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Literature summary for 1.1.1.37 extracted from

  • Park, J.S.; Han, K.Y.; Song, J.A.; Ahn, K.Y.; Seo, H.S.; Lee, J.
    Escherichia coli malate dehydrogenase, a novel solubility enhancer for heterologous proteins synthesized in Escherichia coli (2007), Biotechnol. Lett., 29, 1513-1518.
    View publication on PubMed

Activating Compound

Activating Compound Comment Organism Structure
additional information Escherichia coli proteome response to a heatshock stress causes a 1.6-fold increase of malate dehydrogenase, even under the heat-shock condition, whereas the total number of soluble proteins decreases by about 5% Escherichia coli

Cloned(Commentary)

Cloned (Comment) Organism
gene mdh, expression of the enzyme in strain BL21(DE3) as soluble protein Escherichia coli

Localization

Localization Comment Organism GeneOntology No. Textmining
cytoplasm
-
Escherichia coli 5737
-

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
(S)-malate + NAD+ Escherichia coli
-
oxaloacetate + NADH + H+
-
r

Organism

Organism UniProt Comment Textmining
Escherichia coli P61889 gene mdh
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
(S)-malate + NAD+
-
Escherichia coli oxaloacetate + NADH + H+
-
r

Synonyms

Synonyms Comment Organism
malate dehydrogenase
-
Escherichia coli

Cofactor

Cofactor Comment Organism Structure
NAD+
-
Escherichia coli
NADH
-
Escherichia coli

pI Value

Organism Comment pI Value Maximum pI Value
Escherichia coli 2-dimensional electrophoresis, recombinant enzyme
-
5.4