Activating Compound | Comment | Organism | Structure |
---|---|---|---|
additional information | MDH is not affected by DTT or EDTA | Streptomyces coelicolor |
Cloned (Comment) | Organism |
---|---|
gene mdh, sequence comparisons and phylogenetic analysis, overview, overexpression of His6-tagged MDH in Escherichia coli strain Rosetta(DE3), subcloning in Escherichia coli strain DH5alpha | Streptomyces coelicolor |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
(S)-malate | substrate inhibition, 60% inhibition at 30 mM | Streptomyces coelicolor | |
Co2+ | 36% inhibition at 2 mM | Streptomyces coelicolor | |
DMSO | - |
Streptomyces coelicolor | |
Fe2+ | complete inhibition at 2 mM | Streptomyces coelicolor | |
additional information | MDH is not affected by DTT or EDTA | Streptomyces coelicolor | |
oxaloacetate | substrate inhibition, 50% inhibition at 6 mM | Streptomyces coelicolor | |
Triton X-100 | - |
Streptomyces coelicolor | |
Zn2+ | 73% inhibition at 2 mM | Streptomyces coelicolor |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.15 | - |
NAD+ | pH 8.5, 30°C | Streptomyces coelicolor | |
0.189 | - |
oxaloacetate | pH 8.5, 30°C | Streptomyces coelicolor | |
0.494 | - |
(S)-malate | pH 8.5, 30°C | Streptomyces coelicolor | |
0.83 | - |
NADH | pH 8.5, 30°C | Streptomyces coelicolor |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
cytosol | cytosolic MDH | Streptomyces coelicolor | 5829 | - |
mitochondrion | mitochondrial MDH | Streptomyces coelicolor | 5739 | - |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
additional information | enzyme activity is not or poorly affected by 2 mM of Ca2+, Mn2+, Cu2+, Mg2+, Na+, Li+, K+, Rb+, and Ag+ | Streptomyces coelicolor |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
36675 | - |
2 * 36675, MALDI-TOF mass spectrometry | Streptomyces coelicolor |
36675 | - |
4 * 36675, MALDI-TOF mass spectrometry | Streptomyces coelicolor |
73350 | - |
dimeric enzyme, MALDI-TOF mass spectrometry | Streptomyces coelicolor |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
(S)-malate + NAD+ | Streptomyces coelicolor | - |
oxaloacetate + NADH + H+ | - |
r | |
(S)-malate + NAD+ | Streptomyces coelicolor A3(2) | - |
oxaloacetate + NADH + H+ | - |
r |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Streptomyces coelicolor | Q9K3J3 | gene mdh | - |
Streptomyces coelicolor A3(2) | Q9K3J3 | gene mdh | - |
Purification (Comment) | Organism |
---|---|
recombinant His6-tagged MDH from Escherichia coli strain Rosetta(DE3) by cobalt affinity chromatography | Streptomyces coelicolor |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
(S)-malate + NAD+ | - |
Streptomyces coelicolor | oxaloacetate + NADH + H+ | - |
r | |
(S)-malate + NAD+ | the reverse reaction is highly preferred, with a 1050fold higher kcat/Km value | Streptomyces coelicolor | oxaloacetate + NADH + H+ | - |
r | |
(S)-malate + NAD+ | - |
Streptomyces coelicolor A3(2) | oxaloacetate + NADH + H+ | - |
r | |
(S)-malate + NAD+ | the reverse reaction is highly preferred, with a 1050fold higher kcat/Km value | Streptomyces coelicolor A3(2) | oxaloacetate + NADH + H+ | - |
r |
Subunits | Comment | Organism |
---|---|---|
dimer | 2 * 36675, MALDI-TOF mass spectrometry | Streptomyces coelicolor |
More | structure analysis and comparison, MALDI-TOF mass spectrometry, overview | Streptomyces coelicolor |
tetramer | 4 * 36675, MALDI-TOF mass spectrometry | Streptomyces coelicolor |
Synonyms | Comment | Organism |
---|---|---|
(S)-malate dehydrogenase | - |
Streptomyces coelicolor |
More | MDH belongs to the superfamily of 2-ketoacid NAD(P)+-dependent dehydrogenases | Streptomyces coelicolor |
NAD+-dependent MDH | - |
Streptomyces coelicolor |
NAD-MDH | - |
Streptomyces coelicolor |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
50 | - |
reduction and oxidation | Streptomyces coelicolor |
Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|
40 | 60 | profile, overview | Streptomyces coelicolor |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
40 | - |
20 min, purified recombinant enzyme, 82% activity remaining | Streptomyces coelicolor |
50 | - |
purified recombinant enzyme, 70% activity remaining after 20 min, 71% after 30 min, 60% after 90 min, and 50% after 120 min | Streptomyces coelicolor |
55 | - |
20 min, purified recombinant enzyme, 10% activity remaining | Streptomyces coelicolor |
60 | - |
20 min, purified recombinant enzyme, complete inactivation | Streptomyces coelicolor |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
3.66 | - |
NAD+ | pH 8.5, 30°C | Streptomyces coelicolor | |
4.71 | - |
(S)-malate | pH 8.5, 30°C | Streptomyces coelicolor | |
542 | - |
NADH | pH 8.5, 30°C | Streptomyces coelicolor | |
1870 | - |
oxaloacetate | pH 8.5, 30°C | Streptomyces coelicolor |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
6.8 | - |
50°C, phosphate buffer, recombinant enzyme, reduction and oxidation | Streptomyces coelicolor |
8.5 | - |
30°C, Tris-HCl buffer, recombinant enzyme, reduction and oxidation | Streptomyces coelicolor |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
5.7 | 10 | profiles in different buffers, overview | Streptomyces coelicolor |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NAD+ | dependent on and specific for | Streptomyces coelicolor | |
NADH | dependent on and specific for | Streptomyces coelicolor |
Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|
5.8 | - |
oxaloacetate | pH 8.5, 30°C | Streptomyces coelicolor | |
12.8 | - |
(S)-malate | pH 8.5, 30°C | Streptomyces coelicolor |
General Information | Comment | Organism |
---|---|---|
physiological function | mitochondrial and cytosolic MDH isozymes are required for maintaining the balance of NAD+ and NADH in mitochondria and cytosol | Streptomyces coelicolor |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
9.53 | - |
(S)-malate | pH 8.5, 30°C | Streptomyces coelicolor | |
24.4 | - |
NAD+ | pH 8.5, 30°C | Streptomyces coelicolor | |
6560 | - |
NADH | pH 8.5, 30°C | Streptomyces coelicolor | |
10000 | - |
oxaloacetate | pH 8.5, 30°C | Streptomyces coelicolor |