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Literature summary for 1.1.1.39 extracted from

  • Liu, D.; Hwang, C.C.; Cook, P.F.
    Alternative substrates for malic enzyme: oxidative decarboxylation of L-aspartate (2002), Biochemistry, 41, 12200-12203.
    View publication on PubMed
Search for more literature for 1.1.1.39

Inhibitors

Inhibitors Comment Organism Structure
L-aspartate slightly competitive to malate, only slight inhibition below pH 6.0 Ascaris suum

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
additional information
-
 additional information kinetics Ascaris suum
1
-
 (2S)-malate 25°C Ascaris suum
2
-
 (2R,3R)-erythrofluoromalate 25°C Ascaris suum
20
-
 (2S,3R)-tartrate 25°C, pH 7.8 Ascaris suum
40
-
 meso-tartrate 25°C, pH 7.8 Ascaris suum
60
-
 (2S)-aspartate 25°C Ascaris suum

Metals/Ions

Metals/Ions Comment Organism Structure
Mg2+
-
 Ascaris suum

Organism

Organism UniProt Comment Textmining
Ascaris suum
-
-
-

Reaction

Reaction Comment Organism Reaction ID
(S)-malate + NAD+ = pyruvate + CO2 + NADH + H+ reaction mechanism of oxidative decarboxylation Ascaris suum
a

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
(2R,3R)-erythrofluoromalate + NAD+
-
 Ascaris suum ?
-
 ?
(2S,3R)-tartrate + NAD+
-
 Ascaris suum ?
-
 ?
(S)-malate + NAD+
-
 Ascaris suum pyruvate + CO2 + NADH
-
 ?
L-aspartate + NAD+
-
 Ascaris suum iminopyruvate + CO2 + NADH + H+
-
 ?
meso-tartrate + NAD+
-
 Ascaris suum ?
-
 ?
additional information enzyme shows a strict requirement for 2S-stereochemistry Ascaris suum ?
-
 ?

Synonyms

Synonyms Comment Organism
NAD-malic enzyme
-
 Ascaris suum

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
25
-
 assay at Ascaris suum

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
additional information
-
 pH-dependency of the reaction kinetics, overview Ascaris suum

Cofactor

Cofactor Comment Organism Structure
NAD+
-
 Ascaris suum

Ki Value [mM]

Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
additional information
-
 additional information inhibition kinetics Ascaris suum
58
-
 L-aspartate pH 8.4, 25°C, versus malate Ascaris suum
80
-
 L-aspartate pH 7.0, 25°C, versus malate Ascaris suum