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Literature summary for 1.1.1.332 extracted from
- A bifunctional old yellow enzyme from Penicillium roqueforti is involved in ergot alkaloid biosynthesis (2017), Org. Biomol. Chem., 15, 8059-8071 .
Activating Compound
| Activating Compound | Comment | Organism | Structure |
|---|---|---|---|
| FgaOx3Pr3 | an old yellow enzyme (OYE), does not convert chanoclavine-I aldehyde to its shunt products in the absence of FgaFS. Instead, it increases significantly the product yields of several SDRs for the conversion of chanoclavine-I to its aldehyde. Kinetic studies prove that overcoming the product inhibition is responsible for the observed enhancement. Bifunctionality of OYE and synergistic effect with SDRs | Penicillium roqueforti |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.082 | - |
NAD+ | pH not specified in the publication, 30°C | Penicillium roqueforti |  |
| 0.573 | - |
chanoclavine-I | pH not specified in the publication, 30°C | Penicillium roqueforti | |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| chanoclavine-I + NAD+ | Penicillium roqueforti | - |
chanoclavine-I aldehyde + NADH + H+ | - |
r | |
| chanoclavine-I + NAD+ | Penicillium roqueforti FM164 | - |
chanoclavine-I aldehyde + NADH + H+ | - |
r | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Penicillium roqueforti | - |
- |
- |
| Penicillium roqueforti FM164 | - |
- |
- |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| chanoclavine-I + NAD+ | - |
Penicillium roqueforti | chanoclavine-I aldehyde + NADH + H+ | - |
r | |
| chanoclavine-I + NAD+ | - |
Penicillium roqueforti FM164 | chanoclavine-I aldehyde + NADH + H+ | - |
r | |
| additional information | LC-MS analysis of the in vitro assays | Penicillium roqueforti | ? | - |
- |
|
| additional information | LC-MS analysis of the in vitro assays | Penicillium roqueforti FM164 | ? | - |
- |
|
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| ChaDH | - |
Penicillium roqueforti |
| FgaDHPr | - |
Penicillium roqueforti |
| ifgE | - |
Penicillium roqueforti |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 30 | - |
assay at | Penicillium roqueforti |
Turnover Number [1/s]
| Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.135 | - |
NAD+ | pH not specified in the publication, 30°C | Penicillium roqueforti | |
| 0.172 | - |
chanoclavine-I | pH not specified in the publication, 30°C | Penicillium roqueforti | |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| NAD+ | - |
Penicillium roqueforti | |
| NADH | - |
Penicillium roqueforti | |
General Information
| General Information | Comment | Organism |
|---|---|---|
| evolution | the enzyme belongs to the short-chain dehydrogenase/reductase (SDR) family | Penicillium roqueforti |
| metabolism | the enzyme is involved in the biosynthesis of festuclavine from dimethylallylpyrosphosphate and tryptophane, the ergot alkaloid pathway | Penicillium roqueforti |
kcat/KM [mM/s]
| kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.3 | - |
chanoclavine-I | pH not specified in the publication, 30°C | Penicillium roqueforti | |
| 1.65 | - |
NAD+ | pH not specified in the publication, 30°C | Penicillium roqueforti | |
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