Cloned (Comment) | Organism |
---|---|
expression in Escherichia coli | Bordetella pertussis |
expression in Escherichia coli | Chromobacterium violaceum |
Crystallization (Comment) | Organism |
---|---|
in presence of NAD(H) and substrate to 2.13 A and 1.5 A resolution. Enzyme displays octameric quaternary structure with the active sites positioned far apart. The octamers can be envisioned as tetramers of dimers. The carboxylate group attached to the C-5' carbon of the hexose in the natural substrate, UDP-N-acetyl-D-glucosaminuronic acid, is held firmly in place in the enzyme WlbA active site by the side chains of Arg165 and Tyr169 | Bordetella pertussis |
in presence of NAD(H) and substrate to 2.13 A and 1.5 A resolution. Enzyme displays octameric quaternary structure with the active sites positioned far apart. The octamers can be envisioned as tetramers of dimers. The carboxylate group attached to the C-5' carbon of the hexose in the natural substrate, UDP-N-acetyl-D-glucosaminuronic acid, is held firmly in place in the enzyme WlbA active site by the side chains of Arg165 and Tyr169 | Chromobacterium violaceum |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0056 | - |
UDP-2-acetamido-2-deoxy-alpha-D-glucuronate | presence of transaminase WlbC, 25°C, pH 8.5 | Chromobacterium violaceum | |
0.015 | - |
UDP-2-acetamido-2-deoxy-alpha-D-glucuronate | presence of transaminase WlbC, 25°C, pH 8.5 | Bordetella pertussis | |
0.2 | - |
UDP-alpha-D-glucuronate | 25°C, pH 8.5 | Chromobacterium violaceum | |
0.47 | - |
NAD+ | 25°C, pH 8.5 | Chromobacterium violaceum | |
0.97 | - |
NAD+ | 25°C, pH 8.5 | Bordetella pertussis | |
1.1 | - |
UDP-2-acetamido-2-deoxy-alpha-D-glucuronate | 25°C, pH 8.5 | Chromobacterium violaceum | |
2.05 | - |
UDP-alpha-D-glucuronate | 25°C, pH 8.5 | Bordetella pertussis | |
2.36 | - |
UDP-2-acetamido-2-deoxy-alpha-D-glucuronate | 25°C, pH 8.5 | Bordetella pertussis |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Bordetella pertussis | - |
- |
- |
Chromobacterium violaceum | Q7NQL0 | putative | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | the reaction mechanism is sequential, enzyme does not require 2-ketoglutarate for activity. No substrates: UDP-glucose, UDP-galactose, UDP-N-acetylglucosamine | Bordetella pertussis | ? | - |
? | |
additional information | the reaction mechanism is sequential, enzyme does not require 2-oxoglutarate for activity. No substrates: UDP-glucose, UDP-galactose, UDP-N-acetylglucosamine | Chromobacterium violaceum | ? | - |
? | |
UDP-2-acetamido-2-deoxy-alpha-D-glucuronate + NAD+ | - |
Bordetella pertussis | UDP-2-acetamido-2-deoxy-alpha-D-ribo-hex-3-uluronate + NADH + H+ | - |
? | |
UDP-2-acetamido-2-deoxy-alpha-D-glucuronate + NAD+ | - |
Chromobacterium violaceum | UDP-2-acetamido-2-deoxy-alpha-D-ribo-hex-3-uluronate + NADH + H+ | - |
? | |
UDP-alpha-D-glucuronate + NAD+ | - |
Bordetella pertussis | UDP-alpha-D-ribo-hex-3-uluronate + NADH + H+ | - |
? | |
UDP-alpha-D-glucuronate + NAD+ | - |
Chromobacterium violaceum | UDP-alpha-D-ribo-hex-3-uluronate + NADH + H+ | - |
? |
Synonyms | Comment | Organism |
---|---|---|
WlbA | - |
Bordetella pertussis |
WlbA | - |
Chromobacterium violaceum |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.00015 | - |
UDP-alpha-D-glucuronate | 25°C, pH 8.5 | Chromobacterium violaceum | |
0.00031 | - |
UDP-2-acetamido-2-deoxy-alpha-D-glucuronate | 25°C, pH 8.5 | Chromobacterium violaceum | |
0.00076 | - |
UDP-2-acetamido-2-deoxy-alpha-D-glucuronate | presence of transaminase WlbC, 25°C, pH 8.5 | Chromobacterium violaceum | |
0.0021 | - |
UDP-alpha-D-glucuronate | 25°C, pH 8.5 | Bordetella pertussis | |
0.0039 | - |
UDP-2-acetamido-2-deoxy-alpha-D-glucuronate | presence of transaminase WlbC, 25°C, pH 8.5 | Bordetella pertussis | |
0.0047 | - |
UDP-2-acetamido-2-deoxy-alpha-D-glucuronate | 25°C, pH 8.5 | Bordetella pertussis |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NAD+ | - |
Bordetella pertussis | |
NAD+ | - |
Chromobacterium violaceum |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.00028 | - |
UDP-2-acetamido-2-deoxy-alpha-D-glucuronate | 25°C, pH 8.5 | Chromobacterium violaceum | |
0.00075 | - |
UDP-alpha-D-glucuronate | 25°C, pH 8.5 | Chromobacterium violaceum | |
0.001 | - |
UDP-alpha-D-glucuronate | 25°C, pH 8.5 | Bordetella pertussis | |
0.002 | - |
UDP-2-acetamido-2-deoxy-alpha-D-glucuronate | 25°C, pH 8.5 | Bordetella pertussis | |
0.136 | - |
UDP-2-acetamido-2-deoxy-alpha-D-glucuronate | presence of transaminase WlbC, 25°C, pH 8.5 | Chromobacterium violaceum | |
0.26 | - |
UDP-2-acetamido-2-deoxy-alpha-D-glucuronate | presence of transaminase WlbC, 25°C, pH 8.5 | Bordetella pertussis |