Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 1.1.1.332 extracted from

  • Wallwey, C.; Matuschek, M.; Xie, X.; Li, S.
    Ergot alkaloid biosynthesis in Aspergillus fumigatus: Conversion of chanoclavine-I aldehyde to festuclavine by the festuclavine synthase FgaFS in the presence of the old yellow enzyme FgaOx3 (2010), Org. Biomol. Chem., 8, 3500-3508.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
expression in Escherichia coli Aspergillus fumigatus

Inhibitors

Inhibitors Comment Organism Structure
EDTA 485 residual activity Aspergillus fumigatus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.27
-
chanoclavine-I pH 7.5, 37┬░C Aspergillus fumigatus
1.1
-
NAD+ pH 7.5, 37┬░C Aspergillus fumigatus

Metals/Ions

Metals/Ions Comment Organism Structure
Mn2+ addition to EDTA-treated enzyme restores activity to 73% of initial activity Aspergillus fumigatus

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
30000
-
4* 27800, calculated, 4 * 30000, SDS-PAGE of His-tagged recombinant protein Aspergillus fumigatus
118000
-
gel filtration Aspergillus fumigatus

Organism

Organism UniProt Comment Textmining
Aspergillus fumigatus D3J0Z1
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
chanoclavine-I + NAD+ incubation of enzyme with chanoclavine-I aldehyde in the presence of NADH does not result in detectable chanoclavine-I, indicating a much lower reaction velocity of the reverse reaction Aspergillus fumigatus chanoclavine-I aldehyde + NADH + H+
-
?

Subunits

Subunits Comment Organism
tetramer 4* 27800, calculated, 4 * 30000, SDS-PAGE of His-tagged recombinant protein Aspergillus fumigatus

Synonyms

Synonyms Comment Organism
fgaDH
-
Aspergillus fumigatus

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.38
-
chanoclavine-I pH 7.5, 37┬░C Aspergillus fumigatus

Cofactor

Cofactor Comment Organism Structure
additional information no cofactors: NADP+, FAD, FMN Aspergillus fumigatus
NAD+ specific for NAD+ Aspergillus fumigatus