Literature summary for 1.1.1.37 extracted from

Dong, X.; Fu, M.; Sun, Y.
Refolding of recombinant homodimeric malate dehydrogenase expressed in Escherichia coli as inclusion bodies (2008), Biochem. Eng. J., 38, 341-348.

No PubMed abstract available

Cloned(Commentary)

Cloned (Comment)	Organism
expressed in Escherichia coli as inclusion bodies	Sus scrofa

Localization

Localization	Comment	Organism	GeneOntology No.	Textmining
mitochondrion	-	Sus scrofa	5739	-

Organism

Organism	UniProt	Comment	Textmining
Sus scrofa	-	-	-

Renatured (Commentary)

Renatured (Comment)	Organism
glycerol, sucrose and adenosine triphosphate are effective folding aids, refolding buffer consists of 50 mM Tris-HCl (pH 7.6), 5 mM dithiothreitol, 0.32 mM urea and an additive at a predetermined concentration, such as glycerol (3.2 M), sucrose (1 M), arginine (1 M), glycine (1 M), acetamide (1 M), and ATP (2 mM), refolding at low temperature (4°C) enhances the refolding yield of the homodimeric enzyme. Artificial chaperone-assisted refolding buffer consists of 50 mM Tris-HCl (pH 7.6), 5 mM dithiothreitol, 0.32 M urea, 1.2 (or 0.6) mM cetyltrimethylammonium bromide, and 4.8 mM beta-cyclodextrin.	Sus scrofa

Renatured (Comment)

Organism

glycerol, sucrose and adenosine triphosphate are effective folding aids, refolding buffer consists of 50 mM Tris-HCl (pH 7.6), 5 mM dithiothreitol, 0.32 mM urea and an additive at a predetermined concentration, such as glycerol (3.2 M), sucrose (1 M), arginine (1 M), glycine (1 M), acetamide (1 M), and ATP (2 mM), refolding at low temperature (4°C) enhances the refolding yield of the homodimeric enzyme. Artificial chaperone-assisted refolding buffer consists of 50 mM Tris-HCl (pH 7.6), 5 mM dithiothreitol, 0.32 M urea, 1.2 (or 0.6) mM cetyltrimethylammonium bromide, and 4.8 mM beta-cyclodextrin.

Sus scrofa

Source Tissue

Source Tissue	Comment	Organism	Textmining
heart	-	Sus scrofa	-

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
(S)-malate + NAD+	-	Sus scrofa	oxaloacetate + NADH + H+	-	?

Subunits

Subunits	Comment	Organism
homodimer	x-ray crystallography	Sus scrofa

Synonyms

Synonyms	Comment	Organism
MDH	-	Sus scrofa

Cofactor

Cofactor	Comment	Organism	Structure
NAD+	-	Sus scrofa

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Release 2023.1 (January 2023)