Any feedback?
Literature summary for 1.1.1.345 extracted from
- Potential of R-2-hydroxyisocaproate dehydrogenase from Lactobacillus casei for stereospecific reductions (1992), Enzyme Microb. Technol., 14, 28-35.
No PubMed abstract available
Application
| Application | Comment | Organism |
|---|---|---|
| synthesis | the enzyme can be utilized for preparation of enantiomerically pure (R)-2-hydroxy-4-methylpentanoate in 88% yield, using formate dehydrogenase recycling of the NADH coenzyme | Lacticaseibacillus casei |
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| overexpression in Escherichia coli | Lacticaseibacillus casei |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.12 | - |
2-oxopentanoate | pH 7.0, temperature not specified in the publication | Lacticaseibacillus casei |  |
| 0.12 | - |
2-oxooctanoate | pH 7.0, temperature not specified in the publication | Lacticaseibacillus casei | |
| 0.13 | - |
2-Oxohexanoate | pH 7.0, temperature not specified in the publication | Lacticaseibacillus casei | |
| 0.2 | - |
phenylpyruvate | pH 7.0, temperature not specified in the publication | Lacticaseibacillus casei | |
| 0.4 | - |
4-methyl-2-oxopentanoate | pH 7.0, temperature not specified in the publication | Lacticaseibacillus casei | |
| 0.6 | - |
3-(4-hydroxyphenyl)-2-oxopropanoate | pH 7.0, temperature not specified in the publication | Lacticaseibacillus casei | |
| 1.6 | - |
2-Hydroxyoctanoate | pH 7.0, temperature not specified in the publication | Lacticaseibacillus casei | |
| 2.3 | - |
(R)-2-hydroxy-4-methylpentanoate | pH 7.0, temperature not specified in the publication | Lacticaseibacillus casei | |
| 2.7 | - |
2-hydroxyhexanoate | pH 7.0, temperature not specified in the publication | Lacticaseibacillus casei | |
| 3.3 | - |
2-hydroxypentanoate | pH 7.0, temperature not specified in the publication | Lacticaseibacillus casei | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Lacticaseibacillus casei | - |
- |
- |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| (R)-2-hydroxy-4-methylpentanoate + NAD+ | i.e. (R)-2-hydroxyisocaproate | Lacticaseibacillus casei | 4-methyl-2-oxopentanoate + NADH + H+ | i.e. 2-oxoisocaproate | r | |
| 2-hydroxyhexanoate + NAD+ | - |
Lacticaseibacillus casei | 2-oxohexanoate + NADH + H+ | - |
r | |
| 2-hydroxyoctanoate + NAD+ | - |
Lacticaseibacillus casei | 2-oxooctanoate + NADH + H+ | - |
r | |
| 2-hydroxypentanoate + NAD+ | - |
Lacticaseibacillus casei | 2-oxopentanoate + NADH + H+ | - |
r | |
| 2-oxohexanoate + NADH + H+ | i.e. 2-oxovalerate | Lacticaseibacillus casei | 2-hydroxyhexanoate + NAD+ | - |
r | |
| 2-oxooctanoate + NADH + H+ | - |
Lacticaseibacillus casei | 2-hydroxyoctanoate + NAD+ | - |
r | |
| 2-oxopentanoate + NADH + H+ | i.e. 2-oxocaproate | Lacticaseibacillus casei | 2-hydroxypentanoate + NAD+ | - |
r | |
| 3-(4-hydroxyphenyl)-2-oxopropanoate + NADH + H+ | - |
Lacticaseibacillus casei | 2-hydroxy-3-(4-hydroxyphenyl)propanoate + NAD+ | - |
? | |
| 4-methyl-2-oxopentanoate + NADH + H+ | i.e. 2-oxoisocaproate | Lacticaseibacillus casei | (R)-2-hydroxy-4-methylpentanoate + NAD+ | i.e. (R)-2-hydroxyisocaproate | r | |
| phenylpyruvate + NADH + H+ | - |
Lacticaseibacillus casei | phenyllactate + NAD+ | - |
? | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| R-HicDH | - |
Lacticaseibacillus casei |
Turnover Number [1/s]
| Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 15 | - |
2-Hydroxyoctanoate | pH 7.0, temperature not specified in the publication | Lacticaseibacillus casei | |
| 24 | - |
2-hydroxypentanoate | pH 7.0, temperature not specified in the publication | Lacticaseibacillus casei | |
| 26 | - |
2-hydroxyhexanoate | pH 7.0, temperature not specified in the publication | Lacticaseibacillus casei | |
| 43 | - |
(R)-2-hydroxy-4-methylpentanoate | pH 7.0, temperature not specified in the publication | Lacticaseibacillus casei | |
| 84 | - |
4-methyl-2-oxopentanoate | pH 7.0, temperature not specified in the publication | Lacticaseibacillus casei | |
| 139 | - |
2-Oxohexanoate | pH 7.0, temperature not specified in the publication | Lacticaseibacillus casei | |
| 197 | - |
2-oxopentanoate | pH 7.0, temperature not specified in the publication | Lacticaseibacillus casei | |
| 788 | - |
3-(4-hydroxyphenyl)-2-oxopropanoate | pH 7.0, temperature not specified in the publication | Lacticaseibacillus casei | |
| 968 | - |
phenylpyruvate | pH 7.0, temperature not specified in the publication | Lacticaseibacillus casei | |
| 1200 | - |
2-oxooctanoate | pH 7.0, temperature not specified in the publication | Lacticaseibacillus casei | |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7 | - |
assay at | Lacticaseibacillus casei |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| NADH | - |
Lacticaseibacillus casei | |
kcat/KM [mM/s]
| kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 7.2 | - |
2-hydroxypentanoate | pH 7.0, temperature not specified in the publication | Lacticaseibacillus casei | |
| 9.7 | - |
2-hydroxyhexanoate | pH 7.0, temperature not specified in the publication | Lacticaseibacillus casei | |
| 18 | - |
(R)-2-hydroxy-4-methylpentanoate | pH 7.0, temperature not specified in the publication | Lacticaseibacillus casei | |
| 25 | - |
2-Hydroxyoctanoate | pH 7.0, temperature not specified in the publication | Lacticaseibacillus casei | |
| 139 | - |
2-oxooctanoate | pH 7.0, temperature not specified in the publication | Lacticaseibacillus casei | |
| 210 | - |
4-methyl-2-oxopentanoate | pH 7.0, temperature not specified in the publication | Lacticaseibacillus casei | |
| 1100 | - |
2-Oxohexanoate | pH 7.0, temperature not specified in the publication | Lacticaseibacillus casei | |
| 1300 | - |
3-(4-hydroxyphenyl)-2-oxopropanoate | pH 7.0, temperature not specified in the publication | Lacticaseibacillus casei | |
| 1600 | - |
2-oxopentanoate | pH 7.0, temperature not specified in the publication | Lacticaseibacillus casei | |
| 4800 | - |
phenylpyruvate | pH 7.0, temperature not specified in the publication | Lacticaseibacillus casei | |
Use of this online version of BRENDA is free under the CC BY 4.0 license. See terms of use for full details.
Release 2023.1 (January 2023)
Legal
Curated at
Member of
