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Literature summary for 1.1.1.335 extracted from

  • Shoji, M.; Sato, K.; Yukitake, H.; Naito, M.; Nakayama, K.
    Involvement of the Wbp pathway in the biosynthesis of Porphyromonas gingivalis lipopolysaccharide with anionic polysaccharide (2014), Sci. Rep., 4, 5056 .
    View publication on PubMedView publication on EuropePMC
Search for more literature for 1.1.1.335

Cloned(Commentary)

Cloned (Comment) Organism
gene wbpB or PGN_0168, from mutant strain HG66, DNA and amino acid sequence determination and analysis, complementation of the wbpB-defective mutant strain by expression of wild-type gene wbpB Porphyromonas gingivalis

Protein Variants

Protein Variants Comment Organism
additional information detection nonsense mutation in the gene wbpB from strain HG66 by sequencing. Porphyromonas gingivalis strain HG66 lacks lipopolysaccharide A-LPS. Introduction of a wild-type wbpB gene into strain HG66 restores formation of A-LPS. Presence of a nonsense mutation in the gene, the porR mutation. The porR mutant possesses O-LPS, but lacks A-LPS Porphyromonas gingivalis

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
UDP-N-acetyl-alpha-D-mannosamine + 2 NAD+ + H2O Porphyromonas gingivalis
-
 UDP-N-acetyl-alpha-D-mannosaminuronate + 2 NADH + 2 H+
-
 ?
UDP-N-acetyl-alpha-D-mannosamine + 2 NAD+ + H2O Porphyromonas gingivalis DSM 20709
-
 UDP-N-acetyl-alpha-D-mannosaminuronate + 2 NADH + 2 H+
-
 ?

Organism

Organism UniProt Comment Textmining
Porphyromonas gingivalis B2RH42
-
-
Porphyromonas gingivalis DSM 20709 B2RH42
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
UDP-N-acetyl-alpha-D-mannosamine + 2 NAD+ + H2O
-
 Porphyromonas gingivalis UDP-N-acetyl-alpha-D-mannosaminuronate + 2 NADH + 2 H+
-
 ?
UDP-N-acetyl-alpha-D-mannosamine + 2 NAD+ + H2O
-
 Porphyromonas gingivalis DSM 20709 UDP-N-acetyl-alpha-D-mannosaminuronate + 2 NADH + 2 H+
-
 ?

Synonyms

Synonyms Comment Organism
PGN_0168
-
 Porphyromonas gingivalis
WbpB
-
 Porphyromonas gingivalis

Cofactor

Cofactor Comment Organism Structure
NAD+
-
 Porphyromonas gingivalis

General Information

General Information Comment Organism
malfunction mutants porR and porT are involved in the pigmentation phenotype in Porphyromonas gingivalis. Porphyromonas gingivalis has two different lipopolysaccharide (LPS) molecules, O-LPS and A-LPS. Porphyromonas gingivalis strain HG66 lacks lipopolysaccharide A-LPS. Introduction of a wild-type wbpB gene into strain HG66 restores formation of A-LPS. Sequencing of the wbpB gene from strain HG66 has revealed the presence of a nonsense mutation in the gene, the porR mutation. The porR mutant possesses O-LPS, but lacks A-LPS Porphyromonas gingivalis
physiological function A-LPS consists of a lipid A-core and anionic polysaccharide (APS) repeating units containing phosphorylated branched mannan. The PorR, WbpB and UgdA proteins are predicted to participate in the initial synthesis of structural sugar(s) in the anionic polysaccharide Porphyromonas gingivalis