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Literature summary for extracted from

  • Chen, J.; Gao, X.; Hong, L.; Ma, L.; Li, Y.
    Expression, purification and functional characterization of a novel 3alpha-hydroxysteroid dehydrogenase from Pseudomonas aeruginosa (2015), Protein Expr. Purif., 115, 102-108 .
    View publication on PubMed


Cloned (Comment) Organism
sequence comparison and phylogenetic analysis, recombinant expression of His-tagged enzyme in Escherichia coli strain BL21(DE3) Pseudomonas aeruginosa


Inhibitors Comment Organism Structure
Pseudomonas aeruginosa
Pseudomonas aeruginosa
Cu2+ 67% inhibition at 0.5 mM Pseudomonas aeruginosa

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
additional information
additional information steady-state kinetics Pseudomonas aeruginosa


Metals/Ions Comment Organism Structure
Fe3+ activates Pseudomonas aeruginosa
K+ activates Pseudomonas aeruginosa
Mn2+ activates Pseudomonas aeruginosa
additional information no effect by 0.5 mM of Mg2+, Ni2+, and Al3+ Pseudomonas aeruginosa
Na+ activates Pseudomonas aeruginosa
Zn2+ activates 1.75fold at 0.5 mM Pseudomonas aeruginosa

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
a 3alpha-hydroxysteroid + NAD+ Pseudomonas aeruginosa
a 3-oxosteroid + NADH + H+


Organism UniProt Comment Textmining
Pseudomonas aeruginosa Q9I1X3

Purification (Commentary)

Purification (Comment) Organism
recombinant His-tagged enzyme from Escherichia coli strain BL21(DE3) by nickel affinity chromatography Pseudomonas aeruginosa

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
a 3alpha-hydroxysteroid + NAD+
Pseudomonas aeruginosa a 3-oxosteroid + NADH + H+
cholate + NAD+
Pseudomonas aeruginosa 7alpha,12alpha-dihydroxy-3-oxo-5beta-cholan-24-oic acid + NADH + H+


Subunits Comment Organism
? x * 32000, recombinant His-tagged enzyme, SDS-PAGE Pseudomonas aeruginosa


Synonyms Comment Organism
Pseudomonas aeruginosa
3alpha-hydroxysteroid dehydrogenase
Pseudomonas aeruginosa

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
recombinant enzyme Pseudomonas aeruginosa

Temperature Range [°C]

Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
15 45 optimum at 30°C, over 60% of maximal activity in the range of 25-45°C, with the temperature increasing from 30°C to 45°C, the enzyme activity gradually decreases to 77.55% of the optimum whereas with the temperature decreasing from 30°C to 15°C, the enzyme activity rapidly drops to 39.4% of the optimum Pseudomonas aeruginosa

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
purified recombinant His-tagged enzyme, stable up to Pseudomonas aeruginosa

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
recombinant enzyme Pseudomonas aeruginosa

pH Range

pH Minimum pH Maximum Comment Organism
4 11 activity range, profile overview Pseudomonas aeruginosa


Cofactor Comment Organism Structure
Pseudomonas aeruginosa
Pseudomonas aeruginosa

General Information

General Information Comment Organism
physiological function 3alpha-hydroxysteroid dehydrogenase catalyzes the oxidation of the 3-hydroxyl group of steroids. The enzymatic conversion is a critical step in the enzymatic assay of urinary sulfated bile acids Pseudomonas aeruginosa