Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 1.1.1.37 extracted from

  • Eprintsev, A.T.; Falaleeva, M.I.; Lyashchenko, M.S.; Toropygin, I.Y.; Igamberdiev, A.U.
    Oligomeric forms of bacterial malate dehydrogenase a study of the enzyme from the phototrophic non-sulfur bacterium Rhodovulum steppense A-20s (2018), Biosci. Biotechnol. Biochem., 82, 81-89 .
    View publication on PubMed
Search for more literature for 1.1.1.37

Inhibitors

Inhibitors Comment Organism Structure
Ba2+
-
 Rhodovulum steppense
Ca2+ inhibits isoform MDH1 at millimolar concentrations; inhibits isoform MDH2 at millimolar concentrations; inhibits isoform MDH3 at millimolar concentrations Rhodovulum steppense
citrate
-
 Rhodovulum steppense
Mn2+ slight inhibition of all isoform MDH2; slight inhibition of isoform MDH1; slight inhibition of isoform MDH3 Rhodovulum steppense
oxaloacetate
-
 Rhodovulum steppense

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.035
-
 oxaloacetate isoform MDH2, at pH 8.5 and 25°C Rhodovulum steppense
0.052
-
 oxaloacetate isoform MDH3, at pH 8.5 and 25°C Rhodovulum steppense
0.06
-
 NAD+ isoform MDH1, at pH 8.5 and 25°C Rhodovulum steppense
0.065
-
 NADH isoform MDH2, at pH 8.5 and 25°C Rhodovulum steppense
0.075
-
 NAD+ isoform MDH3, at pH 8.5 and 25°C Rhodovulum steppense
0.085
-
 NADH isoform MDH3, at pH 8.5 and 25°C Rhodovulum steppense
0.11
-
 NAD+ isoform MDH2, at pH 8.5 and 25°C Rhodovulum steppense
0.2
-
 oxaloacetate isoform MDH1, at pH 8.5 and 25°C Rhodovulum steppense
0.25
-
 NADH isoform MDH1, at pH 8.5 and 25°C Rhodovulum steppense
0.95
-
 (S)-malate isoform MDH1, at pH 8.5 and 25°C Rhodovulum steppense
1.75
-
 (S)-malate isoform MDH3, at pH 8.5 and 25°C Rhodovulum steppense
2.2
-
 (S)-malate isoform MDH2, at pH 8.5 and 25°C Rhodovulum steppense

Metals/Ions

Metals/Ions Comment Organism Structure
Ca2+ Ca2+ slightly (11-15%) activates isoform MDH1 in the micromolar range Rhodovulum steppense
Ca2+ Ca2+ slightly (11-15%) activates isoform MDH2 in the micromolar range Rhodovulum steppense
Ca2+ Ca2+ slightly (11-15%) activates isoform MDH3 in the micromolar range Rhodovulum steppense
Mg2+ activates isoform MDH1 with Ka value of 5.1 mM Rhodovulum steppense
Mg2+ activates isoform MDH2 with Ka value of 8.1 mM Rhodovulum steppense
Mg2+ activates isoform MDH3 with Ka value of 4.7 mM Rhodovulum steppense

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
70000
-
 isoform MDH3, gel filtration Rhodovulum steppense
140000
-
 isoform MDH2, gel filtration Rhodovulum steppense
280000
-
 isoform MDH1, gel filtration Rhodovulum steppense

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
(S)-malate + NAD+ Rhodovulum steppense
-
 oxaloacetate + NADH + H+
-
 r
(S)-malate + NAD+ Rhodovulum steppense A-20s
-
 oxaloacetate + NADH + H+
-
 r
oxaloacetate + NADH + H+ Rhodovulum steppense
-
 (S)-malate + NAD+
-
 r
oxaloacetate + NADH + H+ Rhodovulum steppense A-20s
-
 (S)-malate + NAD+
-
 r

Organism

Organism UniProt Comment Textmining
Rhodovulum steppense
-
-
-
Rhodovulum steppense A-20s
-
-
-

Purification (Commentary)

Purification (Comment) Organism
DEAE cellulose column chromatography and Sephadex G-25 gel filtration Rhodovulum steppense

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
(S)-malate + NAD+
-
 Rhodovulum steppense oxaloacetate + NADH + H+
-
 r
(S)-malate + NAD+
-
 Rhodovulum steppense A-20s oxaloacetate + NADH + H+
-
 r
oxaloacetate + NADH + H+
-
 Rhodovulum steppense (S)-malate + NAD+
-
 r
oxaloacetate + NADH + H+
-
 Rhodovulum steppense A-20s (S)-malate + NAD+
-
 r

Subunits

Subunits Comment Organism
homodimer isoform MDH3, 2 * 35000, SDS-PAGE Rhodovulum steppense
homooctamer isoform MDH1, 8 * 35000, SDS-PAGE Rhodovulum steppense
homotetramer isoform MDH2, 4 * 35000, SDS-PAGE Rhodovulum steppense

Synonyms

Synonyms Comment Organism
Mdh1 isoform Rhodovulum steppense
MDH2 isoform Rhodovulum steppense
Mdh3 isoform Rhodovulum steppense

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
40
-
 temperature optimum of isoform MDH1, with (S)-malate as substrate Rhodovulum steppense
40
-
 temperature optimum of isoform MDH2, with (S)-malate as substrate Rhodovulum steppense
45
-
 temperature optimum of isoform MDH1, with oxaloacetate as substrate Rhodovulum steppense
45
-
 temperature optimum of isoform MDH3, with (S)-malate as substrate Rhodovulum steppense
50
-
 temperature optimum of isoform MDH3, with oxaloacetate as substrate Rhodovulum steppense
55
-
 temperature optimum of isoform MDH2, with oxaloacetate as substrate Rhodovulum steppense

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8
-
 pH optimum of isoform MDH2, with oxaloacetate as substrate Rhodovulum steppense
8.5
-
 pH optimum of isoform MDH3, with oxaloacetate as substrate Rhodovulum steppense
9
-
 pH optimum of isoform MDH2, with (S)-malate as substrate Rhodovulum steppense
9.5
-
 pH optimum of isoform MDH1, with oxaloacetate as substrate Rhodovulum steppense
9.5
-
 pH optimum of isoform MDH3, with (S)-malate as substrate Rhodovulum steppense
10.5
-
 pH optimum of isoform MDH1, with (S)-malate as substrate Rhodovulum steppense

Cofactor

Cofactor Comment Organism Structure
NAD+
-
 Rhodovulum steppense
NADH
-
 Rhodovulum steppense

Ki Value [mM]

Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
0.0019
-
 oxaloacetate isoform MDH2, at pH 8.5 and 25°C Rhodovulum steppense
0.0022
-
 oxaloacetate isoform MDH3, at pH 8.5 and 25°C Rhodovulum steppense
0.008
-
 oxaloacetate isoform MDH1, at pH 8.5 and 25°C Rhodovulum steppense
0.03
-
 citrate isoform MDH2, at pH 8.5 and 25°C Rhodovulum steppense
0.06
-
 citrate isoform MDH1, at pH 8.5 and 25°C Rhodovulum steppense
0.4
-
 citrate isoform MDH3, at pH 8.5 and 25°C Rhodovulum steppense
9
-
 Ba2+ isoform MDH2, at pH 8.5 and 25°C Rhodovulum steppense
11
-
 Ba2+ isoform MDH1, at pH 8.5 and 25°C Rhodovulum steppense
16
-
 Ba2+ isoform MDH3, at pH 8.5 and 25°C Rhodovulum steppense