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Literature summary for 1.1.1.307 extracted from

  • Jo, J.H.; Park, Y.C.; Jin, Y.S.; Seo, J.H.
    Construction of efficient xylose-fermenting Saccharomyces cerevisiae through a synthetic isozyme system of xylose reductase from Scheffersomyces stipitis (2017), Biores. Technol., 241, 88-94 .
    View publication on PubMed
Search for more literature for 1.1.1.307

Cloned(Commentary)

Cloned (Comment) Organism
gene xyl1, the xylose-metabolic gene XYL1 from Scheffersomyces stipitis are introduced into Saccharomyces cerevisiae Scheffersomyces stipitis

Protein Variants

Protein Variants Comment Organism
additional information construction of efficient xylose-fermenting Saccharomyces cerevisiae strain DXS through a synthetic isozyme system of xylose reductase from Scheffersomyces stipitis. The xylose-metabolic genes XYL1, XYL2 (EC 1.1.1.9), and XYL3 (EC 2.7.1.17) from Scheffersomyces stipitis are introduced into Saccharomyces cerevisiae. Construction of control strains SR6 and MM through random integration of the XR (NADPH) or mXR (NADH) expression cassette, respectively, at the multipled elements of the genome of Saccharomyces cerevisiae strain D452-2. Fermentation parameters of engineered Saccharomyces cerevisiae strains in mixed sugar fermentations (glucose and xylose), overview Scheffersomyces stipitis

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
xylitol + NAD(P)+ Scheffersomyces stipitis
-
 D-xylose + NAD(P)H + H+
-
 r
xylitol + NAD(P)+ Scheffersomyces stipitis NRRL Y-11545
-
 D-xylose + NAD(P)H + H+
-
 r
xylitol + NAD(P)+ Scheffersomyces stipitis NBRC 10063
-
 D-xylose + NAD(P)H + H+
-
 r
xylitol + NAD(P)+ Scheffersomyces stipitis ATCC 58785
-
 D-xylose + NAD(P)H + H+
-
 r

Organism

Organism UniProt Comment Textmining
Scheffersomyces stipitis P31867
-
-
Scheffersomyces stipitis ATCC 58785 P31867
-
-
Scheffersomyces stipitis NBRC 10063 P31867
-
-
Scheffersomyces stipitis NRRL Y-11545 P31867
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
xylitol + NAD(P)+
-
 Scheffersomyces stipitis D-xylose + NAD(P)H + H+
-
 r
xylitol + NAD(P)+
-
 Scheffersomyces stipitis NRRL Y-11545 D-xylose + NAD(P)H + H+
-
 r
xylitol + NAD(P)+
-
 Scheffersomyces stipitis NBRC 10063 D-xylose + NAD(P)H + H+
-
 r
xylitol + NAD(P)+
-
 Scheffersomyces stipitis ATCC 58785 D-xylose + NAD(P)H + H+
-
 r

Synonyms

Synonyms Comment Organism
XYL1
-
 Scheffersomyces stipitis
xylose reductase
-
 Scheffersomyces stipitis

Cofactor

Cofactor Comment Organism Structure
NAD+
-
 Scheffersomyces stipitis
NADH
-
 Scheffersomyces stipitis
NADP+
-
 Scheffersomyces stipitis
NADPH
-
 Scheffersomyces stipitis

General Information

General Information Comment Organism
metabolism one xylose-assimilating pathway consists of xylose reductase (XR, XYL1) and xylitol dehydrogenase (XDH, XYL2, EC 1.1.1.9) from Scheffersomyces stipitis. XR reduces xylose to xylitol by using NAD(P)H as cofactor and XDH further oxidizes xylitol to xylulose using NAD+. While the XR-XDH pathway can offer higher metabolic fluxes than the xylose isomerase (XI) pathway, it accumulates xylitol which is produced due to cofactor imbalance caused by different cofactor requirement between XR and XDH Scheffersomyces stipitis