Any feedback?
Literature summary for 1.1.1.34 extracted from
- Characterization and function of the 3-hydroxy-3-methylglutaryl-CoA reductase gene in Alisma orientale (Sam.) Juz. and its relationship with protostane triterpene production (2015), Plant Physiol. Biochem., 97, 378-389.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| gene HMGR, DNA and amino acid sequence determination and analysis, sequence comparisons and phylogenetic analysis and tree, recombinant expression in Escherichia coli strain BL21(DE3) Rosetta, quantitative realtime-PCR enzyme expression analysis | Alisma plantago-aquatica subsp. orientale |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| Alisol B 23-actetate | - |
Alisma plantago-aquatica subsp. orientale |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 64600 | - |
- |
Alisma plantago-aquatica subsp. orientale |
| 66500 | - |
- |
Alisma plantago-aquatica subsp. orientale |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| (R)-mevalonate + CoA + 2 NADP+ | Alisma plantago-aquatica subsp. orientale | - |
(S)-3-hydroxy-3-methylglutaryl-CoA + 2 NADPH + 2 H+ | - |
r |  |
| additional information | Alisma plantago-aquatica subsp. orientale | the enzyme is involved in the synthesis of the protostane triterpene Alisol B 23-acetate | ? | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Alisma plantago-aquatica subsp. orientale | F1DTB9 | - |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| recombinant solubilized enzyme from Escherichia coli strain BL21(DE3) Rosetta by nickel affinity chromatography and dialysis | Alisma plantago-aquatica subsp. orientale |
Renatured (Commentary)
| Renatured (Comment) | Organism |
|---|---|
| recombinant enzyme from inclusion bodies after expression in Escherichia coli strain BL21(DE3) Rosetta | Alisma plantago-aquatica subsp. orientale |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| (R)-mevalonate + CoA + 2 NADP+ | - |
Alisma plantago-aquatica subsp. orientale | (S)-3-hydroxy-3-methylglutaryl-CoA + 2 NADPH + 2 H+ | - |
r | |
| additional information | the enzyme is involved in the synthesis of the protostane triterpene Alisol B 23-acetate | Alisma plantago-aquatica subsp. orientale | ? | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| ? | x * 64600, about, sequence calculation, x * 66500, recombinant enzyme, SDS-PAGE | Alisma plantago-aquatica subsp. orientale |
| More | tertiary structure modelling, overview | Alisma plantago-aquatica subsp. orientale |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| 3-hydroxy-3-methylglutaryl-CoA reductase | - |
Alisma plantago-aquatica subsp. orientale |
| HMGR | - |
Alisma plantago-aquatica subsp. orientale |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 30 | - |
assay at, reverse reaction | Alisma plantago-aquatica subsp. orientale |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7.2 | - |
assay at, reverse reaction | Alisma plantago-aquatica subsp. orientale |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| NADP+ | - |
Alisma plantago-aquatica subsp. orientale | |
| NADPH | - |
Alisma plantago-aquatica subsp. orientale | |
pI Value
| Organism | Comment | pI Value Maximum | pI Value |
|---|---|---|---|
| Alisma plantago-aquatica subsp. orientale | sequence calculation | - |
7.87 |
General Information
| General Information | Comment | Organism |
|---|---|---|
| metabolism | the enzyme a key enzyme in the synthetic pathway of protostane triterpenes, including Alisol B 23-acetate and its derivatives | Alisma plantago-aquatica subsp. orientale |
Use of this online version of BRENDA is free under the CC BY 4.0 license. See terms of use for full details.
Release 2023.1 (January 2023)
Legal
Curated at
Member of
