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Literature summary for 1.1.1.380 extracted from

  • Kuivanen, J.; Richard, P.
    The yjjN of E. coli codes for an L-galactonate dehydrogenase and can be used for quantification of L-galactonate and L-gulonate (2014), Appl. Biochem. Biotechnol., 173, 1829-1835 .
    View publication on PubMed

Application

Application Comment Organism
analysis YjjN can be applied for a quantitative L-galactonate and L-gulonate detection in a coupled reaction with diaphorase Escherichia coli

Cloned(Commentary)

Cloned (Comment) Organism
gene yjjN, recombinant expression of His-tagged enzyme in Escherichia coli strain BL21(DH3) Escherichia coli

Protein Variants

Protein Variants Comment Organism
additional information deletion of the gene results in a strain with the impaired ability to grow on L-galactonate as a sole carbon source Escherichia coli

Metals/Ions

Metals/Ions Comment Organism Structure
Zn2+ required, zinc enzyme Escherichia coli

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
L-galactonate + NAD+ Escherichia coli preferred substrate D-tagaturonate + NADH + H+
-
?
L-gulonate + NAD+ Escherichia coli
-
D-fructuronate + NADH + H+
-
?

Organism

Organism UniProt Comment Textmining
Escherichia coli Q8XB60
-
-

Purification (Commentary)

Purification (Comment) Organism
recombinant His-tagged enzyme from Escherichia coli strain BL21(DH3) by nickel affinity chromatography Escherichia coli

Source Tissue

Source Tissue Comment Organism Textmining
additional information the strain O157:H7 shows the ability to grow on L-galactonate as a sole carbon source Escherichia coli
-

Storage Stability

Storage Stability Organism
4┬░C, purified recombinant His-tagged enzyme, stability of enzyme YjjN seems to be rather poor since the activity of purified protein decreases dramatically when stored at 4┬░C overnight Escherichia coli

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
L-galactonate + NAD+ preferred substrate Escherichia coli D-tagaturonate + NADH + H+
-
?
L-gulonate + NAD+
-
Escherichia coli D-fructuronate + NADH + H+
-
?
additional information enzyme YjjN is an L-galactonate 5-dehydrogenase having activity also for L-gulonate. Reaction product identification by GC/MS analysis. No activity with substrates of similar structure, L-fuconate and L-lyxonate. And no activity with L-mannonate, L-rhamnonate, D-gulonate, D-galacturonate, D-glucuronate, D-gluconate, D-lyxonate, D-xylonate, D-ribonate, D-arabonate, D-mannonate, D-erythronate, D-glucose, D-xylitol, D-sorbitol, glucooctanic acid, and meso-galactarate. The hydroxyl group at the C6 seems to be essential for the activity Escherichia coli ?
-
?

Synonyms

Synonyms Comment Organism
YjjN
-
Escherichia coli

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8
-
with L-galactonate, recombinant enzyme Escherichia coli

pH Range

pH Minimum pH Maximum Comment Organism
7 9 the optimum pH for the activity with L-galactonate is pH 8.0 while the remaining activity at pH 7.0, pH 7.5, and pH 9.0 is 73%, 97%, and 0%, respectively Escherichia coli

Cofactor

Cofactor Comment Organism Structure
additional information no activity with NADP+ Escherichia coli
NAD+
-
Escherichia coli

Expression

Organism Comment Expression
Escherichia coli transcription of yjjN is upregulated during growth on L-galactonate up

General Information

General Information Comment Organism
evolution YjjN belongs to the zinc-containing alcohol dehydrogenase family. This protein family contains dimeric or tetrameric proteins that coordinate two zinc atoms per subunit. Four conserved cysteine residues, that coordinate the zinc atoms and an aspartate residue predicting NAD+ specificity are found from the YjjN sequence (C92, C95, C98, C106, and D193) Escherichia coli
metabolism the enzyme catalyzes the first step in the hexuronate catabolism in Escherichia coli, overview Escherichia coli