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Literature summary for 1.1.1.343 extracted from

  • Chistoserdova, L.; Gomelsky, L.; Vorholt, J.A.; Gomelsky, M.; Tsygankov, Y.D.; Lidstrom, M.E.
    Analysis of two formaldehyde oxidation pathways in Methylobacillus flagellatus KT, a ribulose monophosphate cycle methylotroph (2000), Microbiology, 146, 233-238.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli DF214 cells Methylobacillus flagellatus

Organism

Organism UniProt Comment Textmining
Methylobacillus flagellatus
-
-
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [Āµmol/min/mg] Specific Activity Maximum [Āµmol/min/mg] Comment Organism
0.2
-
using NADP+ as cosubstrate, pH and temperature not specified in the publication Methylobacillus flagellatus
4
-
using NAD+ as cosubstrate, pH and temperature not specified in the publication Methylobacillus flagellatus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
6-phospho-D-gluconate + NAD+ highest activity Methylobacillus flagellatus D-ribulose 5-phosphate + CO2 + NADH + H+
-
?
6-phospho-D-gluconate + NADP+ lowest activity Methylobacillus flagellatus D-ribulose 5-phosphate + CO2 + NADPH + H+
-
?

Synonyms

Synonyms Comment Organism
6-phospho-gluconate dehydrogenase
-
Methylobacillus flagellatus
gndA
-
Methylobacillus flagellatus

Cofactor

Cofactor Comment Organism Structure
NAD+ best cofactor Methylobacillus flagellatus