Activating Compound | Comment | Organism | Structure |
---|---|---|---|
2-mercaptoethanol | about 182.8% activity at 1 mM | Pseudomonas denitrificans (nom. rej.) | |
dithiothreitol | about 184.8% activity at 1 mM | Pseudomonas denitrificans (nom. rej.) | |
EDTA | about 150.9% activity at 1 mM | Pseudomonas denitrificans (nom. rej.) |
Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli BL21(DE3) cells | Pseudomonas denitrificans (nom. rej.) |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
Ag+ | complete inhibition at 1 mM | Pseudomonas denitrificans (nom. rej.) | |
Co2+ | about 64.3% residual activity at 1 mM | Pseudomonas denitrificans (nom. rej.) | |
Cu2+ | about 14.5% residual activity at 1 mM | Pseudomonas denitrificans (nom. rej.) | |
Fe2+ | about 9.1% residual activity at 1 mM | Pseudomonas denitrificans (nom. rej.) | |
Hg2+ | complete inhibition at 1 mM | Pseudomonas denitrificans (nom. rej.) | |
Mg2+ | about 88.6% residual activity at 1 mM | Pseudomonas denitrificans (nom. rej.) | |
Ni2+ | about 62.3% residual activity at 1 mM | Pseudomonas denitrificans (nom. rej.) | |
Zn2+ | about 14.5% residual activity at 1 mM | Pseudomonas denitrificans (nom. rej.) |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.164 | - |
NAD+ | with 3-hydroxypropionic acid as cosubstrate, at 30°C and pH 9.0 | Pseudomonas denitrificans (nom. rej.) | |
0.178 | - |
(S)-3-hydroxyisobutyrate | at 30°C and pH 9.0 | Pseudomonas denitrificans (nom. rej.) | |
1.055 | - |
3-hydroxypropionic acid | at 30°C and pH 9.0 | Pseudomonas denitrificans (nom. rej.) | |
24.18 | - |
methyl DL-serine | at 30°C and pH 9.0 | Pseudomonas denitrificans (nom. rej.) | |
25.14 | - |
methyl (2S)-3-hydroxy-2-methylpropanoate | at 30°C and pH 9.0 | Pseudomonas denitrificans (nom. rej.) | |
117.5 | - |
L-serine | at 30°C and pH 9.0 | Pseudomonas denitrificans (nom. rej.) |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mn2+ | about 130.7% activity at 1 mM | Pseudomonas denitrificans (nom. rej.) | |
additional information | not influenced by Na+, K+, NH3+, and Li+ | Pseudomonas denitrificans (nom. rej.) |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
30000 | - |
x * 30000, SDS-PAGE | Pseudomonas denitrificans (nom. rej.) |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Pseudomonas denitrificans (nom. rej.) | - |
- |
- |
Pseudomonas denitrificans (nom. rej.) ATCC 13867 | - |
- |
- |
Purification (Comment) | Organism |
---|---|
Ni-NTA resin column chromatography | Pseudomonas denitrificans (nom. rej.) |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
(S)-3-hydroxyisobutyrate + NAD+ | the enzyme is very specific to (S)-3-hydroxyisobutyrate | Pseudomonas denitrificans (nom. rej.) | 2-methyl-3-oxopropanoate + NADH + H+ | - |
? | |
(S)-3-hydroxyisobutyrate + NAD+ | the enzyme is very specific to (S)-3-hydroxyisobutyrate | Pseudomonas denitrificans (nom. rej.) ATCC 13867 | 2-methyl-3-oxopropanoate + NADH + H+ | - |
? | |
3-hydroxypropionic acid + NAD+ | - |
Pseudomonas denitrificans (nom. rej.) | malonate semialdehyde + NADH + H+ | - |
r | |
3-hydroxypropionic acid + NAD+ | - |
Pseudomonas denitrificans (nom. rej.) ATCC 13867 | malonate semialdehyde + NADH + H+ | - |
r | |
L-serine + NAD+ | - |
Pseudomonas denitrificans (nom. rej.) | 3-oxo-L-alanine + NADH + H+ | - |
r | |
L-serine + NAD+ | - |
Pseudomonas denitrificans (nom. rej.) ATCC 13867 | 3-oxo-L-alanine + NADH + H+ | - |
r | |
methyl (2S)-3-hydroxy-2-methylpropanoate + NAD+ | - |
Pseudomonas denitrificans (nom. rej.) | methyl (2S)-2-methyl-3-oxopropanoate + NADH + H+ | - |
r | |
methyl (2S)-3-hydroxy-2-methylpropanoate + NAD+ | - |
Pseudomonas denitrificans (nom. rej.) ATCC 13867 | methyl (2S)-2-methyl-3-oxopropanoate + NADH + H+ | - |
r | |
methyl DL-serine + NAD+ | - |
Pseudomonas denitrificans (nom. rej.) | methyl 3-oxo-DL-alanine + NADH + H+ | - |
r | |
methyl DL-serine + NAD+ | - |
Pseudomonas denitrificans (nom. rej.) ATCC 13867 | methyl 3-oxo-DL-alanine + NADH + H+ | - |
r |
Subunits | Comment | Organism |
---|---|---|
? | x * 30000, SDS-PAGE | Pseudomonas denitrificans (nom. rej.) |
Synonyms | Comment | Organism |
---|---|---|
3HIBDH-IV | - |
Pseudomonas denitrificans (nom. rej.) |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
30 | - |
- |
Pseudomonas denitrificans (nom. rej.) |
Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|
25 | 40 | more than 80% activity between 25°C and 40°C | Pseudomonas denitrificans (nom. rej.) |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
6.62 | - |
methyl DL-serine | at 30°C and pH 9.0 | Pseudomonas denitrificans (nom. rej.) | |
6.717 | - |
NAD+ | with 3-hydroxypropionic acid as cosubstrate, at 30°C and pH 9.0 | Pseudomonas denitrificans (nom. rej.) | |
10.8 | - |
3-hydroxypropionic acid | at 30°C and pH 9.0 | Pseudomonas denitrificans (nom. rej.) | |
35.76 | - |
methyl (2S)-3-hydroxy-2-methylpropanoate | at 30°C and pH 9.0 | Pseudomonas denitrificans (nom. rej.) | |
47.55 | - |
L-serine | at 30°C and pH 9.0 | Pseudomonas denitrificans (nom. rej.) | |
80.79 | - |
(S)-3-hydroxyisobutyrate | at 30°C and pH 9.0 | Pseudomonas denitrificans (nom. rej.) |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
9 | - |
- |
Pseudomonas denitrificans (nom. rej.) |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
8 | 10 | more than 60% activity between pH 8.0 and 10.0 | Pseudomonas denitrificans (nom. rej.) |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NAD+ | dependent on | Pseudomonas denitrificans (nom. rej.) |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.275 | - |
methyl DL-serine | at 30°C and pH 9.0 | Pseudomonas denitrificans (nom. rej.) | |
0.404 | - |
L-serine | at 30°C and pH 9.0 | Pseudomonas denitrificans (nom. rej.) | |
1.433 | - |
methyl (2S)-3-hydroxy-2-methylpropanoate | at 30°C and pH 9.0 | Pseudomonas denitrificans (nom. rej.) | |
10.23 | - |
3-hydroxypropionic acid | at 30°C and pH 9.0 | Pseudomonas denitrificans (nom. rej.) | |
42.44 | - |
NAD+ | with 3-hydroxypropionic acid as cosubstrate, at 30°C and pH 9.0 | Pseudomonas denitrificans (nom. rej.) | |
464.9 | - |
(S)-3-hydroxyisobutyrate | at 30°C and pH 9.0 | Pseudomonas denitrificans (nom. rej.) |