Cloned (Comment) | Organism |
---|---|
expression in Escherichia coli as protein fusion with glutathione S-transferase or a poly-His tail | Echinococcus granulosus |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
additional information | unaffected by excess of L-malate | Echinococcus granulosus | |
oxaloacetate | above 0.025 mM | Echinococcus granulosus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.004 | - |
oxaloacetate | pH 8.0, native enzyme | Echinococcus granulosus | |
0.007 | - |
NADH | pH 8.0, native enzyme | Echinococcus granulosus | |
0.0127 | - |
NADH | pH 8.0, recombinant enzyme with a poly-His tai | Echinococcus granulosus | |
0.013 | - |
oxaloacetate | pH 8.0, recombinant protein fusions with glutathione S-transferase | Echinococcus granulosus | |
0.018 | - |
oxaloacetate | pH 8.0, recombinant enzyme with a poly-His tail | Echinococcus granulosus | |
0.019 | - |
NADH | pH 8.0, recombinant protein fusions with glutathione S-transferase | Echinococcus granulosus | |
0.027 | - |
NAD+ | pH 7.6, native enzyme | Echinococcus granulosus | |
0.033 | - |
L-malate | pH 7.6, native enzyme | Echinococcus granulosus | |
0.033 | - |
NAD+ | pH 7.6, recombinant enzyme with a poly-His tail | Echinococcus granulosus | |
0.036 | - |
NAD+ | pH 7.6, recombinant protein fusions with glutathione S-transferase | Echinococcus granulosus | |
0.047 | - |
L-malate | pH 7.6, recombinant enzyme with a poly-His tail | Echinococcus granulosus | |
0.054 | - |
L-malate | pH 7.6, native enzyme, recombinant protein fusions with glutathione S-transferase | Echinococcus granulosus |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
mitochondrion | - |
Echinococcus granulosus | 5739 | - |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Echinococcus granulosus | Q704F5 | - |
- |
Purification (Comment) | Organism |
---|---|
- |
Echinococcus granulosus |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
protoscolex | - |
Echinococcus granulosus | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
238.1 | - |
- |
Echinococcus granulosus |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NAD+ | - |
Echinococcus granulosus | |
NADH | - |
Echinococcus granulosus |