
Biochem. J.
374
657-666
2003
-
-
-
-
-
-
-
2
-
-
1
-
-
5
-
-
1
-
-
1
1
-
4
1
-
-
-
1
1
-
-
1
-
-
-
-
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-
1
-
-
-
-
-
-
2
-
-
1
-
-
-
-
1
-
1
1
-
4
1
-
-
-
1
1
-
-
-
-
-
-
-
-
-
288322
Misset-Smits
Mycothiol, 1-O-(2'-[N-acwtyl-L ...
Amycolatopsis methanolica, Rhodococcus erythropolis
FEBS Lett.
409
221-222
1997
-
-
-
-
-
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1
-
-
-
-
2
-
5
-
-
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-
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2
1
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2
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-
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4
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4
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1
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2
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2
1
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2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
288323
Norin
Mycothiol-dependent formaldehy ...
Amycolatopsis methanolica
Eur. J. Biochem.
248
282-289
1997
-
-
-
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-
-
-
-
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1
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2
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1
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1
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1
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2
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2
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1
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1
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1
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1
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-
-
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288324
Van Ophem
NAD-linked, factor-dependent f ...
Amycolatopsis methanolica
Eur. J. Biochem.
206
511-518
1992
2
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9
8
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1
2
1
-
7
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1
-
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1
4
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3
1
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-
-
-
2
-
-
1
-
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-
2
-
-
1
-
-
-
-
9
-
8
-
1
2
1
-
-
-
1
-
1
4
-
3
1
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-
-
-
2
-
-
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