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Literature summary for 1.1.1.37 extracted from
- Identification and characterization of cytosolic malate dehydrogenase from the liver fluke Fasciola gigantica (2020), Sci. Rep., 10, 13372 .
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expressed in Escherichia coli Codon(+) cells | Fasciola gigantica |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.141 | - |
NADH | at pH 8.0 and 25°C | Fasciola gigantica |  |
| 0.276 | - |
oxaloacetate | at pH 8.0 and 25°C | Fasciola gigantica | |
| 0.309 | - |
NAD+ | at pH 8.0 and 25°C | Fasciola gigantica | |
| 0.447 | - |
(S)-malate | at pH 8.0 and 25°C | Fasciola gigantica | |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| cytosol | - |
Fasciola gigantica | 5829 | - |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| (S)-malate + NAD+ | Fasciola gigantica | - |
oxaloacetate + NADH + H+ | - |
r | |
| oxaloacetate + NADH + H+ | Fasciola gigantica | - |
(S)-malate + NAD+ | - |
r | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Fasciola gigantica | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| Ni-NTA column chromatography | Fasciola gigantica |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| (S)-malate + NAD+ | - |
Fasciola gigantica | oxaloacetate + NADH + H+ | - |
r | |
| oxaloacetate + NADH + H+ | - |
Fasciola gigantica | (S)-malate + NAD+ | - |
r | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| homodimer | 2 * 36000, SDS-PAGE | Fasciola gigantica |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| cytosolic malate dehydrogenase | - |
Fasciola gigantica |
| MDH | - |
Fasciola gigantica |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 40 | - |
above the temperature optimum at 40°C, the activity of the enzyme decreases drastically | Fasciola gigantica |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 10 | - |
in the direction of reaction of malate to oxaloacetate. At a pH of less than 7.0 and more than 10.0, there is an almost complete loss of enzymatic activity | Fasciola gigantica |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| NAD+ | - |
Fasciola gigantica | |
| NADH | - |
Fasciola gigantica | |
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Release 2023.1 (January 2023)
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