Activating Compound | Comment | Organism | Structure |
---|---|---|---|
dithiothreitol | - |
Heliocidaris crassispina | |
Sodium phosphate | - |
Heliocidaris crassispina |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
ADP | - |
Heliocidaris crassispina | |
ATP | - |
Heliocidaris crassispina | |
Na2SO3 | - |
Heliocidaris crassispina | |
oxaloacetate | substrate inhibition | Heliocidaris crassispina | |
p-chloromercuriphenylsulfonate | - |
Heliocidaris crassispina | |
sulfite | - |
Heliocidaris crassispina | |
Zn2+ | - |
Heliocidaris crassispina |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.03 | - |
NADH | - |
Heliocidaris crassispina | |
0.6 | - |
NAD+ | - |
Heliocidaris crassispina | |
7 | - |
L-malate | - |
Heliocidaris crassispina |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
cytosol | - |
Heliocidaris crassispina | 5829 | - |
mitochondrion | - |
Heliocidaris crassispina | 5739 | - |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
CaCl2 | - |
Heliocidaris crassispina | |
MgCl2 | - |
Heliocidaris crassispina | |
Na2HAsO4 | - |
Heliocidaris crassispina | |
NaF | - |
Heliocidaris crassispina |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
35000 | - |
2 * 35000, SDS-PAGE | Heliocidaris crassispina |
65000 | - |
- |
Heliocidaris crassispina |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
malate + NAD+ | Heliocidaris crassispina | - |
oxaloacetate + NADH + H+ | - |
r | |
malate + NAD+ | Strongylocentrotus purpuratus | - |
oxaloacetate + NADH + H+ | - |
r |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Heliocidaris crassispina | - |
sea urchin | - |
Strongylocentrotus purpuratus | - |
sea urchin | - |
Purification (Comment) | Organism |
---|---|
- |
Strongylocentrotus purpuratus |
M-MDH | Heliocidaris crassispina |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
egg | - |
Heliocidaris crassispina | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
1.28 | - |
- |
Heliocidaris crassispina |
Storage Stability | Organism |
---|---|
-20°C, can be stored at least several months without any appreciable loss in activity | Heliocidaris crassispina |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
(S)-malate + NAD+ | - |
Heliocidaris crassispina | oxaloacetate + NADH + H+ | - |
r | |
(S)-malate + NAD+ | - |
Strongylocentrotus purpuratus | oxaloacetate + NADH + H+ | - |
r | |
malate + NAD+ | - |
Heliocidaris crassispina | oxaloacetate + NADH + H+ | - |
r | |
malate + NAD+ | - |
Strongylocentrotus purpuratus | oxaloacetate + NADH + H+ | - |
r | |
oxaloacetate + NADH | - |
Heliocidaris crassispina | L-malate + NAD+ | - |
r | |
oxaloacetate + NADH | - |
Strongylocentrotus purpuratus | L-malate + NAD+ | - |
r |
Subunits | Comment | Organism |
---|---|---|
dimer | 2 * 35000, SDS-PAGE | Heliocidaris crassispina |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
45 | - |
after incubation for 50 min enzyme loses about 90% of its activity | Heliocidaris crassispina |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8.2 | 9.4 | reduction of oxaloacetate with NADH | Heliocidaris crassispina |
10.2 | - |
malate oxidation with NAD+ | Heliocidaris crassispina |