Any feedback?
Literature summary for 1.1.1.38 extracted from
- Over-expression, purification, and characterization of recombinant NAD-malic enzyme from Escherichia coli K12 (2007), Protein Expr. Purif., 53, 97-103.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expression of His6-tagged enzyme in strain BL21(DE3) as mainly soluble protein | Escherichia coli K-12 |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.097 | - |
NAD+ | pH 7.2, 25°C, recombinant enzyme | Escherichia coli K-12 |  |
| 0.42 | - |
(S)-malate | pH 7.2, 25°C, recombinant enzyme | Escherichia coli K-12 | |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Mn2+ | required | Escherichia coli K-12 | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 65000 | - |
monomeric recombinant His6-tagged enzyme, native PAGE | Escherichia coli K-12 |
| 260000 | - |
tetrameric recombinant His6-tagged enzyme, native PAGE | Escherichia coli K-12 |
| 500000 | - |
octameric recombinant His6-tagged enzyme, native PAGE | Escherichia coli K-12 |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| (S)-malate + NAD+ | Escherichia coli K-12 | via decarboxylation of oxaloacetate | pyruvate + CO2 + NADH | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Escherichia coli K-12 | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| recombinant His6-tagged enzyme from strain BL21(DE3) by nickel affinity chromatography and ultrafiltration to 95% purity | Escherichia coli K-12 |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| (S)-malate + NAD+ | via decarboxylation of oxaloacetate | Escherichia coli K-12 | pyruvate + CO2 + NADH | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| oligomer | x * 65000, recombinant His6-tagged enzyme, SDS-PAGE, monomer, homotetramer, and homooctamer enzyme fomrs exist in solution | Escherichia coli K-12 |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| NAD+-dependent malic enzyme | - |
Escherichia coli K-12 |
| NAD-malic enzyme | - |
Escherichia coli K-12 |
| NAD-ME | - |
Escherichia coli K-12 |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 25 | - |
assay at | Escherichia coli K-12 |
Turnover Number [1/s]
| Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 134.4 | - |
(S)-malate | pH 7.2, 25°C, recombinant enzyme | Escherichia coli K-12 | |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7.2 | - |
recombinant His6-tagged enzyme | Escherichia coli K-12 |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 6.9 | 8 | over 70% of maximal activity within this range | Escherichia coli K-12 |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| NAD+ | dependent on | Escherichia coli K-12 | |
Use of this online version of BRENDA is free under the CC BY 4.0 license. See terms of use for full details.
Release 2023.1 (January 2023)
Legal
Curated at
Member of
