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Literature summary for 1.1.1.376 extracted from
- Partial purification and properties of an L-arabinose dehydrogenase from Azospirillium brasilense (1983), Can. J. Microbiol., 29, 242-246.
No PubMed abstract available
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| CoCl2 | 10 mM, 54% inhibition | Azospirillum brasilense |  |
| dithiothreitol | 1 mM, 10% inhibition | Azospirillum brasilense | |
| EDTA | 2.5 mM, 8% inhibition | Azospirillum brasilense | |
| FeSO4 | 10 mM, 30% inhibition | Azospirillum brasilense | |
| MnCl2 | 10 mM, 45% inhibition | Azospirillum brasilense | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.075 | - |
L-arabinose | pH 9,0, 25°C, cofactor: NADP+ | Azospirillum brasilense | |
| 0.14 | - |
L-arabinose | pH 9.0, 25°C, cofactor: NAD+ | Azospirillum brasilense | |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| additional information | no enhancement of activity in presence of divalent cations | Azospirillum brasilense |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 175000 | - |
gel filtration | Azospirillum brasilense |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Azospirillum brasilense | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| partial | Azospirillum brasilense |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 2.6 | - |
pH 9.0, 25°C, L-arabinose + NADP+ | Azospirillum brasilense |
| 4.1 | - |
pH 9.0, 25°C, L-arabinose + NAD+ | Azospirillum brasilense |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| L-arabinose + NAD+ | - |
Azospirillum brasilense | L-arabinono-1,4-lactone + NADH + H+ | - |
? | |
| L-arabinose + NADP+ | - |
Azospirillum brasilense | L-arabinono-1,4-lactone + NADPH + H+ | - |
? | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| L-arabino-aldose dehydrogenase | - |
Azospirillum brasilense |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 25 | - |
assay at | Azospirillum brasilense |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 55 | - |
stable for 5 min | Azospirillum brasilense |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 9.5 | - |
- |
Azospirillum brasilense |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| NAD+ | - |
Azospirillum brasilense | |
| NADP+ | - |
Azospirillum brasilense | |
General Information
| General Information | Comment | Organism |
|---|---|---|
| metabolism | first enzyme in metabolism of L-arabinose | Azospirillum brasilense |
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