KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.05 | - |
acetoacetyl-CoA | pH 6.5 | Euglena gracilis |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
mitochondrion | - |
Euglena gracilis | 5739 | - |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
32000 | - |
1 * 45500 + 1 * 44500 + 1 * 34000 + 1 * 32000, SDS-PAGE | Euglena gracilis |
34000 | - |
1 * 45500 + 1 * 44500 + 1 * 34000 + 1 * 32000, SDS-PAGE | Euglena gracilis |
44500 | - |
1 * 45500 + 1 * 44500 + 1 * 34000 + 1 * 32000, SDS-PAGE | Euglena gracilis |
45500 | - |
1 * 45500 + 1 * 44500 + 1 * 34000 + 1 * 32000, SDS-PAGE | Euglena gracilis |
460000 | - |
native enzyme complex, sucrose density gradient centrifugation | Euglena gracilis |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
(S)-3-hydroxybutyryl-CoA + NAD+ | Euglena gracilis | both enzyme activities are specific for L-isomers | acetoacetyl-CoA + NADH | - |
r | |
(S)-3-hydroxybutyryl-CoA + NAD+ | Euglena gracilis 1224-5/25 | both enzyme activities are specific for L-isomers | acetoacetyl-CoA + NADH | - |
r |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Euglena gracilis | - |
multifunctional beta-oxidation enzyme complex composed of 2 different 3-hydroyacyl-CoA dehydrogenases, 2 different 2-enoyl-CoA hydratases, thiolase, and epimerase activities | - |
Euglena gracilis 1224-5/25 | - |
multifunctional beta-oxidation enzyme complex composed of 2 different 3-hydroyacyl-CoA dehydrogenases, 2 different 2-enoyl-CoA hydratases, thiolase, and epimerase activities | - |
Purification (Comment) | Organism |
---|---|
beta-oxidation enzyme complex 67fold to homogeneity by ammonium sulfate precipitation, density gradient centrifugation, and ion-exchange chromatography | Euglena gracilis |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
40 | - |
purified enzyme | Euglena gracilis |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
(S)-3-hydroxybutyryl-CoA + NAD+ | both enzyme activities are specific for L-isomers | Euglena gracilis | acetoacetyl-CoA + NADH | - |
r | |
(S)-3-hydroxybutyryl-CoA + NAD+ | both enzyme activities are specific for L-isomers | Euglena gracilis 1224-5/25 | acetoacetyl-CoA + NADH | - |
r | |
acetoacetyl-CoA + NADH + H+ | - |
Euglena gracilis | (S)-3-hydroxybutyryl-CoA + NAD+ | both enzyme activities are specific for L-isomers | r | |
acetoacetyl-CoA + NADH + H+ | - |
Euglena gracilis 1224-5/25 | (S)-3-hydroxybutyryl-CoA + NAD+ | both enzyme activities are specific for L-isomers | r |
Subunits | Comment | Organism |
---|---|---|
More | the hydratase and the thiolase functions are located on the large subunit, whereas 2 dehydrogenase functions are located on the 2 smaller subunits, epimerase activity is only measurable in the complete enzyme complex | Euglena gracilis |
tetramer | 1 * 45500 + 1 * 44500 + 1 * 34000 + 1 * 32000, SDS-PAGE | Euglena gracilis |
Synonyms | Comment | Organism |
---|---|---|
3-hydroxyacyl-coenzyme A dehydrogenase | - |
Euglena gracilis |
multifunctional beta-oxidation enzyme | - |
Euglena gracilis |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
6.5 | - |
- |
Euglena gracilis |