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Literature summary for 1.1.1.35 extracted from

  • Shimakata, T.; Fujita, Y.; Kusaka, T.
    Purification and characterization of 3-hydroxyacyl-CoA dehydrogenase of Mycobacterium smegmatis (1979), J. Biochem., 86, 1191-1198.
    View publication on PubMed

Inhibitors

Inhibitors Comment Organism Structure
p-chloromercuriphenyl sulfonic acid 95% inhibition at 0.1 mM Mycolicibacterium smegmatis

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.033
-
NADH
-
Mycolicibacterium smegmatis
0.036
-
acetoacetyl-CoA
-
Mycolicibacterium smegmatis
0.083
-
NAD+
-
Mycolicibacterium smegmatis
1.19
-
acetoacetyl-pantetheine
-
Mycolicibacterium smegmatis
44.4
-
acetoacetyl-N-acetyl-cysteamine
-
Mycolicibacterium smegmatis

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
50300
-
gel filtration Mycolicibacterium smegmatis

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
(S)-3-hydroxybutyryl-CoA + NAD+ Mycolicibacterium smegmatis
-
acetoacetyl-CoA + NADH
-
r

Organism

Organism UniProt Comment Textmining
Mycolicibacterium smegmatis
-
-
-

Purification (Commentary)

Purification (Comment) Organism
ammonium sulfate, Sephadex G-150, hydroxyapatite, NAD-Sepharose 4B Mycolicibacterium smegmatis

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
41.2
-
-
Mycolicibacterium smegmatis

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
(S)-3-hydroxybutyryl-CoA + NAD+
-
Mycolicibacterium smegmatis acetoacetyl-CoA + NADH
-
r
(S)-3-hydroxybutyryl-CoA + NADP+
-
Mycolicibacterium smegmatis acetoacetyl-CoA + NADPH
-
?
acetoacetyl-N-acetylcysteamine + NADH
-
Mycolicibacterium smegmatis (S)-3-hydroxybutyryl-N-acetylcysteamine + NAD+
-
?
acetoacetyl-pantetheine + NADH
-
Mycolicibacterium smegmatis (S)-3-hydroxybutyryl-pantetheine
-
?

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
6
-
reduction of acetoacetyl-CoA Mycolicibacterium smegmatis
9
-
oxidation of (S)-3-hydroxybutyryl-CoA Mycolicibacterium smegmatis

Cofactor

Cofactor Comment Organism Structure
NAD+
-
Mycolicibacterium smegmatis
NADP+
-
Mycolicibacterium smegmatis