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show all sequences of 1.1.1.391

Partial purification and characterization of an NAD-dependent 3beta-hydroxysteroid dehydrogenase from Clostridium innocuum

Edenharder, R.; Pfuetzner, M.; Appl. Environ. Microbiol. 55, 1656-1659 (1989)

Data extracted from this reference:

General Stability
General Stability
Organism
EDTA has no effect enzyme thermal stability
Clostridium innocuum
Inhibitors
Inhibitors
Commentary
Organism
Structure
3-oxo bile acids
the specific enzyme activity is significantly reduced by growth medium supplementation with 3-oxo bile acids, with trisubstituted acids being more effective than disubstituted ones
Clostridium innocuum
KM Value [mM]
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
additional information
-
additional information
Michaelis-Menten kinetis
Clostridium innocuum
Localization
Localization
Commentary
Organism
GeneOntology No.
Textmining
membrane
membrane-bound
Clostridium innocuum
16020
-
Molecular Weight [Da]
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
56000
-
gel filtration
Clostridium innocuum
Natural Substrates/ Products (Substrates)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
3,12-dioxo-5-beta-cholan-24-oic acid + NADH + H+
Clostridium innocuum
-
3beta-hydroxy-12-oxo-5beta-cholan-24-oic acid + NAD+
-
-
?
3,12-dioxo-5-beta-cholan-24-oic acid + NADH + H+
Clostridium innocuum D1144/3
-
3beta-hydroxy-12-oxo-5beta-cholan-24-oic acid + NAD+
-
-
?
7alpha,12alpha-dihydroxy-3-oxo-5beta-cholan-24-oic acid + NADH + H+
Clostridium innocuum
-
3beta,7alpha,12alpha-trihydroxy-5beta-cholan-24-oic acid + NAD+
-
-
?
7alpha,12alpha-dihydroxy-3-oxo-5beta-cholan-24-oic acid + NADH + H+
Clostridium innocuum D1144/3
-
3beta,7alpha,12alpha-trihydroxy-5beta-cholan-24-oic acid + NAD+
-
-
?
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Clostridium innocuum
-
-
-
Clostridium innocuum D1144/3
-
-
-
Purification (Commentary)
Commentary
Organism
native enzyme 6.3fold by anion exchange chromatography and gel filtration
Clostridium innocuum
Source Tissue
Source Tissue
Commentary
Organism
Textmining
additional information
the enzyme production is constitutive in the absence of added bile acids
Clostridium innocuum
-
Specific Activity [micromol/min/mg]
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
3.2
-
purified enzyme, pH 10.0-10.2, 37°C
Clostridium innocuum
Storage Stability
Storage Stability
Organism
4°C, crude enzyme extract, pH 8.5, in the presence of dithiothreitol, no loss of activity after 24 h
Clostridium innocuum
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
12alpha-hydroxy-3-oxo-5beta-cholan-24-oic acid + NADH + H+
-
735471
Clostridium innocuum
3beta,12alpha-dihydroxy-5beta-cholan-24-oic acid + NAD+
sole pruduct
-
-
?
12alpha-hydroxy-3-oxo-5beta-cholan-24-oic acid + NADH + H+
-
735471
Clostridium innocuum D1144/3
3beta,12alpha-dihydroxy-5beta-cholan-24-oic acid + NAD+
sole pruduct
-
-
?
3,12-dioxo-5-beta-cholan-24-oic acid + NADH + H+
-
735471
Clostridium innocuum
3beta-hydroxy-12-oxo-5beta-cholan-24-oic acid + NAD+
-
-
-
?
3,12-dioxo-5-beta-cholan-24-oic acid + NADH + H+
-
735471
Clostridium innocuum D1144/3
3beta-hydroxy-12-oxo-5beta-cholan-24-oic acid + NAD+
-
-
-
?
3,12-dioxo-5beta-cholan-24-oic acid + NADH + H+
-
735471
Clostridium innocuum
3beta-hydroxy-12-oxo-5beta-cholan-24-oic acid + NAD+
-
-
-
?
3,12-dioxo-5beta-cholan-24-oic acid + NADH + H+
-
735471
Clostridium innocuum D1144/3
3beta-hydroxy-12-oxo-5beta-cholan-24-oic acid + NAD+
-
-
-
?
7alpha,12alpha-dihydroxy-3-oxo-5beta-cholan-24-oic acid + NADH + H+
-
735471
Clostridium innocuum
3beta,7alpha,12alpha-trihydroxy-5beta-cholan-24-oic acid + NAD+
-
-
-
?
7alpha,12alpha-dihydroxy-3-oxo-5beta-cholan-24-oic acid + NADH + H+
-
735471
Clostridium innocuum D1144/3
3beta,7alpha,12alpha-trihydroxy-5beta-cholan-24-oic acid + NAD+
-
-
-
?
7alpha-hydroxy-3-oxo-5beta-cholan-24-oic acid + NADH + H+
-
735471
Clostridium innocuum
3beta,7alpha-dihydroxy-5beta-cholan-24-oic acid + NAD+
-
-
-
?
additional information
the enzyme reduces a 3-oxo group in unconjugated and conjugated bile acids. Lower Km values are demonstrated with disubstituted than with trisubstituted bile acids, oxo functions at C-7 and C-12 further reduce the Km value
735471
Clostridium innocuum
?
-
-
-
-
additional information
the enzyme reduces a 3-oxo group in unconjugated and conjugated bile acids. Lower Km values are demonstrated with disubstituted than with trisubstituted bile acids, oxo functions at C-7 and C-12 further reduce the Km value
735471
Clostridium innocuum D1144/3
?
-
-
-
-
Subunits
Subunits
Commentary
Organism
monomer
1 * 56000, SDS-PAGE
Clostridium innocuum
Temperature Optimum [°C]
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
37
-
assay at
Clostridium innocuum
Temperature Stability [°C]
Temperature Stability Minimum [°C]
Temperature Stability Maximum [°C]
Commentary
Organism
50
-
crude enzyme extract, pH 8.5, inactivation after 30 min
Clostridium innocuum
pH Optimum
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
10
10.02
-
Clostridium innocuum
Cofactor
Cofactor
Commentary
Organism
Structure
NAD+
dependent on
Clostridium innocuum
Cofactor (protein specific)
Cofactor
Commentary
Organism
Structure
NAD+
dependent on
Clostridium innocuum
General Stability (protein specific)
General Stability
Organism
EDTA has no effect enzyme thermal stability
Clostridium innocuum
Inhibitors (protein specific)
Inhibitors
Commentary
Organism
Structure
3-oxo bile acids
the specific enzyme activity is significantly reduced by growth medium supplementation with 3-oxo bile acids, with trisubstituted acids being more effective than disubstituted ones
Clostridium innocuum
KM Value [mM] (protein specific)
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
additional information
-
additional information
Michaelis-Menten kinetis
Clostridium innocuum
Localization (protein specific)
Localization
Commentary
Organism
GeneOntology No.
Textmining
membrane
membrane-bound
Clostridium innocuum
16020
-
Molecular Weight [Da] (protein specific)
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
56000
-
gel filtration
Clostridium innocuum
Natural Substrates/ Products (Substrates) (protein specific)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
3,12-dioxo-5-beta-cholan-24-oic acid + NADH + H+
Clostridium innocuum
-
3beta-hydroxy-12-oxo-5beta-cholan-24-oic acid + NAD+
-
-
?
3,12-dioxo-5-beta-cholan-24-oic acid + NADH + H+
Clostridium innocuum D1144/3
-
3beta-hydroxy-12-oxo-5beta-cholan-24-oic acid + NAD+
-
-
?
7alpha,12alpha-dihydroxy-3-oxo-5beta-cholan-24-oic acid + NADH + H+
Clostridium innocuum
-
3beta,7alpha,12alpha-trihydroxy-5beta-cholan-24-oic acid + NAD+
-
-
?
7alpha,12alpha-dihydroxy-3-oxo-5beta-cholan-24-oic acid + NADH + H+
Clostridium innocuum D1144/3
-
3beta,7alpha,12alpha-trihydroxy-5beta-cholan-24-oic acid + NAD+
-
-
?
Purification (Commentary) (protein specific)
Commentary
Organism
native enzyme 6.3fold by anion exchange chromatography and gel filtration
Clostridium innocuum
Source Tissue (protein specific)
Source Tissue
Commentary
Organism
Textmining
additional information
the enzyme production is constitutive in the absence of added bile acids
Clostridium innocuum
-
Specific Activity [micromol/min/mg] (protein specific)
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
3.2
-
purified enzyme, pH 10.0-10.2, 37°C
Clostridium innocuum
Storage Stability (protein specific)
Storage Stability
Organism
4°C, crude enzyme extract, pH 8.5, in the presence of dithiothreitol, no loss of activity after 24 h
Clostridium innocuum
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
12alpha-hydroxy-3-oxo-5beta-cholan-24-oic acid + NADH + H+
-
735471
Clostridium innocuum
3beta,12alpha-dihydroxy-5beta-cholan-24-oic acid + NAD+
sole pruduct
-
-
?
12alpha-hydroxy-3-oxo-5beta-cholan-24-oic acid + NADH + H+
-
735471
Clostridium innocuum D1144/3
3beta,12alpha-dihydroxy-5beta-cholan-24-oic acid + NAD+
sole pruduct
-
-
?
3,12-dioxo-5-beta-cholan-24-oic acid + NADH + H+
-
735471
Clostridium innocuum
3beta-hydroxy-12-oxo-5beta-cholan-24-oic acid + NAD+
-
-
-
?
3,12-dioxo-5-beta-cholan-24-oic acid + NADH + H+
-
735471
Clostridium innocuum D1144/3
3beta-hydroxy-12-oxo-5beta-cholan-24-oic acid + NAD+
-
-
-
?
3,12-dioxo-5beta-cholan-24-oic acid + NADH + H+
-
735471
Clostridium innocuum
3beta-hydroxy-12-oxo-5beta-cholan-24-oic acid + NAD+
-
-
-
?
3,12-dioxo-5beta-cholan-24-oic acid + NADH + H+
-
735471
Clostridium innocuum D1144/3
3beta-hydroxy-12-oxo-5beta-cholan-24-oic acid + NAD+
-
-
-
?
7alpha,12alpha-dihydroxy-3-oxo-5beta-cholan-24-oic acid + NADH + H+
-
735471
Clostridium innocuum
3beta,7alpha,12alpha-trihydroxy-5beta-cholan-24-oic acid + NAD+
-
-
-
?
7alpha,12alpha-dihydroxy-3-oxo-5beta-cholan-24-oic acid + NADH + H+
-
735471
Clostridium innocuum D1144/3
3beta,7alpha,12alpha-trihydroxy-5beta-cholan-24-oic acid + NAD+
-
-
-
?
7alpha-hydroxy-3-oxo-5beta-cholan-24-oic acid + NADH + H+
-
735471
Clostridium innocuum
3beta,7alpha-dihydroxy-5beta-cholan-24-oic acid + NAD+
-
-
-
?
additional information
the enzyme reduces a 3-oxo group in unconjugated and conjugated bile acids. Lower Km values are demonstrated with disubstituted than with trisubstituted bile acids, oxo functions at C-7 and C-12 further reduce the Km value
735471
Clostridium innocuum
?
-
-
-
-
additional information
the enzyme reduces a 3-oxo group in unconjugated and conjugated bile acids. Lower Km values are demonstrated with disubstituted than with trisubstituted bile acids, oxo functions at C-7 and C-12 further reduce the Km value
735471
Clostridium innocuum D1144/3
?
-
-
-
-
Subunits (protein specific)
Subunits
Commentary
Organism
monomer
1 * 56000, SDS-PAGE
Clostridium innocuum
Temperature Optimum [°C] (protein specific)
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
37
-
assay at
Clostridium innocuum
Temperature Stability [°C] (protein specific)
Temperature Stability Minimum [°C]
Temperature Stability Maximum [°C]
Commentary
Organism
50
-
crude enzyme extract, pH 8.5, inactivation after 30 min
Clostridium innocuum
pH Optimum (protein specific)
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
10
10.02
-
Clostridium innocuum
Expression
Organism
Commentary
Expression
Clostridium innocuum
effect of bile acids on the production of 3beta-HSDH activity in Clostridium innocuum strain D1144/3
down
Expression (protein specific)
Organism
Commentary
Expression
Clostridium innocuum
effect of bile acids on the production of 3beta-HSDH activity in Clostridium innocuum strain D1144/3
down
Other publictions for EC 1.1.1.391
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
736859
Devlin
A biosynthetic pathway for a p ...
Eggerthella lenta, Eggerthella lenta ATCC 25559
Nat. Chem. Biol.
11
685-690
2015
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-
1
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2
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4
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4
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-
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4
-
1
-
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2
1
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1
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-
2
2
-
-
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2
-
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4
-
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-
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-
-
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4
-
2
-
-
2
2
-
-
-
-
2
4
-
-
-
736216
Marschall
Human liver class I alcohol de ...
Homo sapiens
Hepatology
31
990-996
2000
-
-
1
-
-
-
-
4
1
-
-
1
-
2
-
-
1
-
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1
-
-
6
-
1
-
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4
1
-
-
2
-
-
-
-
-
1
2
-
-
-
-
-
-
4
1
-
-
1
-
-
-
1
-
1
-
-
6
-
1
-
-
4
1
-
-
-
-
1
1
-
4
4
286228
Edenharder
Characterization of NAD-depend ...
Blautia producta, Blautia producta K2/25-1
Biochim. Biophys. Acta
1004
230-238
1989
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-
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1
1
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1
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1
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2
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1
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8
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6
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1
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3
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1
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1
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1
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1
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1
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1
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1
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1
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8
-
6
-
1
-
3
-
1
-
-
-
-
-
-
-
-
-
735471
Edenharder
Partial purification and chara ...
Clostridium innocuum, Clostridium innocuum D1144/3
Appl. Environ. Microbiol.
55
1656-1659
1989
-
-
-
-
-
1
1
1
1
-
1
4
-
2
-
-
1
-
-
1
1
1
11
1
1
-
1
-
1
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1
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-
1
-
-
1
-
1
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1
1
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1
4
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1
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1
1
1
11
1
1
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1
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1
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1
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-
1
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