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Literature summary for 1.1.1.37 extracted from

  • Okabayashi, K.; Nakano, E.
    Purification and properties of mitochondrial malate dehydrogenase from unfertilized eggs of the sea urchin, Anthocidaris crassispina (1984), J. Biochem., 95, 1625-1632.
    View publication on PubMed

Activating Compound

Activating Compound Comment Organism Structure
dithiothreitol
-
Heliocidaris crassispina
Sodium phosphate
-
Heliocidaris crassispina

Inhibitors

Inhibitors Comment Organism Structure
ADP
-
Heliocidaris crassispina
ATP
-
Heliocidaris crassispina
Na2SO3
-
Heliocidaris crassispina
oxaloacetate substrate inhibition Heliocidaris crassispina
p-chloromercuriphenylsulfonate
-
Heliocidaris crassispina
sulfite
-
Heliocidaris crassispina
Zn2+
-
Heliocidaris crassispina

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.03
-
NADH
-
Heliocidaris crassispina
0.6
-
NAD+
-
Heliocidaris crassispina
7
-
L-malate
-
Heliocidaris crassispina

Localization

Localization Comment Organism GeneOntology No. Textmining
cytosol
-
Heliocidaris crassispina 5829
-
mitochondrion
-
Heliocidaris crassispina 5739
-

Metals/Ions

Metals/Ions Comment Organism Structure
CaCl2
-
Heliocidaris crassispina
MgCl2
-
Heliocidaris crassispina
Na2HAsO4
-
Heliocidaris crassispina
NaF
-
Heliocidaris crassispina

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
35000
-
2 * 35000, SDS-PAGE Heliocidaris crassispina
65000
-
-
Heliocidaris crassispina

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
malate + NAD+ Heliocidaris crassispina
-
oxaloacetate + NADH + H+
-
r
malate + NAD+ Strongylocentrotus purpuratus
-
oxaloacetate + NADH + H+
-
r

Organism

Organism UniProt Comment Textmining
Heliocidaris crassispina
-
sea urchin
-
Strongylocentrotus purpuratus
-
sea urchin
-

Purification (Commentary)

Purification (Comment) Organism
-
Strongylocentrotus purpuratus
M-MDH Heliocidaris crassispina

Source Tissue

Source Tissue Comment Organism Textmining
egg
-
Heliocidaris crassispina
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.28
-
-
Heliocidaris crassispina

Storage Stability

Storage Stability Organism
-20°C, can be stored at least several months without any appreciable loss in activity Heliocidaris crassispina

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
(S)-malate + NAD+
-
Heliocidaris crassispina oxaloacetate + NADH + H+
-
r
(S)-malate + NAD+
-
Strongylocentrotus purpuratus oxaloacetate + NADH + H+
-
r
malate + NAD+
-
Heliocidaris crassispina oxaloacetate + NADH + H+
-
r
malate + NAD+
-
Strongylocentrotus purpuratus oxaloacetate + NADH + H+
-
r
oxaloacetate + NADH
-
Heliocidaris crassispina L-malate + NAD+
-
r
oxaloacetate + NADH
-
Strongylocentrotus purpuratus L-malate + NAD+
-
r

Subunits

Subunits Comment Organism
dimer 2 * 35000, SDS-PAGE Heliocidaris crassispina

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
45
-
after incubation for 50 min enzyme loses about 90% of its activity Heliocidaris crassispina

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8.2 9.4 reduction of oxaloacetate with NADH Heliocidaris crassispina
10.2
-
malate oxidation with NAD+ Heliocidaris crassispina