KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.01 | - |
NADPH | at pH 6.4 and 30°C | Brevibacterium ketosoreductum | |
5 | - |
2,5-didehydro-D-gluconate | at pH 6.4 and 30°C | Brevibacterium ketosoreductum |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
35000 | - |
2 * 35000, SDS-PAGE | Brevibacterium ketosoreductum |
72000 | - |
gel filtration | Brevibacterium ketosoreductum |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Brevibacterium ketosoreductum | - |
- |
- |
Brevibacterium ketosoreductum ATCC 21914 | - |
- |
- |
Purification (Comment) | Organism |
---|---|
ammonium sulfate precipitation, DEAE Toyopearl column chromatography and phenyl Toyopearl column chromatography, Blue Sepharose column chromatography and Sephacryl S-200 gel filtration | Brevibacterium ketosoreductum |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
0.07 | - |
crude extract, at pH 6.4 and 30°C | Brevibacterium ketosoreductum |
22.2 | - |
after 317fold purification, at pH 6.4 and 30°C | Brevibacterium ketosoreductum |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
2,5-didehydro-D-gluconate + NADPH + H+ | 100% activity | Brevibacterium ketosoreductum | 5-dehydro-D-gluconate + NADP+ | - |
? | |
2-dehydro-D-gluconate + NADPH + H+ | 11.8% activity compared to 2,5-didehydro-D-gluconate | Brevibacterium ketosoreductum | D-gluconate + NADP+ | - |
? | |
2-dehydro-L-gulonate + NADPH + H+ | 10.8% activity compared to 2,5-didehydro-D-gluconate | Brevibacterium ketosoreductum | L-idonate + NADP+ | - |
? | |
additional information | no activity with D-gluconate, D-fructose, L-sorbose, and 5-dehydro-D-gluconate | Brevibacterium ketosoreductum | ? | - |
? | |
additional information | no activity with D-gluconate, D-fructose, L-sorbose, and 5-dehydro-D-gluconate | Brevibacterium ketosoreductum ATCC 21914 | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
homodimer | 2 * 35000, SDS-PAGE | Brevibacterium ketosoreductum |
Synonyms | Comment | Organism |
---|---|---|
25DKG reductase | - |
Brevibacterium ketosoreductum |
2KR | - |
Brevibacterium ketosoreductum |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
6 | - |
- |
Brevibacterium ketosoreductum |
pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|
5.2 | 10 | after 2 h incubation at 30°C, the remaining activity is about 35% of the original level at pH 8.4, and no remaining activity is observed below pH 5.1 and 10.0. The enzyme is stable from pH 6.0 to 7.5 | Brevibacterium ketosoreductum |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NADPH | - |
Brevibacterium ketosoreductum |
Organism | Comment | pI Value Maximum | pI Value |
---|---|---|---|
Brevibacterium ketosoreductum | isoelectric focusing | - |
4.7 |