Cloned (Comment) | Organism |
---|---|
expression in Escherichia coli | Saccharolobus solfataricus |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
EDTA | 10 mM, 60% loss of activity | Saccharolobus solfataricus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.44 | - |
D-galactose | pH 7.5, 70°C, cofactor: NADP+ | Saccharolobus solfataricus | |
0.57 | - |
D-galactose | pH 7.5, 70°C, cofactor: NAD+ | Saccharolobus solfataricus | |
1.3 | - |
beta-D-glucose | pH 7.5, 70°C, cofactor: NADP+ | Saccharolobus solfataricus | |
1.5 | - |
beta-D-glucose | pH 7.5, 70°C, cofactor: NAD+ | Saccharolobus solfataricus |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
additional information | no significant increase in the presence of ZnCl2, CaCl2, or MgCl2, at final concentrations of 0.1 mM | Saccharolobus solfataricus | |
Zinc | the enzyme possess the catalytic zinc binding residues Cys-39 and His-66 | Saccharolobus solfataricus |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
40849 | - |
4 * 40849, calculated from sequence | Saccharolobus solfataricus |
41000 | - |
4 * 41000, SDS-PAGE | Saccharolobus solfataricus |
160000 | - |
gel filtration | Saccharolobus solfataricus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Saccharolobus solfataricus | O93715 | - |
- |
Purification (Comment) | Organism |
---|---|
enzyme from Sulfolobus solfataricus cell extract and recombinant enzyme expressed in Escherichia coli | Saccharolobus solfataricus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
6-deoxy-D-glucose + NAD+ + H2O | - |
Saccharolobus solfataricus | ? | - |
? | |
6-deoxy-D-glucose + NADP+ + H2O | - |
Saccharolobus solfataricus | ? | - |
? | |
beta-D-glucose + NAD+ + H2O | kcat/Km for beta-D-glucose in the reaction with NAD+ is 1.4fold higher than the kcat/Km value for beta-D-glucose in the reaction with NADP+ | Saccharolobus solfataricus | D-gluconate + NADH + 2 H+ | - |
ir | |
beta-D-glucose + NADP+ + H2O | kcat/Km for beta-D-glucose in the reaction with NADP+ is 1.4fold lower than the kcat/Km value for beta-D-glucose in the reaction with NAD+ | Saccharolobus solfataricus | D-gluconate + NADPH + 2 H+ | - |
ir | |
D-fucose + NAD+ + H2O | - |
Saccharolobus solfataricus | ? | - |
? | |
D-fucose + NADP+ + H2O | - |
Saccharolobus solfataricus | ? | - |
? | |
D-galactose + NAD+ + H2O | - |
Saccharolobus solfataricus | D-galactonate + NADH + 2 H+ | - |
? | |
D-galactose + NADP+ + H2O | - |
Saccharolobus solfataricus | D-galactonate + NADPH + 2 H+ | - |
? | |
D-xylose + NAD+ + H2O | - |
Saccharolobus solfataricus | ? | - |
? | |
D-xylose + NADP+ + H2O | - |
Saccharolobus solfataricus | ? | - |
? | |
L-arabinose + NAD+ + H2O | - |
Saccharolobus solfataricus | ? | - |
? | |
L-arabinose + NADP+ + H2O | - |
Saccharolobus solfataricus | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
tetramer | 4 * 41000, SDS-PAGE | Saccharolobus solfataricus |
tetramer | 4 * 40849, calculated from sequence | Saccharolobus solfataricus |
Synonyms | Comment | Organism |
---|---|---|
glucose dehydrogenase | - |
Saccharolobus solfataricus |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
70 | - |
assay at | Saccharolobus solfataricus |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
37.4 | - |
D-galactose | pH 7.5, 70°C, cofactor: NADP+ | Saccharolobus solfataricus | |
47.7 | - |
beta-D-glucose | pH 7.5, 70°C, cofactor: NADP+ | Saccharolobus solfataricus | |
61.3 | - |
D-galactose | pH 7.5, 70°C, cofactor: NAD+ | Saccharolobus solfataricus | |
74.9 | - |
beta-D-glucose | pH 7.5, 70°C, cofactor: NAD+ | Saccharolobus solfataricus |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.5 | - |
assay at | Saccharolobus solfataricus |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NAD+ | kcat/Km for beta-D-glucose in the reaction with NAD+ is 1.4fold higher than the kcat/Km value for beta-D-glucose in the reaction with NADP+ | Saccharolobus solfataricus | |
NADP+ | kcat/Km for beta-D-glucose in the reaction with NADP+ is 1.4fold lower than the kcat/Km value for beta-D-glucose in the reaction with NAD+ | Saccharolobus solfataricus |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
36.7 | - |
beta-D-glucose | pH 7.5, 70°C, cofactor: NADP+ | Saccharolobus solfataricus | |
49.9 | - |
beta-D-glucose | pH 7.5, 70°C, cofactor: NAD+ | Saccharolobus solfataricus | |
85.1 | - |
D-galactose | pH 7.5, 70°C, cofactor: NADP+ | Saccharolobus solfataricus | |
107 | - |
D-galactose | pH 7.5, 70°C, cofactor: NAD+ | Saccharolobus solfataricus |