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Literature summary for 1.1.1.334 extracted from

  • Jirschitzka, J.; Schmidt, G.W.; Reichelt, M.; Schneider, B.; Gershenzon, J.; D'Auria, J.C.
    Plant tropane alkaloid biosynthesis evolved independently in the Solanaceae and Erythroxylaceae (2012), Proc. Natl. Acad. Sci. USA, 109, 10304-10309.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli Erythroxylum coca

Inhibitors

Inhibitors Comment Organism Structure
Co2+ 5 mM, inhibition by less than 30% Erythroxylum coca
Cu2+ 5 mM, 8% residual activity Erythroxylum coca
Fe2+ 5 mM, inhibition by less than 30% Erythroxylum coca
methyl ecgonine
-
Erythroxylum coca
methyl ecgonone
-
Erythroxylum coca
NADP+
-
Erythroxylum coca
NADPH
-
Erythroxylum coca
Zn2+ 5 mM, 58% residual activity Erythroxylum coca

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.0009
-
NADPH pH 6.8, temperature not specified in the publication Erythroxylum coca
0.0009
-
NADP+ pH 6.8, temperature not specified in the publication Erythroxylum coca
0.069
-
methyl ecgonone pH 6.8, temperature not specified in the publication Erythroxylum coca
0.151
-
methyl ecgonine pH 6.8, temperature not specified in the publication Erythroxylum coca

Metals/Ions

Metals/Ions Comment Organism Structure
additional information not activated by monovalent or divalent cations Erythroxylum coca

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
36910
-
calculated from the amino acid sequence Erythroxylum coca

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
methyl ecgonone + NADPH + H+ Erythroxylum coca cocaine biosynthesis methyl ecgonine + NADP+
-
r

Organism

Organism UniProt Comment Textmining
Erythroxylum coca E7C196
-
-

Purification (Commentary)

Purification (Comment) Organism
-
Erythroxylum coca

Source Tissue

Source Tissue Comment Organism Textmining
flower less than 20% of activity compared to young leaves Erythroxylum coca
-
leaf highest activity in young leaves Erythroxylum coca
-
mesophyll of young leaves Erythroxylum coca
-
additional information no activity in mature leaves or in roots Erythroxylum coca
-
seed
-
Erythroxylum coca
-
stem less than 20% of activity compared to young leaves Erythroxylum coca
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [┬Ámol/min/mg] Specific Activity Maximum [┬Ámol/min/mg] Comment Organism
0.0002669
-
pH 6.8 temperature not specified in the publication Erythroxylum coca

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
6-hydroxytropinone + NADPH + H+ reduction at 45% of the activity with methylecgonone Erythroxylum coca ? + NADP+
-
r
methyl ecgonone + NADH + H+ NADH can substitute for NADPH with 14% of the activity compared to NADPH Erythroxylum coca methyl ecgonine + NAD+
-
r
methyl ecgonone + NADPH + H+
-
Erythroxylum coca methyl ecgonine + NADP+
-
r
methyl ecgonone + NADPH + H+ cocaine biosynthesis Erythroxylum coca methyl ecgonine + NADP+
-
r
additional information no reduction of 8-thiabicyclo[3.2.1]octan-3-one, cyclohexanone, cyclooctanone, N-methyl-4-piperidone Erythroxylum coca ?
-
?
nortropinone + NADPH + H+ reduction at 6% of the activity with methylecgonone Erythroxylum coca ? + NADP+
-
r
tropinone + NADPH + H+ reduction at 36% of the activity with methylecgonone Erythroxylum coca pseudotropine + NADP+
-
r

Synonyms

Synonyms Comment Organism
MecgoR
-
Erythroxylum coca

Temperature Stability [┬░C]

Temperature Stability Minimum [┬░C] Temperature Stability Maximum [┬░C] Comment Organism
25
-
30 min, more than 30% loss of activity Erythroxylum coca
37
-
30 min, more than 70% loss of activity Erythroxylum coca
50
-
30 min, complete inactivation abvoe 50┬░C Erythroxylum coca

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
6.8
-
reduction of methyl ecgonone Erythroxylum coca
9.8
-
oxidation of methyl ecgonine Erythroxylum coca

Cofactor

Cofactor Comment Organism Structure
NADH 14% of the activity with NADPH Erythroxylum coca
NADPH
-
Erythroxylum coca

Ki Value [mM]

Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
0.001136
-
methyl ecgonone substrate inhibition, pH 6.8, temperature not specified in the publication Erythroxylum coca
0.00248
-
NADPH substrate inhibition, pH 6.8, temperature not specified in the publication Erythroxylum coca
0.002673
-
methyl ecgonine substrate inhibition, pH 6.8, temperature not specified in the publication Erythroxylum coca
0.506
-
NADP+ substrate inhibition, pH 6.8, temperature not specified in the publication Erythroxylum coca