BRENDA - Enzyme Database show
show all sequences of 1.1.1.386

Ipsdienol dehydrogenase (IDOLDH): a novel oxidoreductase important for Ips pini pheromone production

Figueroa-Teran, R.; Welch, W.H.; Blomquist, G.J.; Tittiger, C.; Insect Biochem. Mol. Biol. 42, 81-90 (2012)

Data extracted from this reference:

Cloned(Commentary)
Commentary
Organism
the recombinant enzyme is produced in Sf9 cells using a baculovirus expression system
Ips pini
KM Value [mM]
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.58
-
(4R)-ipsdienol
pH 7.8, 25°C
Ips pini
Localization
Localization
Commentary
Organism
GeneOntology No.
Textmining
cytosol
-
Ips pini
5829
-
Molecular Weight [Da]
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
28000
-
calculated from sequence
Ips pini
Natural Substrates/ Products (Substrates)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
(4R)-ipsdienol + NAD(P)+
Ips pini
the enzyme is involved in pheromon production
ipsdienone + NAD(P)H + H+
-
-
?
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Ips pini
G5DGA8
-
-
Source Tissue
Source Tissue
Commentary
Organism
Textmining
midgut
transcript levels are highest in pheromone producing tissue: the anterior midgut of fed males. The protein is detected only in male midguts
Ips pini
-
Specific Activity [micromol/min/mg]
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
0.0163
-
pH 7.8, 25°C
Ips pini
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
(4R)-ipsdienol + NAD(P)+
the enzyme is involved in pheromon production
729828
Ips pini
ipsdienone + NAD(P)H + H+
-
-
-
?
(4R)-ipsdienol + NAD+
no activity with (4S)-ipsdienol. The enzyme shows similar specific activities with NAD+ and NADP+
729828
Ips pini
ipsdienone + NADH + H+
-
-
-
?
(4R)-ipsdienol + NADP+
no activity with (4S)-ipsdienol. The enzyme shows similar specific activities with NAD+ and NADP+
729828
Ips pini
ipsdienone + NADPH + H+
-
-
-
?
Temperature Optimum [°C]
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
25
-
assay at
Ips pini
pH Optimum
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
7.8
-
assay at
Ips pini
Cofactor
Cofactor
Commentary
Organism
Structure
NAD+
the enzyme shows similar specific activities with NAD+ and NADP+
Ips pini
NADP+
the enzyme shows similar specific activities with NAD+ and NADP+
Ips pini
pI Value
Organism
Commentary
pI Value Maximum
pI Value
Ips pini
calculated from sequence
-
5.8
Cloned(Commentary) (protein specific)
Commentary
Organism
the recombinant enzyme is produced in Sf9 cells using a baculovirus expression system
Ips pini
Cofactor (protein specific)
Cofactor
Commentary
Organism
Structure
NAD+
the enzyme shows similar specific activities with NAD+ and NADP+
Ips pini
NADP+
the enzyme shows similar specific activities with NAD+ and NADP+
Ips pini
KM Value [mM] (protein specific)
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.58
-
(4R)-ipsdienol
pH 7.8, 25°C
Ips pini
Localization (protein specific)
Localization
Commentary
Organism
GeneOntology No.
Textmining
cytosol
-
Ips pini
5829
-
Molecular Weight [Da] (protein specific)
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
28000
-
calculated from sequence
Ips pini
Natural Substrates/ Products (Substrates) (protein specific)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
(4R)-ipsdienol + NAD(P)+
Ips pini
the enzyme is involved in pheromon production
ipsdienone + NAD(P)H + H+
-
-
?
Source Tissue (protein specific)
Source Tissue
Commentary
Organism
Textmining
midgut
transcript levels are highest in pheromone producing tissue: the anterior midgut of fed males. The protein is detected only in male midguts
Ips pini
-
Specific Activity [micromol/min/mg] (protein specific)
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
0.0163
-
pH 7.8, 25°C
Ips pini
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
(4R)-ipsdienol + NAD(P)+
the enzyme is involved in pheromon production
729828
Ips pini
ipsdienone + NAD(P)H + H+
-
-
-
?
(4R)-ipsdienol + NAD+
no activity with (4S)-ipsdienol. The enzyme shows similar specific activities with NAD+ and NADP+
729828
Ips pini
ipsdienone + NADH + H+
-
-
-
?
(4R)-ipsdienol + NADP+
no activity with (4S)-ipsdienol. The enzyme shows similar specific activities with NAD+ and NADP+
729828
Ips pini
ipsdienone + NADPH + H+
-
-
-
?
Temperature Optimum [°C] (protein specific)
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
25
-
assay at
Ips pini
pH Optimum (protein specific)
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
7.8
-
assay at
Ips pini
pI Value (protein specific)
Organism
Commentary
pI Value Maximum
pI Value
Ips pini
calculated from sequence
-
5.8
General Information
General Information
Commentary
Organism
metabolism
the enzyme is involved in pheromon production
Ips pini
General Information (protein specific)
General Information
Commentary
Organism
metabolism
the enzyme is involved in pheromon production
Ips pini
Other publictions for EC 1.1.1.386
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
742807
Figueroa-Teran
High substrate specificity of ...
Ips pini
J. Biochem.
160
141-151
2016
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1
-
-
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6
6
-
-
1
2
-
4
-
-
1
-
-
3
4
-
6
1
1
-
-
-
1
-
-
2
4
-
-
-
-
1
2
-
-
-
-
6
4
6
-
-
1
2
-
-
-
1
-
3
4
-
6
1
1
-
-
-
1
-
-
-
-
3
3
-
-
-
729828
Figueroa-Teran
Ipsdienol dehydrogenase (IDOLD ...
Ips pini
Insect Biochem. Mol. Biol.
42
81-90
2012
-
-
1
-
-
-
-
1
1
-
1
1
-
3
-
-
-
-
-
1
1
-
3
-
1
-
-
-
1
-
-
2
-
1
-
-
-
1
2
-
-
-
-
-
-
1
1
-
1
1
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-
-
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-
1
1
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3
-
1
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1
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1
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1
1
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