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Literature summary for 1.1.1.309 extracted from

  • Blodgett, J.A.; Thomas, P.M.; Li, G.; Velasquez, J.E.; van der Donk, W.A.; Kelleher, N.L.; Metcalf, W.W.
    Unusual transformations in the biosynthesis of the antibiotic phosphinothricin tripeptide (2007), Nat. Chem. Biol., 3, 480-485.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

Cloned (Comment) Organism
overexpression of a histidine-tagged PhpC fusion protein in Streptomyces lividans Streptomyces viridochromogenes

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
phosphonoacetaldehyde + NADH + H+ Streptomyces viridochromogenes the enzyme catalyzes a step in the phosphinothricin tripeptide pathway 2-hydroxyethylphosphonate + NAD+
-
?

Organism

Organism UniProt Comment Textmining
Streptomyces viridochromogenes
-
DSM 40736
-

Purification (Commentary)

Purification (Comment) Organism
-
Streptomyces viridochromogenes

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
phosphonoacetaldehyde + NADH + H+ the enzyme catalyzes a step in the phosphinothricin tripeptide pathway Streptomyces viridochromogenes 2-hydroxyethylphosphonate + NAD+
-
?
phosphonoacetaldehyde + NADH + H+ the reaction proceeds to apparent completion in the presence of NADH, but to a lesser extent with NADPH, which suggests that NADH is the preferred cofactor Streptomyces viridochromogenes 2-hydroxyethylphosphonate + NAD+
-
?
phosphonoacetaldehyde + NADPH + H+ the reaction proceeds to apparent completion in the presence of NADH, but to a lesser extent with NADPH, which suggests that NADH is the preferred cofactor Streptomyces viridochromogenes 2-hydroxyethylphosphonate + NADP+
-
?

Synonyms

Synonyms Comment Organism
PhpC
-
Streptomyces viridochromogenes

Temperature Optimum [┬░C]

Temperature Optimum [┬░C] Temperature Optimum Maximum [┬░C] Comment Organism
30
-
assay at Streptomyces viridochromogenes

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.3
-
assay at Streptomyces viridochromogenes

Cofactor

Cofactor Comment Organism Structure
NADH the reaction proceeds to apparent completion in the presence of NADH, but to a lesser extent with NADPH, which suggests that NADH is the preferred cofactor Streptomyces viridochromogenes
NADPH the reaction proceeds to apparent completion in the presence of NADH, but to a lesser extent with NADPH, which suggests that NADH is the preferred cofactor Streptomyces viridochromogenes

General Information

General Information Comment Organism
malfunction the DELTAphpC deletion mutant retains the ability to produce phosphinothricin tripeptide Streptomyces viridochromogenes