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Literature summary for 1.1.1.312 extracted from

  • Lin, C.Y.; Vuu, K.M.; Amer, B.; Shih, P.M.; Baidoo, E.E.K.; Scheller, H.V.; Eudes, A.
    In-planta production of the biodegradable polyester precursor 2-pyrone-4,6-dicarboxylic acid (PDC) stacking reduced biomass recalcitrance with value-added co-product (2021), Metab. Eng., 66, 148-156 .
    View publication on PubMed
Search for more literature for 1.1.1.312

Application

Application Comment Organism
biotechnology engineering plants with the proposed de-novo PDC pathway provides an avenue to enrich biomass with a value-added co-product while simultaneously improving biomass quality for the supply of fermentable sugars. Implementing this strategy into bioenergy crops has the potential to support existing microbial fermentation approaches that exploit lignocellulosic biomass feedstocks for PDC production Comamonas testosteroni

Cloned(Commentary)

Cloned (Comment) Organism
gene pmdC, transient recombinant expression of the enzyme in Nicotiana benthamiana, coexpression with PmdA and, PmdB from Comamonas testosteroni, stable expression of enzymes PmdA, PmdB, and PmdC from Comamonas testosteroni in Arabidopsis thaliana, coexpression with 3-dehydroshikimate dehydratase (QsuB). The metabolic pathway is implemented in the Arabidopsis thaliana ecotype Columbia plastids to produce 2-pyrone-4,6-dicarboxylic acid (PDC), overview Comamonas testosteroni

Protein Variants

Protein Variants Comment Organism
additional information in tobacco leaves, transient expression of bacterial feedback-resistant 3-deoxy-D-arabinoheptulosonate 7-phosphate synthase (AroG) and 3-dehydroshikimate dehydratase (QsuB) produces high titers of protocatechuate (PCA), which is in turn efficiently converted into 2-pyrone-4,6-dicarboxylic acid (PDC) upon co-expression of PCA 4,5-dioxygenase (PmdAB) and 4-carboxy-2-hydroxymuconate-6-semialdehyde dehydrogenase (PmdC) derived from Comamonas testosteroni. Stable expression of AroG in Arabidopsis thaliana in a genetic background containing the QsuB gene enhances PCA content in plant biomass, presumably via an increase of the carbon flux through the shikimate pathway. Introducing AroG and the PDC biosynthetic genes (PmdA, PmdB, and PmdC) into the Arabidopsis QsuB background, or introducing the five genes (AroG, QsuB, PmdA, PmdB, and PmdC) stacked on a single construct into wild-type plants, results in PDC titers of about 1% and about 3% dry weight in plant biomass, respectively. Consistent with previous studies of plants expressing QsuB, all PDC producing lines show strong reduction in lignin content in stems. This low lignin trait is accompanied with improvements of biomass saccharification efficiency due to reduced cell wall recalcitrance to enzymatic degradation. Importantly, most transgenic lines show no reduction in biomass yields Comamonas testosteroni

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
4-carboxy-2-hydroxymuconate semialdehyde hemiacetal + NADP+ Comamonas testosteroni
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 2-oxo-2H-pyran-4,6-dicarboxylate + NADPH + H+
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 ?

Organism

Organism UniProt Comment Textmining
Comamonas testosteroni Q93PS4
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-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4-carboxy-2-hydroxymuconate semialdehyde hemiacetal + NADP+
-
 Comamonas testosteroni 2-oxo-2H-pyran-4,6-dicarboxylate + NADPH + H+
-
 ?

Synonyms

Synonyms Comment Organism
4-carboxy-2-hydroxymuconate-6-semialdehyde dehydrogenase
-
 Comamonas testosteroni
PmdC
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 Comamonas testosteroni

Cofactor

Cofactor Comment Organism Structure
NADP+
-
 Comamonas testosteroni