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show all sequences of 1.1.1.386

High substrate specificity of ipsdienol dehydrogenase (IDOLDH), a short-chain dehydrogenase from Ips pini bark beetles

Figueroa-Teran, R.; Pak, H.; Blomquist, G.J.; Tittiger, C.; J. Biochem. 160, 141-151 (2016)

Data extracted from this reference:

Cloned(Commentary)
Commentary
Organism
gene IDOLDH, recombinant expression in Spodoptera frugiperda Sf9 cells via baculovirus transfection method
Ips pini
Inhibitors
Inhibitors
Commentary
Organism
Structure
(R)-menthone
-
Ips pini
1,10-phenanthroline
-
Ips pini
1-propanol
-
Ips pini
2-propanol
-
Ips pini
ipsdienone
-
Ips pini
additional information
1,3-butanediol, nerol, geraniol, citral, (S)-ipsdienol do not act as inhibitors
Ips pini
KM Value [mM]
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
additional information
-
additional information
kinetics, recombinant enzyme
Ips pini
0.000008
-
NADP+
pH 7.5, 25°C, recombinant enzyme, with (R)-ipsdienol
Ips pini
0.002
-
ipsdienone
pH 7.5, 25°C, recombinant enzyme
Ips pini
0.003
-
ipsenone
pH 7.5, 25°C, recombinant enzyme
Ips pini
0.009
-
racemic ipsenol
pH 7.5, 25°C, recombinant enzyme
Ips pini
0.527
-
(R)-ipsdienol
pH 7.5, 25°C, recombinant enzyme
Ips pini
Molecular Weight [Da]
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
117000
-
recombinant enzyme, gel filtration
Ips pini
Natural Substrates/ Products (Substrates)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
(R)-ipsdienol + NADP+
Ips pini
stereospecific reaction
ipsdienone + NADPH + H+
-
-
r
racemic ipsenol + NADP+
Ips pini
-
ipsenone + NADPH + H+
-
-
r
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Ips pini
G5DGA8
-
-
Purification (Commentary)
Commentary
Organism
recombinant enzyme about 2.5fold from Sf9 cells by ammonium sulfate fractionation
Ips pini
Source Tissue
Source Tissue
Commentary
Organism
Textmining
additional information
IDOLDH expression pattern, overview
Ips pini
-
Specific Activity [micromol/min/mg]
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
0.05
-
partially purified recombinant enzyme, pH 7.5, 25°C, substrate ipsdienone
Ips pini
0.092
-
partially purified recombinant enzyme, pH 7.5, 25°C, substrate ipsenone
Ips pini
0.196
-
partially purified recombinant enzyme, pH 7.5, 25°C, substrate racemic ipsenol
Ips pini
0.52
0.525
partially purified recombinant enzyme, pH 7.5, 25°C, Tris-HCl or phosphate buffer, substrate (R)-ipsdienol
Ips pini
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
(R)-ipsdienol + NADP+
stereospecific reaction
742807
Ips pini
ipsdienone + NADPH + H+
-
-
-
r
(R)-ipsdienol + NADP+
stereospecific reaction, no activity with (S)-ipsdienol. The oxidation of (R)-ipsdienol is 6.3times higher than that for the reduction of ipsdienone
742807
Ips pini
ipsdienone + NADPH + H+
-
-
-
r
ipsdienone + NADPH + H+
stereospecific reaction
742807
Ips pini
(R)-ipsdienol + NADP+
-
-
-
r
ipsenone + NADPH + H+
-
742807
Ips pini
racemic ipsenol + NADP+
-
-
-
r
additional information
the enzyme stereospecifically reduces both ketones to their corresponding (R)-alcohols. Low activity with (R)-menthone, 3-penten-2-ol, 17beta-estradiol, poor or no activity with (S)-ipsdienol, nerol, geraniol, myrcene, citral, 4-methyl-3-penten-2-one, 4-methyl-2-pentanone, 4-methyl-2-pentanol, acetone, 1,3-butendiol, etanol, 2-propanol, 3-hydroxybutyrate, and 2-oxobutyrate, substrate specificity, overview. Product identification by GC-MS analysis
742807
Ips pini
?
-
-
-
-
racemic ipsenol + NADP+
-
742807
Ips pini
ipsenone + NADPH + H+
-
-
-
r
Subunits
Subunits
Commentary
Organism
homotetramer
4 * 27500, recombinant enzyme, SDS-PAGE, functional enzyme form
Ips pini
Temperature Optimum [°C]
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
25
-
assay at
Ips pini
pH Optimum
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
7.5
-
assay at
Ips pini
Cofactor
Cofactor
Commentary
Organism
Structure
NADP+
-
Ips pini
NADPH
-
Ips pini
Ki Value [mM]
Ki Value [mM]
Ki Value maximum [mM]
Inhibitor
Commentary
Organism
Structure
0.015
-
ipsdienone
pH 7.5, 25°C, recombinant enzyme
Ips pini
0.03
-
(R)-menthone
pH 7.5, 25°C, recombinant enzyme
Ips pini
9.9
-
2-propanol
pH 7.5, 25°C, recombinant enzyme
Ips pini
33.6
-
1-propanol
pH 7.5, 25°C, recombinant enzyme
Ips pini
Cloned(Commentary) (protein specific)
Commentary
Organism
gene IDOLDH, recombinant expression in Spodoptera frugiperda Sf9 cells via baculovirus transfection method
Ips pini
Cofactor (protein specific)
Cofactor
Commentary
Organism
Structure
NADP+
-
Ips pini
NADPH
-
Ips pini
Inhibitors (protein specific)
Inhibitors
Commentary
Organism
Structure
(R)-menthone
-
Ips pini
1,10-phenanthroline
-
Ips pini
1-propanol
-
Ips pini
2-propanol
-
Ips pini
ipsdienone
-
Ips pini
additional information
1,3-butanediol, nerol, geraniol, citral, (S)-ipsdienol do not act as inhibitors
Ips pini
Ki Value [mM] (protein specific)
Ki Value [mM]
Ki Value maximum [mM]
Inhibitor
Commentary
Organism
Structure
0.015
-
ipsdienone
pH 7.5, 25°C, recombinant enzyme
Ips pini
0.03
-
(R)-menthone
pH 7.5, 25°C, recombinant enzyme
Ips pini
9.9
-
2-propanol
pH 7.5, 25°C, recombinant enzyme
Ips pini
33.6
-
1-propanol
pH 7.5, 25°C, recombinant enzyme
Ips pini
KM Value [mM] (protein specific)
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
additional information
-
additional information
kinetics, recombinant enzyme
Ips pini
0.000008
-
NADP+
pH 7.5, 25°C, recombinant enzyme, with (R)-ipsdienol
Ips pini
0.002
-
ipsdienone
pH 7.5, 25°C, recombinant enzyme
Ips pini
0.003
-
ipsenone
pH 7.5, 25°C, recombinant enzyme
Ips pini
0.009
-
racemic ipsenol
pH 7.5, 25°C, recombinant enzyme
Ips pini
0.527
-
(R)-ipsdienol
pH 7.5, 25°C, recombinant enzyme
Ips pini
Molecular Weight [Da] (protein specific)
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
117000
-
recombinant enzyme, gel filtration
Ips pini
Natural Substrates/ Products (Substrates) (protein specific)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
(R)-ipsdienol + NADP+
Ips pini
stereospecific reaction
ipsdienone + NADPH + H+
-
-
r
racemic ipsenol + NADP+
Ips pini
-
ipsenone + NADPH + H+
-
-
r
Purification (Commentary) (protein specific)
Commentary
Organism
recombinant enzyme about 2.5fold from Sf9 cells by ammonium sulfate fractionation
Ips pini
Source Tissue (protein specific)
Source Tissue
Commentary
Organism
Textmining
additional information
IDOLDH expression pattern, overview
Ips pini
-
Specific Activity [micromol/min/mg] (protein specific)
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
0.05
-
partially purified recombinant enzyme, pH 7.5, 25°C, substrate ipsdienone
Ips pini
0.092
-
partially purified recombinant enzyme, pH 7.5, 25°C, substrate ipsenone
Ips pini
0.196
-
partially purified recombinant enzyme, pH 7.5, 25°C, substrate racemic ipsenol
Ips pini
0.52
0.525
partially purified recombinant enzyme, pH 7.5, 25°C, Tris-HCl or phosphate buffer, substrate (R)-ipsdienol
Ips pini
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
(R)-ipsdienol + NADP+
stereospecific reaction
742807
Ips pini
ipsdienone + NADPH + H+
-
-
-
r
(R)-ipsdienol + NADP+
stereospecific reaction, no activity with (S)-ipsdienol. The oxidation of (R)-ipsdienol is 6.3times higher than that for the reduction of ipsdienone
742807
Ips pini
ipsdienone + NADPH + H+
-
-
-
r
ipsdienone + NADPH + H+
stereospecific reaction
742807
Ips pini
(R)-ipsdienol + NADP+
-
-
-
r
ipsenone + NADPH + H+
-
742807
Ips pini
racemic ipsenol + NADP+
-
-
-
r
additional information
the enzyme stereospecifically reduces both ketones to their corresponding (R)-alcohols. Low activity with (R)-menthone, 3-penten-2-ol, 17beta-estradiol, poor or no activity with (S)-ipsdienol, nerol, geraniol, myrcene, citral, 4-methyl-3-penten-2-one, 4-methyl-2-pentanone, 4-methyl-2-pentanol, acetone, 1,3-butendiol, etanol, 2-propanol, 3-hydroxybutyrate, and 2-oxobutyrate, substrate specificity, overview. Product identification by GC-MS analysis
742807
Ips pini
?
-
-
-
-
racemic ipsenol + NADP+
-
742807
Ips pini
ipsenone + NADPH + H+
-
-
-
r
Subunits (protein specific)
Subunits
Commentary
Organism
homotetramer
4 * 27500, recombinant enzyme, SDS-PAGE, functional enzyme form
Ips pini
Temperature Optimum [°C] (protein specific)
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
25
-
assay at
Ips pini
pH Optimum (protein specific)
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
7.5
-
assay at
Ips pini
General Information
General Information
Commentary
Organism
evolution
the enzyme belongs to the short-chain oxidoreductase ipsdienol dehydrogenases
Ips pini
additional information
the enzyme contains the typical Rossmann-fold motif for nucleotide binding and the catalytic tetrad that are highly conserved
Ips pini
physiological function
Ips spp. bark beetles use ipsdienol, ipsenol, ipsdienone and ipsenone as aggregation pheromone components and pheromone precursors. For Ips pini, the short-chain oxidoreductase ipsdienol dehydrogenase (IDOLDH) converts (R)-ipsdienol to ipsdienone, and thus likely plays a role in determining pheromone composition. Different IDOLDH orthologues and or activity levels contribute to differences in Ips spp. pheromone composition
Ips pini
General Information (protein specific)
General Information
Commentary
Organism
evolution
the enzyme belongs to the short-chain oxidoreductase ipsdienol dehydrogenases
Ips pini
additional information
the enzyme contains the typical Rossmann-fold motif for nucleotide binding and the catalytic tetrad that are highly conserved
Ips pini
physiological function
Ips spp. bark beetles use ipsdienol, ipsenol, ipsdienone and ipsenone as aggregation pheromone components and pheromone precursors. For Ips pini, the short-chain oxidoreductase ipsdienol dehydrogenase (IDOLDH) converts (R)-ipsdienol to ipsdienone, and thus likely plays a role in determining pheromone composition. Different IDOLDH orthologues and or activity levels contribute to differences in Ips spp. pheromone composition
Ips pini
Other publictions for EC 1.1.1.386
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
742807
Figueroa-Teran
High substrate specificity of ...
Ips pini
J. Biochem.
160
141-151
2016
-
-
1
-
-
-
6
6
-
-
1
2
-
4
-
-
1
-
-
3
4
-
6
1
1
-
-
-
1
-
-
2
4
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-
-
-
1
2
-
-
-
-
6
4
6
-
-
1
2
-
-
-
1
-
3
4
-
6
1
1
-
-
-
1
-
-
-
-
3
3
-
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-
729828
Figueroa-Teran
Ipsdienol dehydrogenase (IDOLD ...
Ips pini
Insect Biochem. Mol. Biol.
42
81-90
2012
-
-
1
-
-
-
-
1
1
-
1
1
-
3
-
-
-
-
-
1
1
-
3
-
1
-
-
-
1
-
-
2
-
1
-
-
-
1
2
-
-
-
-
-
-
1
1
-
1
1
-
-
-
-
-
1
1
-
3
-
1
-
-
-
1
-
-
1
-
1
1
-
-
-