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Literature summary for 1.1.1.380 extracted from

  • Wichelecki, D.; Vendiola, J.; Jones, A.; Al-Obaidi, N.; Almo, S.; Gerlt, J.
    Investigating the physiological roles of low-efficiency D-mannonate and D-gluconate dehydratases in the enolase superfamily: Pathways for the catabolism of L-gulonate and L-idonate (2014), Biochemistry, 53, 5692-5699.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

Cloned (Comment) Organism
-
Halomonas elongata

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
L-gulonate + NAD+ Halomonas elongata the enzyme participates in a pathway for L-gulonate degradation D-fructuronate + NADH + H+
-
?
L-gulonate + NAD+ Halomonas elongata DSM 2581 the enzyme participates in a pathway for L-gulonate degradation D-fructuronate + NADH + H+
-
?

Organism

Organism UniProt Comment Textmining
Halomonas elongata E1V4Y1
-
-
Halomonas elongata DSM 2581 E1V4Y1
-
-

Purification (Commentary)

Purification (Comment) Organism
-
Halomonas elongata

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
L-gulonate + NAD+
-
Halomonas elongata D-fructuronate + NADH + H+
-
?
L-gulonate + NAD+ the enzyme participates in a pathway for L-gulonate degradation Halomonas elongata D-fructuronate + NADH + H+
-
?
L-gulonate + NAD+
-
Halomonas elongata DSM 2581 D-fructuronate + NADH + H+
-
?
L-gulonate + NAD+ the enzyme participates in a pathway for L-gulonate degradation Halomonas elongata DSM 2581 D-fructuronate + NADH + H+
-
?

Synonyms

Synonyms Comment Organism
HeGulDH
-
Halomonas elongata

Temperature Optimum [┬░C]

Temperature Optimum [┬░C] Temperature Optimum Maximum [┬░C] Comment Organism
25
-
assay at Halomonas elongata

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
1.8
-
L-gulonate pH 7.9, 25┬░C Halomonas elongata

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.9
-
assay at Halomonas elongata

Cofactor

Cofactor Comment Organism Structure
NAD+
-
Halomonas elongata

General Information

General Information Comment Organism
metabolism the enzyme participates in a pathway for L-gulonate degradation Halomonas elongata

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
0.6
-
L-gulonate pH 7.9, 25┬░C Halomonas elongata