BRENDA - Enzyme Database
show all sequences of 1.1.1.391

Characterization of NAD-dependent 3alpha- and 3beta-hydroxysteroid dehydrogenase and of NADP-dependent 7beta-hydroxysteroid dehydrogenase from Peptostreptococcus productus

Edenharder, R.; Pfutzner, A.; Hammann, R.; Biochim. Biophys. Acta 1004, 230-238 (1989)

Data extracted from this reference:

General Stability
General Stability
Organism
DTT stabilizes, while ETA has no influence on enzyme stability
Blautia producta
Inhibitors
Inhibitors
Commentary
Organism
Structure
Bile acids
specific enzymatic activities are significantly reduced when bacteria are grown in the presence of 3-oxo bile acids, while other bile acids are ineffective
Blautia producta
Localization
Localization
Commentary
Organism
GeneOntology No.
Textmining
membrane
membrane-bound
Blautia producta
16020
-
Molecular Weight [Da]
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
132000
-
-
Blautia producta
Organism
Organism
UniProt
Commentary
Textmining
Blautia producta
-
-
-
Blautia producta K2/25-1
-
-
-
Purification (Commentary)
Purification (Commentary)
Organism
native enzyme by anion exchange chromatography and gel filtration
Blautia producta
Specific Activity [micromol/min/mg]
Specific Activity Minimum [mol/min/mg]
Specific Activity Maximum [mol/min/mg]
Commentary
Organism
0.35
-
pH 9.5, 25C, substrate 3,7-dioxo-5beta-cholan-24-oic acid
Blautia producta
0.38
-
pH 9.5, 25C, substrate 7alpha-hydroxy-3-oxo-5beta-cholan-24-oic acid
Blautia producta
0.59
-
pH 9.5, 25C, substrate 3,12-dioxo-5beta-cholan-24-oic acid
Blautia producta
1
-
pH 9.5, 25C, substrate 7alpha,12alpha-3-oxo-dihydroxy-5beta-cholan-24-oic acid
Blautia producta
2.12
-
pH 9.5, 25C, substrate 3,7,12-trioxo-5beta-cholan-24-oic acid
Blautia producta
2.35
-
pH 9.5, 25C, substrate 3,7-dioxo-5beta-cholan-24-oic acid
Blautia producta
2.45
-
pH 9.5, 25C, substrate 3beta,12alpha-dihydroxy-5beta-cholan-24-oic acid
Blautia producta
2.49
-
pH 9.5, 25C, substrate 12alpha-hydroxy-3-oxo-5beta-cholate
Blautia producta
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
Substrate Product ID
12alpha-hydroxy-3-oxo-5beta-cholan-24-oic acid + NADH + H+
-
286228
Blautia producta
3beta,12alpha-dihydroxy-5beta-cholan-24-oic acid + NAD+
sole pruduct
-
-
?
3,12-dioxo-5beta-cholan-24-oic acid + NADH + H+
-
286228
Blautia producta
3beta-hydroxy-12-oxo-5beta-cholan-24-oic acid + NAD+
-
-
-
?
3,7-dioxo-5beta-cholan-24-oic acid + NADH + H+
-
286228
Blautia producta
3beta-hydroxy-7-oxo-5beta-cholan-24-oic acid + NAD+
-
-
-
?
7alpha,12alpha-dihydroxy-3-oxo-5beta-cholan-24-oic acid + NADH + H+
-
286228
Blautia producta
3beta,7alpha,12alpha-trihydroxy-5beta-cholan-24-oic acid + NAD+
-
-
-
?
7alpha-hydroxy-3-oxo-5beta-cholan-24-oic acid + NADH + H+
-
286228
Blautia producta
3beta,7alpha-dihydroxy-5beta-cholan-24-oic acid + NAD+
-
-
-
?
additional information
no activity with 3alpha,7beta-dihydroxy-5beta-cholan-24-oic acid, 3alpha-hydroxy-7-oxo-5beta-cholan-24-oic acid, 3alpha,7beta-dihydroxytaureate, 7,12-dioxo-5beta-cholanate, 3alpha,12alpha-dihydroxy-7-oxo-5beta-cholanate and 3alpha-hydroxy-7,12-dioxo-5beta-cholanate
286228
Blautia producta
?
-
-
-
-
Synonyms
Synonyms
Commentary
Organism
3beta-HSDH
-
Blautia producta
NAD-dependent 3beta-hydroxysteroid dehydrogenase
-
Blautia producta
Temperature Optimum [C]
Temperature Optimum [C]
Temperature Optimum Maximum [C]
Commentary
Organism
25
-
assay at
Blautia producta
Temperature Stability [C]
Temperature Stability Minimum [C]
Temperature Stability Maximum [C]
Commentary
Organism
4
-
crude cell extract, prepared in 0.1 M Tris-HCl, pH 8.5, 1.0 mM EDTA, and 1.0 mM DTT in absence of bile acid substrate, stable for several hours and detectable for at least 36 h
Blautia producta
37
-
pH 9.5, activity is reduced to about 20% within 1 h
Blautia producta
50
-
pH 9.5, 10 min, loss of 75% activity, the activity is completely lost within about 30 h
Blautia producta
pH Optimum
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
9.5
-
-
Blautia producta
Cofactor
Cofactor
Commentary
Organism
Structure
NAD+
dependent on
Blautia producta
Cofactor (protein specific)
Cofactor
Commentary
Organism
Structure
NAD+
dependent on
Blautia producta
General Stability (protein specific)
General Stability
Organism
DTT stabilizes, while ETA has no influence on enzyme stability
Blautia producta
Inhibitors (protein specific)
Inhibitors
Commentary
Organism
Structure
Bile acids
specific enzymatic activities are significantly reduced when bacteria are grown in the presence of 3-oxo bile acids, while other bile acids are ineffective
Blautia producta
Localization (protein specific)
Localization
Commentary
Organism
GeneOntology No.
Textmining
membrane
membrane-bound
Blautia producta
16020
-
Molecular Weight [Da] (protein specific)
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
132000
-
-
Blautia producta
Purification (Commentary) (protein specific)
Commentary
Organism
native enzyme by anion exchange chromatography and gel filtration
Blautia producta
Specific Activity [micromol/min/mg] (protein specific)
Specific Activity Minimum [mol/min/mg]
Specific Activity Maximum [mol/min/mg]
Commentary
Organism
0.35
-
pH 9.5, 25C, substrate 3,7-dioxo-5beta-cholan-24-oic acid
Blautia producta
0.38
-
pH 9.5, 25C, substrate 7alpha-hydroxy-3-oxo-5beta-cholan-24-oic acid
Blautia producta
0.59
-
pH 9.5, 25C, substrate 3,12-dioxo-5beta-cholan-24-oic acid
Blautia producta
1
-
pH 9.5, 25C, substrate 7alpha,12alpha-3-oxo-dihydroxy-5beta-cholan-24-oic acid
Blautia producta
2.12
-
pH 9.5, 25C, substrate 3,7,12-trioxo-5beta-cholan-24-oic acid
Blautia producta
2.35
-
pH 9.5, 25C, substrate 3,7-dioxo-5beta-cholan-24-oic acid
Blautia producta
2.45
-
pH 9.5, 25C, substrate 3beta,12alpha-dihydroxy-5beta-cholan-24-oic acid
Blautia producta
2.49
-
pH 9.5, 25C, substrate 12alpha-hydroxy-3-oxo-5beta-cholate
Blautia producta
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
ID
12alpha-hydroxy-3-oxo-5beta-cholan-24-oic acid + NADH + H+
-
286228
Blautia producta
3beta,12alpha-dihydroxy-5beta-cholan-24-oic acid + NAD+
sole pruduct
-
-
?
3,12-dioxo-5beta-cholan-24-oic acid + NADH + H+
-
286228
Blautia producta
3beta-hydroxy-12-oxo-5beta-cholan-24-oic acid + NAD+
-
-
-
?
3,7-dioxo-5beta-cholan-24-oic acid + NADH + H+
-
286228
Blautia producta
3beta-hydroxy-7-oxo-5beta-cholan-24-oic acid + NAD+
-
-
-
?
7alpha,12alpha-dihydroxy-3-oxo-5beta-cholan-24-oic acid + NADH + H+
-
286228
Blautia producta
3beta,7alpha,12alpha-trihydroxy-5beta-cholan-24-oic acid + NAD+
-
-
-
?
7alpha-hydroxy-3-oxo-5beta-cholan-24-oic acid + NADH + H+
-
286228
Blautia producta
3beta,7alpha-dihydroxy-5beta-cholan-24-oic acid + NAD+
-
-
-
?
additional information
no activity with 3alpha,7beta-dihydroxy-5beta-cholan-24-oic acid, 3alpha-hydroxy-7-oxo-5beta-cholan-24-oic acid, 3alpha,7beta-dihydroxytaureate, 7,12-dioxo-5beta-cholanate, 3alpha,12alpha-dihydroxy-7-oxo-5beta-cholanate and 3alpha-hydroxy-7,12-dioxo-5beta-cholanate
286228
Blautia producta
?
-
-
-
-
Temperature Optimum [C] (protein specific)
Temperature Optimum [C]
Temperature Optimum Maximum [C]
Commentary
Organism
25
-
assay at
Blautia producta
Temperature Stability [C] (protein specific)
Temperature Stability Minimum [C]
Temperature Stability Maximum [C]
Commentary
Organism
4
-
crude cell extract, prepared in 0.1 M Tris-HCl, pH 8.5, 1.0 mM EDTA, and 1.0 mM DTT in absence of bile acid substrate, stable for several hours and detectable for at least 36 h
Blautia producta
37
-
pH 9.5, activity is reduced to about 20% within 1 h
Blautia producta
50
-
pH 9.5, 10 min, loss of 75% activity, the activity is completely lost within about 30 h
Blautia producta
pH Optimum (protein specific)
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
9.5
-
-
Blautia producta
Other publictions for EC 1.1.1.391
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Synonyms
Temperature Optimum [C]
Temperature Range [C]
Temperature Stability [C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [C] (protein specific)
Temperature Range [C] (protein specific)
Temperature Stability [C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
736859
Devlin
A biosynthetic pathway for a p ...
Eggerthella lenta, Eggerthella lenta ATCC 25559
Nat. Chem. Biol.
11
685-690
2015
-
-
1
-
-
-
-
2
-
-
-
4
-
4
-
-
-
-
-
-
-
-
4
-
2
1
-
-
2
1
-
-
1
-
-
-
-
-
2
2
-
-
-
-
-
-
2
-
-
-
4
-
-
-
-
-
-
-
-
4
-
2
-
-
2
2
-
-
-
-
2
4
-
-
-
736216
Marschall
Human liver class I alcohol de ...
Homo sapiens
Hepatology
31
990-996
2000
-
-
1
-
-
-
-
4
1
-
-
1
-
2
-
-
1
-
-
1
-
-
6
-
4
1
-
-
4
1
-
-
2
-
-
-
-
-
1
2
-
-
-
-
-
-
4
1
-
-
1
-
-
-
1
-
1
-
-
6
-
1
-
-
4
1
-
-
-
-
1
1
-
4
4
286228
Edenharder
Characterization of NAD-depend ...
Blautia producta, Blautia producta K2/25-1
Biochim. Biophys. Acta
1004
230-238
1989
-
-
-
-
-
1
1
-
1
-
1
-
-
2
-
-
1
-
-
-
8
-
6
-
2
1
-
3
-
1
-
-
1
-
-
-
-
-
-
1
-
-
1
-
1
-
-
1
-
1
-
-
-
-
1
-
-
8
-
6
-
1
-
3
-
1
-
-
-
-
-
-
-
-
-
735471
Edenharder
Partial purification and chara ...
Clostridium innocuum, Clostridium innocuum D1144/3
Appl. Environ. Microbiol.
55
1656-1659
1989
-
-
-
-
-
1
1
1
1
-
1
4
-
2
-
-
1
-
-
1
1
1
11
1
2
1
-
1
-
1
-
-
1
-
-
-
-
-
-
1
-
-
1
-
1
-
1
1
-
1
4
-
-
-
1
-
1
1
1
11
1
1
-
1
-
1
-
-
-
1
-
-
1
-
-