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Literature summary for 1.1.1.31 extracted from
- Cloning and overexpression of the 3-hydroxyisobutyrate dehydrogenase gene from Pseudomonas putida E23 (2003), Biosci. Biotechnol. Biochem., 67, 438-441.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
- |
Pseudomonas putida |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 30280 | - |
x * 30280, deduced from gene sequence, x * 30300, SDS-PAGE | Pseudomonas putida |
| 30300 | - |
x * 30280, deduced from gene sequence, x * 30300, SDS-PAGE | Pseudomonas putida |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Pseudomonas putida | Q76KL5 | recombinant enzyme expressed in Escherichia coli | - |
| Pseudomonas putida E23 | Q76KL5 | recombinant enzyme expressed in Escherichia coli | - |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Pseudomonas putida |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| L-3-hydroxyisobutyrate + NAD+ | high stereospecificity | Pseudomonas putida | 3-oxoisobutyrate + NADH | - |
r |  |
| L-3-hydroxyisobutyrate + NAD+ | high stereospecificity | Pseudomonas putida E23 | 3-oxoisobutyrate + NADH | - |
r | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| ? | x * 30280, deduced from gene sequence, x * 30300, SDS-PAGE | Pseudomonas putida |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| NAD+ | - |
Pseudomonas putida | |
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