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Literature summary for 1.1.1.309 extracted from

  • Shao, Z.; Blodgett, J.A.V.; Circello, B.T.; Eliot, A.C.; Woodyer, R.; Li, G.; van der Donk, W.A.; Metcalf, W.W.; Zhao, H.
    Biosynthesis of 2-hydroxyethylphosphonate, an unexpected intermediate common to multiple phosphonate biosynthetic pathways (2008), J. Biol. Chem., 283, 23161-23168.
    View publication on PubMedView publication on EuropePMC

Inhibitors

Inhibitors Comment Organism Structure
EDTA approximately 0.02-0.025 mM PhpC is incubated with 20-25 mM EDTA at 4Ā°C until the activity is completely abolished (usually 1-2 h) Streptomyces viridochromogenes

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.0193
-
NADH in 50 mM HEPES, 200 mM NaCl, pH 7.5, temperature not specified in the publication Streptomyces viridochromogenes
0.185
-
phosphonoacetaldehyde in 50 mM HEPES, 200 mM NaCl, pH 7.5, temperature not specified in the publication Streptomyces viridochromogenes

Metals/Ions

Metals/Ions Comment Organism Structure
Fe2+ the iron content of PhpC is less than 5% Streptomyces viridochromogenes
Zn2+ Zn2+ is required for enzymatic activity Streptomyces viridochromogenes

Organism

Organism UniProt Comment Textmining
Streptomyces viridochromogenes
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
Streptomyces viridochromogenes

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2-hydroxyethylphosphonate + NAD+ the catalytic efficiency of phosphonoacetaldehyde reduction is 377fold greater than that of the reverse reaction, and even at pH 9.0, the catalytic efficiency of the forward reaction is still 11fold greater than that of the reverse reaction Streptomyces viridochromogenes phosphonoacetaldehyde + NADH + H+
-
r
additional information no reaction is observed with hydroxymethylphosphonate and 3-hydroxypropylphosphonate Streptomyces viridochromogenes ?
-
?

Subunits

Subunits Comment Organism
monomer gel filtration Streptomyces viridochromogenes

Synonyms

Synonyms Comment Organism
PhpC
-
Streptomyces viridochromogenes

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.41
-
phosphonoacetaldehyde in 50 mM HEPES, 200 mM NaCl, pH 7.5, temperature not specified in the publication Streptomyces viridochromogenes

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7
-
phosphonoacetaldehyde reduction is more favorable under neutral conditions (optimal pH at 7.0) Streptomyces viridochromogenes
9
-
2-hydroxyethylphosphonate oxidation is favored under basic conditions (optimal pH at 9.0) Streptomyces viridochromogenes

Cofactor

Cofactor Comment Organism Structure
NADH PhpC is an NADH-dependent enzyme Streptomyces viridochromogenes

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
2.2
-
phosphonoacetaldehyde in 50 mM HEPES, 200 mM NaCl, pH 7.5, temperature not specified in the publication Streptomyces viridochromogenes