Cloned (Comment) | Organism |
---|---|
coexpression of the Sulfolobus solfataricus hmgA gene in Escherichia coli with the argU gene that encodes tRNAAGA,AGG results in an over 10fold increase in enzyme yield | Saccharolobus solfataricus |
expression in Escherichia coli | Saccharolobus solfataricus |
Protein Variants | Comment | Organism |
---|---|---|
synthesis | due to use of rare codon, expression of enzyme in Escherichia coli is poor. Coexpression of the S. solfataricus hmgA gene with the argU gene that encodes tRNAAGA,AGG resulted in an over 10-fold increase in enzyme yield | Saccharolobus solfataricus |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
Mevinolin | competitive with 3-hydroxy-3-methylglutaryl-CoA | Saccharolobus solfataricus |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
3-hydroxy-3-methylglutaryl-CoA + NADPH | Saccharolobus solfataricus | - |
(R)-mevalonate + CoA + NADP+ | - |
r |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Saccharolobus solfataricus | - |
- |
- |
Purification (Comment) | Organism |
---|---|
- |
Saccharolobus solfataricus |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
4.9 | - |
pH 5.5, 50°C | Saccharolobus solfataricus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
3-hydroxy-3-methylglutaryl-CoA + NADPH | - |
Saccharolobus solfataricus | (R)-mevalonate + CoA + NADP+ | - |
? | |
3-hydroxy-3-methylglutaryl-CoA + NADPH | - |
Saccharolobus solfataricus | (R)-mevalonate + CoA + NADP+ | - |
r |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
5.5 | - |
- |
Saccharolobus solfataricus |
9.5 | - |
oxidative acylation of (R,S)-mevaldehyde or mevalonate | Saccharolobus solfataricus |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NADPH | - |
Saccharolobus solfataricus |
Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|
0.000005 | - |
Mevinolin | pH 5.5, 50°C | Saccharolobus solfataricus |