Cloned (Comment) | Organism |
---|---|
overexpressed in Escherichia coli | Azoarcus sp. |
Crystallization (Comment) | Organism |
---|---|
sitting drop method in a buffer containing 12-18% PEG 8000, 0.05 M potassium phosphate, pH 7.5 and 3% 2-methyl-2,4-pentanediol. The structure of the apo-form of the enzyme is solved with a resolution at 2.1 A. The enzyme soaked with NAD+ and the inhibitor (R)-1-phenylethanol forms a different crystal form containing two subunits in the asymmetric unit | Azoarcus sp. |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
(R)-1-phenylethanol | 0.05 mM, 50% inhibition of acetophenone reduction activity | Azoarcus sp. |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
27000 | - |
4 * 27000 | Azoarcus sp. |
93000 | - |
gel filtration | Azoarcus sp. |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Azoarcus sp. | Q5P5I4 | - |
- |
Azoarcus sp. EbN1 | Q5P5I4 | - |
- |
Purification (Comment) | Organism |
---|---|
- |
Azoarcus sp. |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
(S)-1-phenylethanol + NAD+ | - |
Azoarcus sp. | acetophenone + NADH + H+ | - |
r | |
(S)-1-phenylethanol + NAD+ | - |
Azoarcus sp. EbN1 | acetophenone + NADH + H+ | - |
r |
Subunits | Comment | Organism |
---|---|---|
tetramer | 4 * 27000 | Azoarcus sp. |
Synonyms | Comment | Organism |
---|---|---|
PED | - |
Azoarcus sp. |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
25 | - |
assay at | Azoarcus sp. |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.8 | - |
assay at | Azoarcus sp. |