Cloned (Comment) | Organism |
---|---|
DNA and amino acid sequence determination and analysis, phylogenetic analysis, expression in GST-tagged Escherichia coli strain BL21 | Larrea tridentata |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.21 | - |
4-coumaryl acetate | pH 7.5, 30°C, recombinant enzyme | Larrea tridentata | |
0.29 | - |
coniferyl acetate | pH 7.5, 30°C, recombinant enzyme | Larrea tridentata |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
4-coumaryl acetate + NADPH + H+ | Larrea tridentata | - |
chavicol + acetate + NADP+ | - |
? | |
coniferyl acetate + NADPH + H+ | Larrea tridentata | - |
eugenol + acetate + NADP+ | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Larrea tridentata | - |
- |
- |
Purification (Comment) | Organism |
---|---|
recombinant GST-taged enzyme from Escherichia coli strain BL21 by glutathione affinity chromatography and dialysis | Larrea tridentata |
Reaction | Comment | Organism | Reaction ID |
---|---|---|---|
eugenol + a carboxylate + NADP+ = a coniferyl ester + NADPH + H+ | reaction mechanism, detailed overview | Larrea tridentata |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
leaf | - |
Larrea tridentata | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
4-coumaryl acetate + NADPH + H+ | - |
Larrea tridentata | chavicol + acetate + NADP+ | - |
? | |
4-coumaryl acetate + NADPH + H+ | - |
Larrea tridentata | chavicol + acetate + NADP+ | GC-MS analysis product analysis | ? | |
coniferyl acetate + NADPH + H+ | - |
Larrea tridentata | eugenol + acetate + NADP+ | - |
? | |
coniferyl acetate + NADPH + H+ | - |
Larrea tridentata | eugenol + acetate + NADP+ | GC-MS analysis product analysis | ? |
Synonyms | Comment | Organism |
---|---|---|
chavicol/eugenol synthase | - |
Larrea tridentata |
LtCES1 | - |
Larrea tridentata |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
30 | - |
assay at | Larrea tridentata |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
6.46 | - |
coniferyl acetate | pH 7.5, 30°C, recombinant enzyme | Larrea tridentata | |
6.8 | - |
4-coumaryl acetate | pH 7.5, 30°C, recombinant enzyme | Larrea tridentata |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.5 | - |
assay at | Larrea tridentata |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NADPH | - |
Larrea tridentata |
General Information | Comment | Organism |
---|---|---|
additional information | the enzyme is a PIP reductase homologue | Larrea tridentata |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
19500 | - |
4-coumaryl acetate | pH 7.5, 30°C, recombinant enzyme | Larrea tridentata | |
22000 | - |
coniferyl acetate | pH 7.5, 30°C, recombinant enzyme | Larrea tridentata |