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Literature summary for 1.1.1.363 extracted from

  • Acero-Navarro, K.E.; Jimenez-Ramirez, M.; Villalobos, M.A.; Vargas-Martinez, R.; Perales-Vela, H.V.; Velasco-Garcia, R.
    Cloning, overexpression, and purification of glucose-6-phosphate dehydrogenase of Pseudomonas aeruginosa (2018), Protein Expr. Purif., 142, 53-61 .
    View publication on PubMed
Search for more literature for 1.1.1.363

Cloned(Commentary)

Cloned (Comment) Organism
overexpression as a fusion protein in Escherichia coli Pseudomonas aeruginosa

Inhibitors

Inhibitors Comment Organism Structure
ATP
-
 Pseudomonas aeruginosa

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
250000
-
 non-denaturing PAGE reveals two bands: a major band of 250000 and a minor band of 500000 Pseudomonas aeruginosa
500000
-
 non-denaturing PAGE reveals two bands: a major band of 250000 and a minor band of 500000 Pseudomonas aeruginosa

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
6-phospho-D-gluconate + NAD+ Pseudomonas aeruginosa
-
 D-ribulose 5-phosphate + CO2 + NADH + H+
-
 ?
6-phospho-D-gluconate + NAD+ Pseudomonas aeruginosa ATCC 15692
-
 D-ribulose 5-phosphate + CO2 + NADH + H+
-
 ?
6-phospho-D-gluconate + NADP+ Pseudomonas aeruginosa the enzyme generates NADPH, an essential cofactor for several biosynthetic pathways and antioxidant enzymes D-ribulose 5-phosphate + CO2 + NADPH + H+
-
 ?
6-phospho-D-gluconate + NADP+ Pseudomonas aeruginosa ATCC 15692 the enzyme generates NADPH, an essential cofactor for several biosynthetic pathways and antioxidant enzymes D-ribulose 5-phosphate + CO2 + NADPH + H+
-
 ?

Organism

Organism UniProt Comment Textmining
Pseudomonas aeruginosa O68282
-
-
Pseudomonas aeruginosa ATCC 15692 O68282
-
-

Purification (Commentary)

Purification (Comment) Organism
a fast, efficient and reproducible procedure for the purification of the Pseudomonas aeruginosa enzyme that can be implemented in a short period (2 days). The method is based on protein precipitation with CaCl2 and further purification by ion exchange chromatography Pseudomonas aeruginosa

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
145.7
-
 pH 8.0, 30°C, NADP+ Pseudomonas aeruginosa

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
6-phospho-D-gluconate + NAD+
-
 Pseudomonas aeruginosa D-ribulose 5-phosphate + CO2 + NADH + H+
-
 ?
6-phospho-D-gluconate + NAD+
-
 Pseudomonas aeruginosa ATCC 15692 D-ribulose 5-phosphate + CO2 + NADH + H+
-
 ?
6-phospho-D-gluconate + NADP+
-
 Pseudomonas aeruginosa D-ribulose 5-phosphate + CO2 + NADPH + H+
-
 ?
6-phospho-D-gluconate + NADP+ the enzyme generates NADPH, an essential cofactor for several biosynthetic pathways and antioxidant enzymes Pseudomonas aeruginosa D-ribulose 5-phosphate + CO2 + NADPH + H+
-
 ?
6-phospho-D-gluconate + NADP+
-
 Pseudomonas aeruginosa ATCC 15692 D-ribulose 5-phosphate + CO2 + NADPH + H+
-
 ?
6-phospho-D-gluconate + NADP+ the enzyme generates NADPH, an essential cofactor for several biosynthetic pathways and antioxidant enzymes Pseudomonas aeruginosa ATCC 15692 D-ribulose 5-phosphate + CO2 + NADPH + H+
-
 ?

Synonyms

Synonyms Comment Organism
G6PDH
-
 Pseudomonas aeruginosa
glucose-6-phosphate dehydrogenase
-
 Pseudomonas aeruginosa

Ki Value [mM]

Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
0.3
-
 ATP pH 8.0, 30°C Pseudomonas aeruginosa

IC50 Value

IC50 Value IC50 Value Maximum Comment Organism Inhibitor Structure
0.162
-
 pH 8.0, 30°C Pseudomonas aeruginosa ATP

General Information

General Information Comment Organism
physiological function the enzyme generates NADPH, an essential cofactor for several biosynthetic pathways and antioxidant enzymes. It is a key enzyme in the metabolism of various carbon sources, such as glucose, glycerol, fructose, and mannitol Pseudomonas aeruginosa