Application | Comment | Organism |
---|---|---|
synthesis | the enzyme can be used for industrial production of xylitol from waste xylose mother liquor. Production of xylitol from xylose mother liquor by immobilized Escherichia coli cells containing xylose reductase and glucose dehydrogenase, overview. The immobilization of Escherichia coli strain BL21(DE3)/ pCDFDuet-1-XR-GDH cells results in an increase of storage stability which can expand its potential applications in industries | Rhizopus arrhizus |
Cloned (Comment) | Organism |
---|---|
functional recombinant co-expression of xylose reductase (XR) from Rhizopus oryzae and glucose dehydrogenase (GDH) from Exiguobacterium sibiricum in Escherichia coli strain BL21(DE3) | Rhizopus arrhizus |
Protein Variants | Comment | Organism |
---|---|---|
additional information | cells of recombinant Escherichia coli strain BL21(DE3)/pCDFDuet-1-XR-GDH coexpressing xylose reductase (XR) and glucose dehydrogenase (GDH) are immobilized and applied for the production of xylitol from xylose mother liquor (XML). Various immobilization methods are screened and the cross-linking approach with diatomite and polyetherimide as the raw materials and glutaraldehyde as the cross-linking agent is the optimal one, and the recovery activity reached of 80.3% after immobilization. The half-life of immobilized cells is 1.52times to that of free cells. Batch experiments show that the enzyme activity of immobilized cells remains 70.5% of initial activity after 10 batches and the space-time yield of xylitol reaches of 11.5 g/l/h. Method development and optimization, overview | Rhizopus arrhizus |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
D-xylose + NADPH + H+ | Rhizopus arrhizus | - |
xylitol + NADP+ | - |
r |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Rhizopus arrhizus | W5RZ21 | - |
- |
Storage Stability | Organism |
---|---|
4°C, immobilized cells highly retain activity compared to free cells after storage for 20 days at 30°C. The immobilized cells maintain over 50% of their original activity after storage for 20 days at 4°C, while the catalytic activity of free cells decreases to approximately 50% after storage for 12 days at 4°C and to 30% after storage for 20 days at 4°C | Rhizopus arrhizus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
D-xylose + NADPH + H+ | - |
Rhizopus arrhizus | xylitol + NADP+ | - |
r |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
30 | - |
recombinant enzyme | Rhizopus arrhizus |
Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|
20 | 40 | over 60% of maximal activity, immobilized cells | Rhizopus arrhizus |
20 | 30 | over 60% of maximal activity, free cells | Rhizopus arrhizus |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8 | - |
recombinant enzyme | Rhizopus arrhizus |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
7 | 9 | over 80% of maximal activity, immobilized cells | Rhizopus arrhizus |
7 | 8 | over 80% of maximal activity, free cells | Rhizopus arrhizus |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NADP+ | - |
Rhizopus arrhizus | |
NADPH | - |
Rhizopus arrhizus |