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Literature summary for 1.1.1.359 extracted from

  • Lamble, H.J.; Heyer, N.I.; Bull, S.D.; Hough, D.W.; Danson, M.J.
    Metabolic pathway promiscuity in the archaeon Sulfolobus solfataricus revealed by studies on glucose dehydrogenase and 2-keto-3-deoxygluconate aldolase (2003), J. Biol. Chem., 278, 34066-34072.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
expression in Escherichia coli Saccharolobus solfataricus

Inhibitors

Inhibitors Comment Organism Structure
EDTA 10 mM, 60% loss of activity Saccharolobus solfataricus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.44
-
D-galactose pH 7.5, 70°C, cofactor: NADP+ Saccharolobus solfataricus
0.57
-
D-galactose pH 7.5, 70°C, cofactor: NAD+ Saccharolobus solfataricus
1.3
-
beta-D-glucose pH 7.5, 70°C, cofactor: NADP+ Saccharolobus solfataricus
1.5
-
beta-D-glucose pH 7.5, 70°C, cofactor: NAD+ Saccharolobus solfataricus

Metals/Ions

Metals/Ions Comment Organism Structure
additional information no significant increase in the presence of ZnCl2, CaCl2, or MgCl2, at final concentrations of 0.1 mM Saccharolobus solfataricus
Zinc the enzyme possess the catalytic zinc binding residues Cys-39 and His-66 Saccharolobus solfataricus

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
40849
-
4 * 40849, calculated from sequence Saccharolobus solfataricus
41000
-
4 * 41000, SDS-PAGE Saccharolobus solfataricus
160000
-
gel filtration Saccharolobus solfataricus

Organism

Organism UniProt Comment Textmining
Saccharolobus solfataricus O93715
-
-

Purification (Commentary)

Purification (Comment) Organism
enzyme from Sulfolobus solfataricus cell extract and recombinant enzyme expressed in Escherichia coli Saccharolobus solfataricus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
6-deoxy-D-glucose + NAD+ + H2O
-
Saccharolobus solfataricus ?
-
?
6-deoxy-D-glucose + NADP+ + H2O
-
Saccharolobus solfataricus ?
-
?
beta-D-glucose + NAD+ + H2O kcat/Km for beta-D-glucose in the reaction with NAD+ is 1.4fold higher than the kcat/Km value for beta-D-glucose in the reaction with NADP+ Saccharolobus solfataricus D-gluconate + NADH + 2 H+
-
ir
beta-D-glucose + NADP+ + H2O kcat/Km for beta-D-glucose in the reaction with NADP+ is 1.4fold lower than the kcat/Km value for beta-D-glucose in the reaction with NAD+ Saccharolobus solfataricus D-gluconate + NADPH + 2 H+
-
ir
D-fucose + NAD+ + H2O
-
Saccharolobus solfataricus ?
-
?
D-fucose + NADP+ + H2O
-
Saccharolobus solfataricus ?
-
?
D-galactose + NAD+ + H2O
-
Saccharolobus solfataricus D-galactonate + NADH + 2 H+
-
?
D-galactose + NADP+ + H2O
-
Saccharolobus solfataricus D-galactonate + NADPH + 2 H+
-
?
D-xylose + NAD+ + H2O
-
Saccharolobus solfataricus ?
-
?
D-xylose + NADP+ + H2O
-
Saccharolobus solfataricus ?
-
?
L-arabinose + NAD+ + H2O
-
Saccharolobus solfataricus ?
-
?
L-arabinose + NADP+ + H2O
-
Saccharolobus solfataricus ?
-
?

Subunits

Subunits Comment Organism
tetramer 4 * 41000, SDS-PAGE Saccharolobus solfataricus
tetramer 4 * 40849, calculated from sequence Saccharolobus solfataricus

Synonyms

Synonyms Comment Organism
glucose dehydrogenase
-
Saccharolobus solfataricus

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
70
-
assay at Saccharolobus solfataricus

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
37.4
-
D-galactose pH 7.5, 70°C, cofactor: NADP+ Saccharolobus solfataricus
47.7
-
beta-D-glucose pH 7.5, 70°C, cofactor: NADP+ Saccharolobus solfataricus
61.3
-
D-galactose pH 7.5, 70°C, cofactor: NAD+ Saccharolobus solfataricus
74.9
-
beta-D-glucose pH 7.5, 70°C, cofactor: NAD+ Saccharolobus solfataricus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.5
-
assay at Saccharolobus solfataricus

Cofactor

Cofactor Comment Organism Structure
NAD+ kcat/Km for beta-D-glucose in the reaction with NAD+ is 1.4fold higher than the kcat/Km value for beta-D-glucose in the reaction with NADP+ Saccharolobus solfataricus
NADP+ kcat/Km for beta-D-glucose in the reaction with NADP+ is 1.4fold lower than the kcat/Km value for beta-D-glucose in the reaction with NAD+ Saccharolobus solfataricus

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
36.7
-
beta-D-glucose pH 7.5, 70°C, cofactor: NADP+ Saccharolobus solfataricus
49.9
-
beta-D-glucose pH 7.5, 70°C, cofactor: NAD+ Saccharolobus solfataricus
85.1
-
D-galactose pH 7.5, 70°C, cofactor: NADP+ Saccharolobus solfataricus
107
-
D-galactose pH 7.5, 70°C, cofactor: NAD+ Saccharolobus solfataricus