Literature summary for 1.1.1.307 extracted from

Kim, H.; Lee, H.S.; Park, H.; Lee, D.H.; Boles, E.; Chung, D.; Park, Y.C.
Enhanced production of xylitol from xylose by expression of Bacillus subtilis arabinose H+ symporter and Scheffersomyces stipitis xylose reductase in recombinant Saccharomyces cerevisiae (2017), Enzyme Microb. Technol., 107, 7-14 .

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Cloned(Commentary)

Cloned (Comment)	Organism
gene, recombinant expression in Saccharomyces cerevisiae strains D452-2 or EBY.VW4000, coexpression with Bacillus subtilis arabinose:H+ symporter (AraE). AraE and XYL1 expression leads to 18fold higher xylitol production compared to control strain D452-2, AraE takes up xylose efficiently and gives the engineered Saccharomyces cerevisiae strain DXXA high xylitol productivity, while the yeast wild-type shows an inefficient transport of xylose. Method optimization, overview	Scheffersomyces stipitis

Cloned (Comment)

Organism

gene, recombinant expression in Saccharomyces cerevisiae strains D452-2 or EBY.VW4000, coexpression with Bacillus subtilis arabinose:H+ symporter (AraE). AraE and XYL1 expression leads to 18fold higher xylitol production compared to control strain D452-2, AraE takes up xylose efficiently and gives the engineered Saccharomyces cerevisiae strain DXXA high xylitol productivity, while the yeast wild-type shows an inefficient transport of xylose. Method optimization, overview

Scheffersomyces stipitis

Protein Variants

Protein Variants	Comment	Organism
additional information	enhanced production of xylitol from xylose by expression of Bacillus subtilis arabinose:H+ symporter and Scheffersomyces stipitis xylose reductase in recombinant Saccharomyces cerevisiae	Scheffersomyces stipitis

Natural Substrates/ Products (Substrates)

Natural Substrates	Organism	Comment (Nat. Sub.)	Natural Products	Comment (Nat. Pro.)	Rev.
D-xylose + NADPH + H+	Scheffersomyces stipitis	-	xylitol + NADP+	-	r
D-xylose + NADPH + H+	Scheffersomyces stipitis NRRL Y-11545	-	xylitol + NADP+	-	r
D-xylose + NADPH + H+	Scheffersomyces stipitis NBRC 10063	-	xylitol + NADP+	-	r
D-xylose + NADPH + H+	Scheffersomyces stipitis ATCC 58785	-	xylitol + NADP+	-	r

Organism

Organism	UniProt	Comment	Textmining
Scheffersomyces stipitis	P31867	-	-
Scheffersomyces stipitis ATCC 58785	P31867	-	-
Scheffersomyces stipitis NBRC 10063	P31867	-	-
Scheffersomyces stipitis NRRL Y-11545	P31867	-	-

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.
D-xylose + NADPH + H+	-	Scheffersomyces stipitis	xylitol + NADP+	-	r
D-xylose + NADPH + H+	-	Scheffersomyces stipitis NRRL Y-11545	xylitol + NADP+	-	r
D-xylose + NADPH + H+	-	Scheffersomyces stipitis NBRC 10063	xylitol + NADP+	-	r
D-xylose + NADPH + H+	-	Scheffersomyces stipitis ATCC 58785	xylitol + NADP+	-	r

Synonyms

Synonyms	Comment	Organism
NAD(P)H-dependent D-xylose reductase	UniProt	Scheffersomyces stipitis
XYL1	-	Scheffersomyces stipitis

Temperature Optimum [°C]

Temperature Optimum [°C]	Temperature Optimum Maximum [°C]	Comment	Organism
30	-	assay at	Scheffersomyces stipitis

pH Optimum

pH Optimum Minimum	pH Optimum Maximum	Comment	Organism
6	-	D-xylose reduction assay at	Scheffersomyces stipitis

Cofactor

Cofactor	Comment	Organism	Structure
NADP+	-	Scheffersomyces stipitis
NADPH	-	Scheffersomyces stipitis