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Literature summary for 1.1.1.307 extracted from
- Induction of D-xylose uptake and expression of NAD(P)H-linked xylose reductase and NADP+-linked xylitol dehydrogenase in the oleaginous microalga Chlorella sorokiniana (2014), Biotechnol. Biofuels, 7, 125.
Application
| Application | Comment | Organism |
|---|---|---|
| synthesis | the microalga Chlorella sorokiniana and provide a target for genetic engineering to improve D-xylose utilization for microalgal lipid production | Chlorella sorokiniana |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| xylitol + NADP+ | Chlorella sorokiniana | - |
D-xylose + NADPH + H+ | - |
r |  |
| xylitol + NADP+ | Chlorella sorokiniana UTEX 1602 | - |
D-xylose + NADPH + H+ | - |
r | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Chlorella sorokiniana | - |
- |
- |
| Chlorella sorokiniana UTEX 1602 | - |
- |
- |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| cell culture | - |
Chlorella sorokiniana | - |
| additional information | D-xylose utilization by the oleaginous microalga Chlorella sorokiniana. Compared with the culture in the dark, the consumption of D-xylose increased 2fold under light | Chlorella sorokiniana | - |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 0.021 | - |
crude enzyme extract, induced cells, pH 6.0, 40°C | Chlorella sorokiniana |
| 0.027 | - |
crude enzyme extract, induced cells, pH 6.0, 70°C | Chlorella sorokiniana |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| D-xylose + NADH + H+ | - |
Chlorella sorokiniana | xylitol + NAD+ | - |
r | |
| D-xylose + NADH + H+ | - |
Chlorella sorokiniana UTEX 1602 | xylitol + NAD+ | - |
r | |
| D-xylose + NADPH + H+ | preferred substrates | Chlorella sorokiniana | xylitol + NADP+ | - |
r | |
| D-xylose + NADPH + H+ | preferred substrates | Chlorella sorokiniana UTEX 1602 | xylitol + NADP+ | - |
r | |
| xylitol + NADP+ | - |
Chlorella sorokiniana | D-xylose + NADPH + H+ | - |
r | |
| xylitol + NADP+ | - |
Chlorella sorokiniana UTEX 1602 | D-xylose + NADPH + H+ | - |
r | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| NAD(P)H-linked xylose reductase | - |
Chlorella sorokiniana |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 70 | - |
- |
Chlorella sorokiniana |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 6 | - |
- |
Chlorella sorokiniana |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 5.5 | 9 | about 90% of maximal activity at pH 5.5, 60% at pH 9.0, and maximal activity at pH 6.0 | Chlorella sorokiniana |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| additional information | the enzyme uses both NADPH and NADH but prefers NADPH, the activity with NADPH is about 5fold higher than that with NADH | Chlorella sorokiniana | |
| NAD+ | - |
Chlorella sorokiniana | |
| NADH | - |
Chlorella sorokiniana | |
| NADH | 5fold higher activity with NADPH than with NADH | Chlorella sorokiniana | |
| NADP+ | - |
Chlorella sorokiniana | |
| NADPH | - |
Chlorella sorokiniana | |
| NADPH | 5fold higher activity with NADPH than with NADH | Chlorella sorokiniana | |
Expression
| Organism | Comment | Expression |
|---|---|---|
| Chlorella sorokiniana | D-xylose uptake induces the enzyme, together with the NADP+-linked xylitol dehydrogenase, EC 1.1.1.10 | up |
| Chlorella sorokiniana | induction by presence of D-xylose in culture medium | up |
General Information
| General Information | Comment | Organism |
|---|---|---|
| metabolism | D-glucose-induced algal cells exhibit a remarkably increased D-xylose uptake rate. The uptake of D-xylose activates the related metabolic pathway, and the activities of a NAD(P)H-linked xylose reductase XR and NADP+-linked xylitol dehydrogenase XDH are detected in C. sorokiniana. Compared with the culture in the dark, the consumption of D-xylose increases 2fold under light but decreases to the same level with addition of 3-(3,4-dichlorophenyl)-1,1-dimethylurea | Chlorella sorokiniana |
| additional information | the transportation of D-xylose across the cell membrane of Chlorella sorokiniana is realized by an inducible hexose symporter. The uptake of D-xylose subsequently activates the expression of key catalytic enzymes that enables D-xylose entering central metabolism | Chlorella sorokiniana |
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Release 2023.1 (January 2023)
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