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Literature summary for extracted from

  • Milne, N.; Wahl, S.; van Maris, A.; Pronk, J.; Daran, J.
    Excessive by-product formation: A key contributor to low isobutanol yields of engineered (2016), Metab. Eng. Commun., 3, 39-51.
    View publication on PubMedView publication on EuropePMC


Application Comment Organism
biofuel production a cytosolically located, cofactor-balanced isobutanol pathway, consisting of a mosaic of bacterial enzymes is expressed in Sacchaormyces cerevisiae. In aerobic cultures, the pathway intermediate isobutyraldehyde is oxidized to isobutyrate rather than reduced to isobutanol. Significant concentrations of the pathway intermediates 2,3-dihydroxyisovalerate and alpha-ketoisovalerate, as well as diacetyl and acetoin, accumulate extracellularly. While the engineered strain cannot grow anaerobically, micro-aerobic cultivation results in isobutanol formation at a yield of 0.018 mol/mol glucose. Simultaneously, 2,3-butanediol is produced at a yield of 0.64 mol/molglucose Escherichia coli


Organism UniProt Comment Textmining
Escherichia coli P05793


Synonyms Comment Organism
Escherichia coli