Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 1.1.1.343 extracted from

  • Sosa-Saavedra, F.; Leˇn-Barrios, M.; Perez-Galdona, R.
    Pentose phosphate pathway as the main route for hexose catabolism in bradyrhizobium sp. lacking Entner-Doudoroff pathway. A role for NAD+-dependent 6-phosphogluconate dehydrogenase (decarboxylating) (2001), Soil Biol. Biochem., 33, 339-343.
    View publication on PubMed

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
33000
-
4 * 33000, SDS-PAGE Bradyrhizobium sp.
130000
-
gel filtration Bradyrhizobium sp.

Organism

Organism UniProt Comment Textmining
Bradyrhizobium japonicum
-
-
-
Bradyrhizobium japonicum USDA 110
-
-
-
Bradyrhizobium sp.
-
-
-
Bradyrhizobium sp. BGA-1
-
-
-

Purification (Commentary)

Purification (Comment) Organism
ammonium sulfate precipitation, Blue-Sepharose CL-6B column chromatography, DEAE-Sephacel gel filtration, and Sephadex G200 gel filtration Bradyrhizobium sp.

Specific Activity [micromol/min/mg]

Specific Activity Minimum [┬Ámol/min/mg] Specific Activity Maximum [┬Ámol/min/mg] Comment Organism
0.089
-
crude extract, at 37┬░C and pH 8.3 Bradyrhizobium sp.
31.16
-
after 350fold purification, at 37┬░C and pH 8.3 Bradyrhizobium sp.

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
6-phospho-D-gluconate + NAD+
-
Bradyrhizobium japonicum D-ribulose 5-phosphate + CO2 + NADH + H+
-
?
6-phospho-D-gluconate + NAD+
-
Bradyrhizobium sp. D-ribulose 5-phosphate + CO2 + NADH + H+
-
?
6-phospho-D-gluconate + NAD+
-
Bradyrhizobium japonicum USDA 110 D-ribulose 5-phosphate + CO2 + NADH + H+
-
?
6-phospho-D-gluconate + NAD+
-
Bradyrhizobium sp. BGA-1 D-ribulose 5-phosphate + CO2 + NADH + H+
-
?
additional information no NADP-dependent activity is detected Bradyrhizobium japonicum ?
-
?
additional information no NADP-dependent activity is detected Bradyrhizobium sp. ?
-
?
additional information no NADP-dependent activity is detected Bradyrhizobium japonicum USDA 110 ?
-
?
additional information no NADP-dependent activity is detected Bradyrhizobium sp. BGA-1 ?
-
?

Subunits

Subunits Comment Organism
homotetramer 4 * 33000, SDS-PAGE Bradyrhizobium sp.

Synonyms

Synonyms Comment Organism
NAD+-dependent 6-phosphogluconate dehydrogenase (decarboxylating)
-
Bradyrhizobium japonicum
NAD+-dependent 6-phosphogluconate dehydrogenase (decarboxylating)
-
Bradyrhizobium sp.
NAD-dependent PGDH
-
Bradyrhizobium japonicum
NAD-dependent PGDH
-
Bradyrhizobium sp.

Temperature Stability [┬░C]

Temperature Stability Minimum [┬░C] Temperature Stability Maximum [┬░C] Comment Organism
60
-
the enzyme is stable after a treatment at 60┬░C for 30 min Bradyrhizobium sp.

Cofactor

Cofactor Comment Organism Structure
NAD+
-
Bradyrhizobium japonicum
NAD+
-
Bradyrhizobium sp.

General Information

General Information Comment Organism
metabolism the enzyme commits hexoses to the pentose phosphate pathway Bradyrhizobium japonicum
metabolism the enzyme commits hexoses to the pentose phosphate pathway Bradyrhizobium sp.