Crystallization (Comment) | Organism |
---|---|
in complex with NADH, to 2.5 A resolution. The asymmetric unit contains a tetramer of tight dimers. Structure does not contain a cofactor-binding domain with Rossmann-fold topology. The NADH is bound in an extended conformation in each active site, in a manner that explains the pro-S specificity. Cofactor binding involves residues belonging to both subunits within the tight dimers. The results indicate the existence of a substrate discrimination mechanism, which involves electrostatic interactions | Methanocaldococcus jannaschii |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.04 | - |
3-sulfopyruvate | pH 8.0, 70°C | Methanocaldococcus jannaschii | |
0.13 | - |
oxaloacetate | pH 8.0, 70°C | Methanocaldococcus jannaschii | |
1.9 | - |
2-oxopentanedioic acid | pH 8.0, 70°C | Methanocaldococcus jannaschii | |
3.4 | - |
oxopropandioic acid | pH 8.0, 70°C | Methanocaldococcus jannaschii | |
46 | - |
glyoxylate | pH 8.0, 70°C | Methanocaldococcus jannaschii |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
75000 | - |
2 * 75000, calculated | Methanocaldococcus jannaschii |
150000 | - |
gel filtration and velocity sedimentation data | Methanocaldococcus jannaschii |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Methanocaldococcus jannaschii | Q58820 | - |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
2-oxopentanedioic acid + NADH + H+ | - |
Methanocaldococcus jannaschii | 2-hydroxypentandioic acid + NAD+ | - |
? | |
3-sulfopyruvate + NADH + H+ | - |
Methanocaldococcus jannaschii | 3-sulfolactate + NAD+ | - |
? | |
glyoxylate + NADH + H+ | - |
Methanocaldococcus jannaschii | 2-hydroxypropanoate + NAD+ | - |
? | |
additional information | no substrates: pyruvate, 2-oxobutanoate, 2-oxovalerate, ketoisovalerate, 2-oxohexanedioate, 2-ketohexanedioate, 3-methyl 2-oxovalerate and 4-methyl-2-oxovalerate | Methanocaldococcus jannaschii | ? | - |
? | |
oxaloacetate + NADH + H+ | - |
Methanocaldococcus jannaschii | malate + NAD+ | - |
? | |
oxopropandioic acid + NADH + H+ | - |
Methanocaldococcus jannaschii | 2-hydroxypropanedioic acid + NAD+ | - |
? |
Subunits | Comment | Organism |
---|---|---|
dimer | 2 * 75000, calculated | Methanocaldococcus jannaschii |