Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli Rosetta(DE3) cells | Candida parapsilosis |
Crystallization (Comment) | Organism |
---|---|
sitting drop vapor diffusion method, using 0.1 M Tris-HCl (pH 8.5), 25% (w/v) PEG3350, and 0.2 M NaCl | Candida parapsilosis |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
2-dehydropantolactone + NADPH + H+ | Candida parapsilosis | - |
(R)-pantolactone + NADP+ | - |
? | |
2-dehydropantolactone + NADPH + H+ | Candida parapsilosis IFO 0708 | - |
(R)-pantolactone + NADP+ | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Candida parapsilosis | Q76L36 | - |
- |
Candida parapsilosis IFO 0708 | Q76L36 | - |
- |
Purification (Comment) | Organism |
---|---|
Ni Sepharose column chromatography, Resource Q column chromatography, and Superdex 75 gel filtration | Candida parapsilosis |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
2-dehydropantolactone + NADPH + H+ | - |
Candida parapsilosis | (R)-pantolactone + NADP+ | - |
? | |
2-dehydropantolactone + NADPH + H+ | - |
Candida parapsilosis IFO 0708 | (R)-pantolactone + NADP+ | - |
? |
Synonyms | Comment | Organism |
---|---|---|
conjugated polyketone reductase | - |
Candida parapsilosis |
CPR-C1 | - |
Candida parapsilosis |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NADPH | dependent on | Candida parapsilosis |