General Stability | Organism |
---|---|
addition of NAD+, NADH or oxaloacetate stabilizes the enzyme | Rhodobacter capsulatus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.025 | - |
NADH | - |
Rhodobacter capsulatus | |
0.048 | - |
NAD+ | - |
Rhodobacter capsulatus | |
0.085 | - |
oxaloacetate | - |
Rhodobacter capsulatus | |
2.1 | - |
L-malate | - |
Rhodobacter capsulatus | |
2.2 | - |
Ketomalonate | - |
Rhodobacter capsulatus |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
35000 | - |
2 * 35000, SDS-PAGE | Rhodobacter capsulatus |
80000 | - |
gel filtration | Rhodobacter capsulatus |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
malate + NAD+ | Thermus aquaticus | - |
oxaloacetate + NADH + H+ | - |
r | |
malate + NAD+ | Cereibacter sphaeroides | - |
oxaloacetate + NADH + H+ | - |
r | |
malate + NAD+ | Rhodobacter capsulatus | - |
oxaloacetate + NADH + H+ | - |
r |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Cereibacter sphaeroides | - |
- |
- |
Rhodobacter capsulatus | - |
strain 37b, strain number DSM 938 | - |
Thermus aquaticus | - |
- |
- |
Purification (Comment) | Organism |
---|---|
- |
Cereibacter sphaeroides |
- |
Rhodobacter capsulatus |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
348 | - |
- |
Rhodobacter capsulatus |
Storage Stability | Organism |
---|---|
-10°C, in 20 mM potassium phosphate buffer, pH 7.0, 0.01% 2-mercaptoethanol/40% glycerol, can be stored for at least 2 months without loss of activity | Rhodobacter capsulatus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
(S)-malate + NAD+ | - |
Thermus aquaticus | oxaloacetate + NADH + H+ | - |
r | |
(S)-malate + NAD+ | - |
Cereibacter sphaeroides | oxaloacetate + NADH + H+ | - |
r | |
(S)-malate + NAD+ | - |
Rhodobacter capsulatus | oxaloacetate + NADH + H+ | - |
r | |
2-oxobutyrate + NADH | - |
Rhodobacter capsulatus | 2-hydroxybutyrate + NAD+ | - |
? | |
alpha-keto-malonate + NADH | - |
Rhodobacter capsulatus | ? + NAD+ | - |
? | |
malate + NAD+ | - |
Thermus aquaticus | oxaloacetate + NADH + H+ | - |
r | |
malate + NAD+ | - |
Cereibacter sphaeroides | oxaloacetate + NADH + H+ | - |
r | |
malate + NAD+ | - |
Rhodobacter capsulatus | oxaloacetate + NADH + H+ | - |
r | |
meso-tartrate + NAD+ | slightly oxidized, 0.82 of the rate for L-malate oxidation | Rhodobacter capsulatus | ? + NADH | - |
r | |
additional information | D-malate, L-tartarate, D-tartarate, hydroxymalonate, dihydroxyfumarate, L-lactate, D-lactate Dl-alpha-hydroxybutyrate, pyruvate, alpha-hydroxypyruvate, alpha-ketoisovalerate and alpha-ketoglutarate are no substrates | Rhodobacter capsulatus | ? | - |
? | |
oxaloacetate + NADH | - |
Thermus aquaticus | L-malate + NAD+ | - |
r | |
oxaloacetate + NADH | - |
Cereibacter sphaeroides | L-malate + NAD+ | - |
r | |
oxaloacetate + NADH | - |
Rhodobacter capsulatus | L-malate + NAD+ | - |
r |
Subunits | Comment | Organism |
---|---|---|
dimer | - |
Thermus aquaticus |
dimer | 2 * 35000, SDS-PAGE | Rhodobacter capsulatus |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
40 | - |
loses its activity completely upon incubation for 10 min | Rhodobacter capsulatus |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
4.3 | 7 | reduction of ketomalonate | Rhodobacter capsulatus |
7.75 | 8.5 | reduction of oxaloacetate | Rhodobacter capsulatus |
9.5 | - |
L-malate oxidation | Rhodobacter capsulatus |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NAD+ | can not be replaced by NADP+ | Rhodobacter capsulatus |