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Literature summary for 1.1.1.35 extracted from

  • Ramos-Vera, W.H.; Weiss, M.; Strittmatter, E.; Kockelkorn, D.; Fuchs, G.
    Identification of missing genes and enzymes for autotrophic carbon fixation in crenarchaeota (2011), J. Bacteriol., 193, 1201-1211.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

Cloned (Comment) Organism
expression in Escherichia coli Metallosphaera sedula

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.2
-
(S)-3-hydroxybutanoyl-CoA pH 8.0, 65°C Metallosphaera sedula
0.25
-
NAD+ pH 8.0, 65°C Metallosphaera sedula

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
70000
-
x * 70000, SDS-PAGE Metallosphaera sedula

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
(S)-3-hydroxybutanoyl-CoA + NAD+ Metallosphaera sedula the enzyme is involved in autotrophic carbon fixation acetoacetyl-CoA + NADH + H+
-
?
(S)-3-hydroxybutanoyl-CoA + NAD+ Metallosphaera sedula DSM 5348 the enzyme is involved in autotrophic carbon fixation acetoacetyl-CoA + NADH + H+
-
?

Organism

Organism UniProt Comment Textmining
Metallosphaera sedula A4YDS4
-
-
Metallosphaera sedula DSM 5348 A4YDS4
-
-

Purification (Commentary)

Purification (Comment) Organism
-
Metallosphaera sedula

Source Tissue

Source Tissue Comment Organism Textmining
culture condition:autotrophically-grown cell bifunctional crotonyl-CoA hydratase/(S)-3-hydroxybutanoyl-CoA dehydrogenase Metallosphaera sedula
-
culture condition:heterotrophically-grown cell bifunctional crotonyl-CoA hydratase/(S)-3-hydroxybutanoyl-CoA dehydrogenase Metallosphaera sedula
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3 8 pH 8.0, 65°C, native enzyme, autotrophically- or heterotrophically-grown cell Metallosphaera sedula

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
(S)-3-hydroxybutanoyl-CoA + NAD+ the enzyme is involved in autotrophic carbon fixation Metallosphaera sedula acetoacetyl-CoA + NADH + H+
-
?
(S)-3-hydroxybutanoyl-CoA + NAD+ no activity with (R)-3-hydroxybutanoyl-CoA, no activity with NADP+. Bifunctional crotonyl-CoA hydratase/(S)-3-hydroxybutanoyl-CoA dehydrogenase (EC 4.2.1.150/EC 1.1.1.35) Metallosphaera sedula acetoacetyl-CoA + NADH + H+
-
?
(S)-3-hydroxybutanoyl-CoA + NAD+ the enzyme is involved in autotrophic carbon fixation Metallosphaera sedula DSM 5348 acetoacetyl-CoA + NADH + H+
-
?
(S)-3-hydroxybutanoyl-CoA + NAD+ no activity with (R)-3-hydroxybutanoyl-CoA, no activity with NADP+. Bifunctional crotonyl-CoA hydratase/(S)-3-hydroxybutanoyl-CoA dehydrogenase (EC 4.2.1.150/EC 1.1.1.35) Metallosphaera sedula DSM 5348 acetoacetyl-CoA + NADH + H+
-
?

Subunits

Subunits Comment Organism
? x * 70000, SDS-PAGE Metallosphaera sedula

Synonyms

Synonyms Comment Organism
Msed_0399 locus name, bifunctional crotonyl-CoA hydratase/(S)-3-hydroxybutanoyl-CoA dehydrogenase (EC 4.2.1.150/EC 1.1.1.35) Metallosphaera sedula

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
65
-
assay at Metallosphaera sedula

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8
-
assay at Metallosphaera sedula

Cofactor

Cofactor Comment Organism Structure
NAD+ no activity with NADP+ Metallosphaera sedula

General Information

General Information Comment Organism
physiological function the enzyme is involved in autotrophic carbon fixation Metallosphaera sedula